Results 91 to 100 of about 5,227 (180)

Flavivirus NS1 protein: Selection of a capture DNA aptamer by CE SELEX

Evidência
The clinically important viruses in the genus Flavivirus include Zika virus (ZIKV), dengue virus (DENV), and yellow fever virus (YFV). Accurately diagnosing flaviviral infection is challenging, especially in regions where multiple flaviviruses co ...
Alessandra Nunes Duarte Miranda, Mariane Izabella Abreu de Melo, Camila Maria de Souza Lacerda, Antero Silva Ribeiro de Andrade   +3 more
doaj  

The Effect of RNA Nucleic Acid Aptamers on the Aggregation of Green Fluorescent Protein [PDF]

, 2017
Protein aggregation can impact the product quality of pharmaceutical drugs in terms of efficacy, safety, and immunogenicity leading to economic and technical problems for biotechnology and pharmaceutical companies.
Nguyen, Thomas
core   +1 more source

Research Progress in Small-Molecule Detection Using Aptamer-Based SERS Techniques

Biosensors
Nucleic acid aptamers are single-stranded oligonucleotides that are selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries.
Li Zheng, Qingdan Ye, Mengmeng Wang, Fan Sun, Qiang Chen, Xiaoping Yu, Yufeng Wang, Pei Liang   +7 more
doaj   +1 more source

Insights into the G-rich VEGF-binding aptamer V7t1: when two G-quadruplexes are better than one! [PDF]

, 2019
The G-quadruplex-forming VEGF-binding aptamer V7t1 was previously found to be highly polymorphic in a K+-containing solution and, to restrict its conformational preferences to a unique, well-defined form, modified nucleotides (LNA and/or UNA) were ...
Leone, S., Moccia, F., Montesarchio, D., Musumeci, D., Oliva, R., Petraccone, L., Riccardi, C.   +6 more
core   +1 more source

Characterization of an Aptamer Targeting Neu5Gc, as an Endogenous Pathogenic Factor Derived from Red Meat

Molecules
N-glycolylneuraminic acid (Neu5Gc), a sialic acid predominantly found in the non-neurohumoral fluids of hind-mouthed animals, is incapable of synthesizing Neu5Gc due to a deletion in the CMAH exon of the gene encoding human CMP-Neu5Gc hydroxylase.
Yuxi Guo, Honglin Ren, Han Wang, Yiran Xiao, Cong Wang, Mengdi Liu, Fuchun Duan, Haosong Li, Pan Hu, Yansong Li, Zengshan Liu, Shiying Lu   +11 more
doaj   +1 more source

A novel hybrid approach to overcome defects of CE-SELEX and cell-SELEX in developing aptamers against aspartate β-hydroxylase

Research in Pharmaceutical Sciences
Background and purpose: Aptamers, a new category of molecular probes, are overthrowing antibodies in molecular diagnostics. However, there are serious problems with using aptamers for this application including poor or non-specific binding in vivo ...
Hadi Bakhtiari, Hamed Naghoosi, Sina Sattari, Mahmoud Vahidi, Mehdi Shakouri Khomartash, Ali Faridfar, Mohsen Rajaeinejad, Mohsen Nikandish   +7 more
doaj   +1 more source

Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production [PDF]

, 2012
Aptamers are ssDNA or RNA that binds to wide variety of target molecules with high affinity and specificity producedby systematic evolution of ligands by exponential enrichment (SELEX).
Chee-Hock Hoe1, Junji Tominaga4, Marimuthu Citartan1, Rajan Saini, Soo-Choon Tan, Subash C.B. Gopinath4, Thean-Hock Tang1   +6 more
core  

Aptamer-Based Sensor for Rapid and Sensitive Detection of Ofloxacin in Meat Products

Sensors
Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price and broad spectrum of bacterial inhibition, etc. However, it is difficult to degrade and is retained in animal-derived food products, which are hazardous to human ...
Siyuan Wang, Xiuli Bao, Mingwang Liu, Qingfang Hao, Shuai Zhang, Siying Li, Lei Zhang, Xinxin Kang, Mingsheng Lyu, Shujun Wang   +9 more
doaj   +1 more source

Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program. [PDF]

, 2019
Brown adipose tissue harbors UCP1 to dissipate chemical energy as heat. However, the transcriptional network that governs the thermogenic gene program is incompletely understood. Zc3h10, a CCCH-type zinc finger protein, has recently been reported to bind
Dempersmier, Jon, Dinh, Jennie, Nguyen, Hai, SUL, Hei Sook, Tabuchi, Chihiro, Viscarra, Jose, Wang, Yuhui, Yi, Danielle   +7 more
core  

Etudes des assemblages biomoléculaires par RMN [PDF]

, 2008
L’étude des assemblages biomoléculaires de grande taille a été initiée au cours de la thèse de R. Sounier (bourse ministère de la recherche) dont j’ai assuré la formation et la co-direction depuis octobre 2004 avec Dr. Jean-Pierre Simorre (IBS, Grenoble).
Boisbouvier, Jérôme
core   +2 more sources