Osmometry with single Sephadex beads [PDF]
1. The measurement of osmotic pressure by means of a single bead of Sephadex (Edmond, Farquhar, Dunstone & Ogston, 1968) has been made more precise by immobilizing the bead on a fine needle. The design, calibration and use of the osmometer are described. 2.
A G, Ogston, J D, Wells
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Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core +1 more source
Purification of rat pancreatic lipase
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj +1 more source
A clear separation of retinol, retinal, and retinoic acid has been achieved by liquid–gel partition chromatography on Sephadex LH-20 with solvent mixtures of chloroform, Skellysolve B, and methanol.
Y.L. Ito +3 more
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Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review [PDF]
The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls.
Shailendra Chaurasia +4 more
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One-step chromatographic purification of Helicobacter pylori neutrophil-activating protein expressed in Bacillus subtilis. [PDF]
Helicobacter pylori neutrophil-activating protein (HP-NAP), a major virulence factor of Helicobacter pylori (H. pylori), is capable of activating human neutrophils to produce reactive oxygen species (ROS) and secrete inammatory mediators.
Kuo-Shun Shih +6 more
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Purification and properties of acid phosphatase from Avena elatior L. seeds
Acid phosphatase F1 from Avena elatior seeds was isolated and partially purified by means of alcohol precepitation, DEAE-, CM-column chromatography, Sephadex G-150, Sephadex G-200 and Sepharose 4B - gel filtration.
E. Wieczorek +2 more
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Gel permeation chromatography of neutral hydroxy lipids on Sephadex LH-20
Gel-permeation chromatography on Sephadex LH-20, using ethanol as eluent, permits the resolution of neutral hydroxy lipids according to molecular size.
M. Calderon, W.J. Baumann
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Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques [PDF]
The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml.
Nawal Thnoon Youis +2 more
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Extraction, Structural Characterization and Biological Activities of Polysaccharides Derived From <i>Hericium erinaceus</i>. [PDF]
This review summarizes the methods used to extract and purify H. erinaceus polysaccharides (HEP), primarily hot water extraction and column chromatography. Structural characterization indicates that the molecular weights of HEP range from 2.1 to 75,000 kDa, with glucose as the dominant monosaccharide and β‐glycosidic bonds, notably β‐glucans with 1 → 3
Sun N, Li C, Liang W, Wu Z, Han X, Xu S.
europepmc +2 more sources

