Results 151 to 160 of about 169,356 (299)
Small, EarlyView.Recombinantly produced Tobacco Mosaic Virus coat protein (TMVcp) helical rods were demonstrated to reproducibly self‐assemble into hierarchically structured macroscopic filaments, driven by a lyotropic liquid crystal (LC) intermediate phase. The results indicate anisotropic molecular alignment of the fluid phase, retention of the native secondary ...
Guido M. Merino +2 more
wiley +1 more source
Proximity Proteomics Maps Candidate Cellular Uptake Pathways for Cell‐Derived Nanovesicles
Small Methods, EarlyView.TurboID‐based dual‐sided proteomics compares mechanically generated cell‐derived nanovesicles with naturally secreted small extracellular vesicles. Vesicle proteomes define candidate interaction signatures, while recipient‐cell proximity labelling identifies exposure‐associated proteins.
Eisuke Kanao +8 more
wiley +1 more source
British Journal of Pharmacology, EarlyView.Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz +8 more
wiley +1 more source
Purification and Properties of Extracellular Endoinulinase from Aspergillus niger 20 OSM
Food Technology and Biotechnology, 2006 Extracellular inulinase (E.C. 3.2.1.7) produced by Aspergillus niger 20 OSM culture in a 2-litre fermentor was isolated and purified by ion exchange chromatography on DEAE Sepharose, hydrophobic interaction on phenyl Sepharose, chromatofocusing on PBE-94,
Marcin Skowronek, Jan Fiedurek
doaj
, 2012 [目的]建立Q Sepharose FF分离藻红蛋白的工艺方法并验证其放大可行性。[方法]通过对洗脱条件、上样体积以及洗脱流速的优化,确定最佳分离工艺条件;在确定的工艺条件下,将上样体积与柱体积等比例放大。[结果]Q Sepharose FF分离藻红蛋白的最佳工艺条件为:将30ml坛紫菜提取液加载到8ml Q Sepharose FF柱上,以1ml/min的流速使用添加0-0.10-0.35-1.00mol/LNaCl的NaH2PO4-Na2HPO4缓冲溶液(pH6.0)分步洗脱,收集0.35mol ...
高敏 +5 more
core
Cancer Science, EarlyView.Adipocyte‐derived palmitic acid induces ZDHHC15‐mediated PPARγ S‐palmitoylation at Cys159, triggering its proteasomal degradation and promoting breast cancer malignancy with rosiglitazone resistance. Inhibition of PPARγ S‐palmitoylation by 2‐bromopalmitate suppresses breast cancer progression and synergistically enhances rosiglitazone efficacy ...
Geon Ho Moon +6 more
wiley +1 more source
TRIM59 Drives Bladder Cancer Progression Through E3 Ligase‐Dependent K48‐Linked Degradation of PTRF
Cancer Science, EarlyView.Schematic model of TRIM59‐driven BLCA progression through K48‐linked ubiquitination and proteasomal degradation of PTRF, leading to AKT activation and c‐Myc upregulation. ABSTRACT Tripartite motif‐containing 59 (TRIM59) is an E3 ubiquitin ligase implicated in multiple malignancies, but its role in bladder cancer (BLCA) remains incompletely understood ...
Junlin Gan +9 more
wiley +1 more source
, 2012 [目的]建立Q Sepharose FF分离藻红蛋白的工艺方法并验证其放大可行性。[方法]通过对洗脱条件、上样体积以及洗脱流速的优化,确定最佳分离工艺条件;在确定的工艺条件下,将上样体积与柱体积等比例放大。[结果]Q Sepharose FF分离藻红蛋白的最佳工艺条件为:将30 ml坛紫菜提取液加载到8 ml Q Sepharose FF柱上,以1 ml/min的流速使用添加0-0.10-0.35-1.00 mol/L NaCl的NaH2PO4-Na2HPO4缓冲溶液(pH 6.0)分步洗脱,收集0 ...
高敏 +5 more
core
The R203W substitution drives PACS‐1 syndrome by disrupting intramolecular regulation
The FEBS Journal, EarlyView.The middle region (MR) of PACS‐1 controls engagement with specific partner proteins. This manuscript presents the structure of the Furin binding region (FBR) and how interactions with partners are regulated through the interplay between a basic patch in the FBR and an acidic cluster in the MR.
Troy C. Krzysiak +7 more
wiley +1 more source
Binding of proteins to CNBr‐activated sepharose 4B [PDF]
FEBS Letters, 1974 David, Gary S. +2 more
openaire +2 more sources