Results 131 to 140 of about 169,356 (299)

Glycoengineered Recombinant Alpha1‐Antitrypsin Results in Comparable In Vitro and In Vivo Activities to Human Plasma‐Derived Protein

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Alpha‐1‐antitrypsin (A1AT) is a multifunctional, clinically important, high‐value therapeutic glycoprotein that can be used for the treatment of many diseases, such as A1AT deficiency, diabetes, graft‐versus‐host disease, cystic fibrosis, and various viral infections. Currently, the only U.S. food and drug administration‐approved treatment for
Frances Rocamora, Chen‐Lin Hsieh, Sanne Schoffelen, Johnny Arnsdorf, Eric A. Toth, Abdul S. Yunus, Thomas E. Cleveland IV, Sara Petersen Bjørn, Mina Ying Min Wu, Noel G. McElvaney, Bjørn Gunnar Rude Voldborg, Thomas R. Fuerst, Nathan E. Lewis   +12 more
wiley   +1 more source

IgE clonality and aggregation of insulin affect IgE-mediated activation of sensitized basophils

Journal of Allergy and Clinical Immunology: Global
Background: Skin prick testing and specific IgE determination do not discriminate between sensitization and clinically relevant insulin allergic reactions, whereas the basophil histamine release assay has proven useful in identifying clinically relevant ...
Steffen Christoffersen, MSc, Katrine Baumann, PhD, Anders Holm Millner, PhD, Henrik Toft-Hansen, PhD, Eva Johansson, PhD, Camilla Thorlaksen, PhD, Per Stahl Skov, PhD   +6 more
doaj   +1 more source

Isolation of carboxypeptidase N by affinity chromatography on column of CNBr-activated Sepharose with immobilized antibody

, 1979
The isolation of rat serum carboxypeptidase N (EC 3.4.2.2) by affinity chromatography on a column of CNBr-activated Sepharose with immobilized antibody is described.
Marie Petáková, Eva Simonianová
core   +1 more source

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

ISOLATION OF HUMAN-C4 BY AFFINITY CHROMATOGRAPHY WITH IGG-COUPLED SEPHAROSE 6B

, 1973
ISOLATION OF HUMAN-C4 BY AFFINITY CHROMATOGRAPHY WITH IGG-COUPLED SEPHAROSE ...
DH BING (18457356), Rebecca Patrick (13084221), J MERNITZ (18457353)   +2 more
core   +1 more source

A Simple and Efficient Method for Purification of Egg White Major Proteins Using Ion Exchange Chromatography

پزشکی بالینی ابن سینا, 2008
Introduction & Objective: Egg white contains four high-quantity proteins which have numerous applications. In this research, a simple and efficient method for the purification of those proteins was designed and performed based on ion exchange ...
Shamsi Veisi, Ali Mostafaie, Zahir Mohammad Hasan   +2 more
doaj  

Purification of the insulin peptide chains on phenyl sepharose column.

, 2018
The natively folded human insulin was purified using hydrophobic interaction chromatography (HIC) on a phenyl sepharose column. The elution was achieved with a reverse linear gradient of ammonium sulfate (500–0 mM) in 20 mM Tris HCl, pH 8.0. The standard
Reza Yousefi (3125688), Mohsen Akbarian (5873990)   +1 more
core   +1 more source

Ferritin as a Nanoparticle Scaffold for Plant‐Produced Next‐Generation Subunit Vaccines

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein nanoparticles offer an innovative approach to next‐generation subunit vaccine development by displaying antigenic sequences on the nanoparticle surface. Compared to traditional subunit vaccines, protein nanoparticle vaccines often show improved interaction with the immune system due to their particulate size and repetitive epitope ...
Jordan T. VanderBurgt, Richard Strasser, Christopher P. Garnham, Rima Menassa   +3 more
wiley   +1 more source

Sepharose-conjugated SRL induced growth inhibition of breast cancer cells.

, 2014
(A) SRL shows strong binding to MCF-7 cells and very weak binding to (B) HMEC. (C) MCF-7 cells were incubated without or with SRL or Sepharose-SRL (C-SRL), at equivalent concentrations to SRL with 128 and 256 hemagglutination units (HAU) activity for 72 ...
Sachin M. Eligar (484719), Pravin Mahajan (653802), Bale M. Swamy (484723), Lu-Gang Yu (394218), Anita Borges (653803), Arvind. Ingle (653804), Mohammed Azharuddin Savanur (653801), Radha Pujari (484718), Padma Shastry (76153), Jonathan M. Rhodes (394217), Chen Chen (6544), Rajiv D. Kalraiya (653805), Shashikala R. Inamdar (484724)   +12 more
core   +1 more source

Pt‐TiO2 mesoporous nanosystem co‐delivering phlorezin enables synergistic sonodynamic‐chemoimmunotherapy for hepatocellular carcinoma via directly targeting DDX5 and metabolic reprogramming modulation

BMEMat, EarlyView.
Abstract Hepatocellular carcinoma (HCC), ranking as the third leading cause of cancer‐related mortality globally, continues to pose significant therapeutic challenges. Here, we developed an innovative nanosystem, Phl@PT, based on Pt‐TiO2 nanoparticles for the co‐delivery of Phlorezin, presenting a novel approach for HCC treatment through sonodynamic ...
Kairui Liu, Bo Zhang, Yue Wan, Zhiru Yang, Abdusami Abdurahman, Mengxin He, Shengtao Hu, Yi Zhang, Xiaoke Li, Jin Chang, Jun Kang, Liren Liu, Wanghua Liu, Shao Li   +13 more
wiley   +1 more source