Results 91 to 100 of about 65,174 (128)

The Sephadex story

Polymer Engineering & Science, 1998
AbstractThe environment and the circumstances leading to the Sephadex group of products for gel filtration (SEC, size exclusion chromatography) are described. The importance of Porath's and the author's experience from zone electrophoresis is stressed.
openaire   +1 more source

Chromatography of cephalosporins on DEAE-Sephadex

Journal of Chromatography A, 1976
A method is described for separating and quantifying new cephalosporin derivatives by means of column chromatography on DEAE-Sephadex A-25. The procedure has been used to determine some breakdown products of cefacetril.
G, Tortolani, E, Romagnoli
openaire   +2 more sources

Separation of lipids on methylated sephadex

Analytical Biochemistry, 1965
Abstract Methylated Sephadex G-25 and G-50 have been prepared. These materials are useful for the separation of lipid-soluble compounds. The separation of phospholipids, triglycerides, cholesterol esters, cholesterol, fatty acids, bile acids, and some polymers has been studied. The separations appear to be based partly on differences in size and/or
E, NYSTROEM, J, SJOEVALL
openaire   +2 more sources

Isoelectric focusing in a Sephadex column

Analytical Biochemistry, 1976
Abstract An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5–10 reaches equilibrium in approximately 5 hr and thereby reduces the ...
T J, O'Brien, H H, Liebke, H S, Cheung, L K, Johnson   +3 more
openaire   +2 more sources

Fractionation of starch dextrins on sephadex

Archives of Biochemistry and Biophysics, 1962
Abstract Partial resolution of a number of starch dextrins on Sephadex columns was achieved. The dextrins examined were Naegeli amylodextrin, an α-amylase digest of amylose and soluble starch. Resolution according to molecular size was demonstrated in these systems by iodine-staining power and by paper chromatography. Amylodextrin could be completely
openaire   +2 more sources

A sephadex extraction of urinary steroid conjugates

Steroids, 1966
Abstract A simple method for the isolation of the C 19 and C 21 steroid conjugates from urine with Sephadex G-50 (coarse) is described. Following contraction of the urine volume by Sephadex, the Sephadex cake was washed with ethanol: methanoi (1:1 v/v) mixture and this was added to the contracted urine sample and evaporated to dryness.
D, Gupta, J, Goodwin
openaire   +2 more sources

Affinity of Mutarotase for Sephadex and Glycogen

Enzyme, 1984
It was found that hog kidney mutarotase has an affinity for Sephadex G-100, equilibrated and eluted with 5 mmol/l EDTA buffer (pH 7.4), resulting in retardation of its elution from the column. The affinity was reduced depending upon the presence of 0.15 mol/l NaCl and 0.2 mol/l glucose, separately or in combination.
I, Miwa, Y, Toyoda, J, Okuda
openaire   +2 more sources

Benzoylated DEAE-Sephadex. Its preparation and application

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
Abstract : In the goal of synthesizing a DNA molecule for biological studies, large amounts of protected di- and trinucleotides were required. The large scale syntheses (2-10 mmoles) of these starting blocks required chromatography through large columns of benzoylated DEAE-cellulose. This is a time-consuming and laborious step.
Michniewicz, J., Bhanot, O., Goodchild, J., Dheer, S., Wightman, R., Narang, S.   +5 more
openaire   +3 more sources

Adsorption chromatography of phenylalanine peptides on Sephadex

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
The adsorption properties of dextran gels have been utilized to separate the first six members of an homologous series of phenylalanine peptides, the longer members being more highly retarded. Separation is influenced by gel structure, pH and the ionic environment.
R K, Bretthauer, A M, Golichowski
openaire   +2 more sources

Partition Chromatography of Oxytocin on ‘Sephadex’

Nature, 1964
GEL-FILTRATION on ‘Sephadex’ as a means of purifying natural products has been widely used since its first introduction by Porath and Flodin1. One of its many applications has been to the purification of natural oxytocin2. Experiments conducted during the past 2 years have shown that the purification of oxytocin can be conveniently and effectively ...
openaire   +2 more sources