Results 51 to 60 of about 105,930 (282)

Co‐cultivation and Medium Optimization: A Strategy for Discovering Fungal‐derived Protease Inhibitors

Chemistry &Biodiversity, EarlyView.
ABSTRACT The fungi Fusarium guttiforme and Phytophthora palmivora were cultivated in four different media (Potato Dextrose Agar, Czapek, rice, and ISP2) and co‐cultured to stimulate fungal interactions and enhance secondary metabolite production. Promising extracts were fractionated, yielding compounds such as the iron complex of fusaric acid (1 ...
Vitor de Souza Mazucato, Ludmilla Tonani, Marcia Regina von Zeska Kress, Adriano Defini Andricopulo, Gisele Barbosa, Renata Krogh, Leonardo Luiz Gomes Ferreira, Paulo Cezar Vieira   +7 more
wiley   +1 more source

Salix daphnoides: A Screening for Oligomeric and Polymeric Proanthocyanidins

Molecules, 2015
In the present study, a qualitative analysis of proanthocyanidins (PAs) from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1), B2 (2), B3 (3), B4 (4), C1 (5), epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (6)
Stefan Wiesneth, Frank Petereit, Guido Jürgenliemk   +2 more
doaj   +1 more source

Tandem Mass Spectrometry and Bio‐Guided Isolation of Secondary Metabolites With Antiplasmodial Activity From Dalbergia miscolobium Bark

Chemistry &Biodiversity, EarlyView.
ABSTRACT Strategies have been employed to address antimalarial drug resistance, including the exploration of new therapeutic targets. In this study, the stem bark of Dalbergia miscolobium was investigated using in vitro assays against Plasmodium falciparum and pyruvate kinase II (PyrKII), an essential enzyme for parasite development.
Thais Bertolino Vieira Dantas, Igor M. R. Moura, Romário Pereira da Costa, Guilherme Eduardo de Souza, Richele Priscila Severino, Hélder Nagai Consolaro, Luis Felipe de Oliveira, Vinicius Bonatto, Quezia Bezerra Cass, Rafael V. C. Guido, Lorena Ramos Freitas de Sousa   +10 more
wiley   +1 more source

High performance thin-layer chromatography and densitometry of synaptic plasma membrane lipids.

Journal of Lipid Research, 1989
Previously, it has been shown that phospholipids, cholesterol, and glycolipids could be quantitated using the same high performance thin-layer chromatography (HPTLC) method.
W G Wood, M Cornwell, L S Williamson
doaj   +1 more source

Exploring the Antiproliferative Activity of Flavolignans From the Leaves of Casearia arborea (Salicaceae)

Chemistry &Biodiversity, EarlyView.
ABSTRACT The Casearia genus (Salicaceae) is well known as a source of natural antitumor prototypes, as described for clerodane diterpenes. In this way, the dichloromethane phase from leaves of Casearia arborea was phytochemically evaluated in liquid chromatography coupled to high‐resolution mass spectrometry, furnishing flavonoid, flavolignan, and ...
Augusto L. Santos, Mariana T. Rodrigues, Ana Paula Michelli, Rodrigo E. Tamura, Ileana G. S. de Rubió, Marisi G. Soares, Marcelo J. P. Ferreira, Patricia Sartorelli   +7 more
wiley   +1 more source

Analysis of metabolic profiles of bile acids in urine using a lipophilic anion exchanger and computerized gas-liquid chromatorgaphy-mass spectrometry

Journal of Lipid Research, 1977
A method is described for quantitative analysis of bile acids in urine. Urine is acidified and bile acids are extracted on an Amberlite XAD-2 column.
B Almé, A Bremmelgaard, J Sjövall, P Thomassen   +3 more
doaj   +1 more source

Bioprospecting of Labdane‐Type Diterpenes From Austroeupatorium laetevirens: Molecular Networking, Structural Elucidation, and Biological Activity Evaluation

Chemistry &Biodiversity, EarlyView.
ABSTRACT Labdane‐type diterpenes are the main specialized metabolites in the Austroeupatorium genus, known for diverse biological activities. This study employed molecular networking to facilitate the identification of labdanes in Austroeupatorium laetevirens, alongside isolation, structural elucidation, and evaluation of anti‐Mycobacterium ...
Francielli A. P. Valeze, Maria Eduarda V. de Souza, Beatriz P. Moreno, Bianca D. B. Sahm, Andressa L. Ieque, Cleverton S. Fernandes, Regiane B. L. Scodro, Leticia V. Costa‐Lotufo, Marta R. B. do Carmo, Andrea N. L. Batista, João M. Batista Junior, Ernani A. Basso, Maria H. Sarragiotto, Debora C. Baldoqui   +13 more
wiley   +1 more source

Protein kinase-mediated phosphorylation of a purified sterol ester hydrolase from bovine adrenal cortex.

Journal of Lipid Research, 1978
Sterol ester hydrolase (cholesterol esterase, E.C. 3.1.1.13) of bovine adrenal cortex has been extensively purified by ammonium sulfate fractionation, acid precipitation, hydroxylapatite chromatography, and Sephadex G-200 chromatography.
S Naghshineh, C R Treadwell, L L Gallo, G V Vahouny   +3 more
doaj   +1 more source

Analysis of the Effects of Sugar Modifications on RNA Chemical Ligation Reactions

ChemBioChem, EarlyView.
The effect of substitutions at the 2′ position of the nucleotides at the ligation site on phosphoramidate‐type chemical ligation reaction efficiency is examined. The efficiency varies depending on the substituents, with the combination of a fluorine group on 5′NRNA and an O‐methyl group on RNA3′P showing the highest efficiency. Chemical ligation of RNA
Yu Hirano, Naoshi Kojima, Harei Sakurai, Emi Saito, Hirokazu Nankai, Yasuo Komatsu   +5 more
wiley   +1 more source

Antifungal Activity of an Abundant Thaumatin-Like Protein from Banana against Penicillium expansum, and Its Possible Mechanisms of Action

Molecules, 2018
Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE–Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography.
Wenxiao Jiao, Xiangxin Li, Handong Zhao, Jiankang Cao, Weibo Jiang   +4 more
doaj   +1 more source