Results 11 to 20 of about 5,227 (180)

Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and Biosensing. [PDF]

Adv Sci (Weinh)
The advent of aptamers has highlighted their potential as alternatives to antibodies, overcoming limitations of structural instability and production cost. However, conventional approaches such as SELEX remain slow and labor‐intensive. This review examines recent advances in aptamer engineering, emphasizing in vitro and AI‐driven in silico strategies ...
Nguyen JVL, Meziadi A, Brunner C, McKeague M, Malic L, Tabrizian M.   +5 more
europepmc   +2 more sources

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

Biosensors, 2022
Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment.
Sin Yu Lam, Hill Lam Lau, Chun Kit Kwok
doaj   +1 more source

In Silico discovery of aptamers with an enhanced library design strategy

Computational and Structural Biotechnology Journal, 2023
With advances in force fields and algorithms, robust tools have been developed for molecular simulation of three-dimensional structures of nucleic acids and investigation of aptamer-target interactions.
Long Chen, Bibi Zhang, Zengrui Wu, Guixia Liu, Weihua Li, Yun Tang   +5 more
doaj   +1 more source

AptaTrans: a deep neural network for predicting aptamer-protein interaction using pretrained encoders

BMC Bioinformatics, 2023
Background Aptamers, which are biomaterials comprised of single-stranded DNA/RNA that form tertiary structures, have significant potential as next-generation materials, particularly for drug discovery.
Incheol Shin, Keumseok Kang, Juseong Kim, Sanghun Sel, Jeonghoon Choi, Jae-Wook Lee, Ho Young Kang, Giltae Song   +7 more
doaj   +1 more source

Selection and Characterization of SARS-CoV-2 Spike Binding Clickmers. [PDF]

Chembiochem
Split‐combine click‐systematic evolution of ligands by exponential enrichment against wild‐type SARS‐CoV‐2 spike glycoprotein yields benzofuran‐ and indole‐functionalized DNA aptamers (clickmers). These clickmers retain specific binding to multiple variants with nanomolar affinity, and the 31 nt indole‐functionalized truncate T2 retains binding to ...
Moradzadeh N, Jonczyk A, Schmitz A, Fieberg V, Civit L, Valero J, Famulok M, Mayer G.   +7 more
europepmc   +2 more sources

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Baugh, Bauke W. Dijkstra, Bing, Bock, Cao, Chayen, Convery, Doudna, Ellington, Friedmann, Garber, Hauke Smidt, Hermann, Hianik, Hoggan, Hollis, Horn, Huang, Huang, Hwang, Jiang, Johan Hekelaar, John van der Oost, Kaur, Ke, Kelly, Kikin, Krauss, Kwan, Laing, Lebruska, Lee, Long, Lupold, Macaya, Mark Levisson, Mascini, McPherson, Mehta, Miyakawa, Moorthy, Murai, Nix, Nomura, Orlova, Padmanabhan, Padmanabhan, Paige, Parisien, Poniková, Reinemann, Reinstein, Renault, Rivas, Rowsell, Ruigrok, Sekiya, Shum, Skrzypczak-Jankun, Snyder, Someya, Stoltenburg, Sugiyama, Sussman, Tereshko, Tuerk, Vincent J. B. Ruigrok, Wang, Wilson, Win, Wochner, Yan, Yee, Zuker   +73 more
core   +10 more sources

Screening of Single-Stranded DNA Aptamer Specific for Florfenicol and Application in Detection of Food Safety

Biosensors, 2022
In this work, the single-stranded DNA (ssDNA) aptamers specific to florfenicol (FF) and having a high binding affinity were prepared using the magnetic bead-based systematic evolution of ligands by the exponential enrichment technique (MB-SELEX).
Minghui Shi, Ruobing Liu, Fuyuan Zhang, Bimal Chitrakar, Xianghong Wang   +4 more
doaj   +1 more source

CS-SELEX generates high-affinity ssDNA aptamers as molecular probes for hepatitis C virus envelope glycoprotein E2. [PDF]

PLoS ONE, 2009
Currently, the development of effective diagnostic reagents as well as treatments against Hepatitis C virus (HCV) remains a high priority. In this study, we have described the development of an alive cell surface--Systematic Evolution of Ligands by ...
Fang Chen, Yilan Hu, Dongqing Li, Haidan Chen, Xiao-Lian Zhang   +4 more
doaj   +1 more source

Selection and Characterization of DNA Aptamers for Constructing Aptamer-AuNPs Colorimetric Method for Detection of AFM1

Foods, 2022
Aflatoxin M1 (AFM1), one of the most toxic mycotoxins, is a feed and food contaminant of global concern. To isolate the ssDNA aptamer of AFM1, synthesized magnetic graphene oxide nanomaterials, 12 rounds of subtractive systematic evolution of ligands by ...
Ruobing Liu, Fuyuan Zhang, Yaxin Sang, Minxuan Liu, Minghui Shi, Xianghong Wang   +5 more
doaj   +1 more source

RNA aptamers generated against oligomeric Abeta40 recognize common amyloid aptatopes with low specificity but high sensitivity. [PDF]

, 2009
Aptamers are useful molecular recognition tools in research, diagnostics, and therapy. Despite promising results in other fields, aptamer use has remained scarce in amyloid research, including Alzheimer's disease (AD).
Bitan, Gal, Chen, Chi-Hong B, Murakami, Kazuma, Rahimi, Farid, Summers, Jamie L   +4 more
core   +5 more sources