Results 121 to 130 of about 169,356 (299)

Characterization of the Chemical and Functional Stability of DEAE Sepharose Fast Flow

open access: yes, 1993
The release of amines from the ion-exchange groups in DEAE Sepharose® Fast Flow has been studied under static and column conditions. The leakage compounds have been identified and quantified by gas chromatography—mass spectrometry.
Andersson, Mikael   +2 more
core   +1 more source

Synthese und aktivitätsbasierte Proteinprofilierung identifizieren die Aldo‐Keto‐Reduktase 1C3 als Zielprotein der Myxoglucamide

open access: yesAngewandte Chemie, EarlyView.
Myxoglucamid A – ein neuartiges, aus Myxobakterien isoliertes Glykolipopeptid – wurde erstmals totalsynthetisch hergestellt. Mittels aktivitätsbasierter Proteinprofilierung (ABPP) wurde die Aldo‐Keto‐Reduktase 1C3 (AKR1C3) als primäres Zielprotein in menschlichen Zellen identifiziert.
Thomas Siemon   +9 more
wiley   +1 more source

Ganglioside-Sepharose 4B 에 의한 human fibroblast interferon 의 정제

open access: yes, 1982
학위논문(석사) - 한국과학기술원 : 생물공학과, 1982.2, [ x, 77 p. ]The cell surface is important in several phases of interferon action. When a cell is treated with an interferon to which it is sensitive, an antiviral state develops.
Chung, Hong-Suek, 정홍석
core   +1 more source

Covalent immobilization of cyclodextrin glucosyltransferase (CGTase) in activated silica and Sepharose

open access: yes, 2002
229-234Cyclodextrin glucanotransferase is a non-Leloir glycosyltransferase that directly employs the free energy of cleavage of starch to produce cyclodextrins. In presence of appropriate acceptors, this enzyme synthesizes oligosaccharides containing α(l→
Ballesteros, Antonio   +3 more
core  

Sequential affinity chromatography of sIgA-2 and sIgA-3 preparations on casein-Sepharose and lipid-resin.

open access: yes, 2013
After chromatography of sIgA-2 and sIgA-3 preparations on ATP-Sepharose (Figs 3C and 3D, respectively), fractions 10–18 having high affinity to ATP-Sepharose were combined and chromatographed on casein-Sepharose (Figs 4A and 4B, respectively).
Valentina N. Buneva (300752)   +2 more
core   +1 more source

Affinity chromatographic separation of arylsulfatase A and B using cibacron blue-sepharose

open access: yes, 1977
A simple and rapid affinity chromatographic method is described using Cibacron Blue F3GA-Sepharose for the separation of arylsulfatases A and B (aryl-sulfate sulfohydrolase, E C 3.1.6.1)
Ahmad, Ateeq   +2 more
core   +1 more source

Synthesis and Activity‐Based Protein Profiling Identifies Aldo‐Keto Reductase 1C3 as Target Protein of Myxoglucamides

open access: yesAngewandte Chemie International Edition, EarlyView.
Myxoglucamide A—a novel glycolipopeptide isolated from myxobacteria—was synthesized, and activity‐based protein profiling identified aldo‐keto reductase 1C3 (AKR1C3) as its main target in human cells. Structure‐activity relationship studies, together with biophysical characterization and X‐ray crystallography, elucidated the structural basis of AKR1C3 ...
Thomas Siemon   +9 more
wiley   +1 more source

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

open access: yesBiotechnology and Bioengineering, EarlyView.
One‐pot liquid‐phase synthesis of the synthetic ligand B1Al2A2 enables controllable ligand density and efficient capture of IgG and IgY with high recovery and purity, streamlining small‐ligand affinity purification workflows. ABSTRACT The development of synthetic affinity ligands offers a cost‐effective alternative to traditional biological ligands ...
Carolina Mota Natal   +8 more
wiley   +1 more source

A new sepharose derivative containing covalently bound myo-inositol: Its structure and application

open access: yes, 1979
myo-Inositol was covalently bound on, an epoxy-activated sepharose. In order to elucidate the structure of the derivative of sepharose formed, in a model reaction myo-inositol was coupled to propylene oxide, whereby two compounds were obtained.
Breitenbach, Michael, Fazldeen, Hussan
core   +1 more source

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