Results 101 to 110 of about 169,356 (299)
Molecules, 2017 A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li +5 more
doaj +1 more source
Coupling Antibody to Cyanogen Bromide-Activated Sepharose [PDF]
, 2014 Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.
Jennifer M, Kavran, Daniel J, Leahy
openaire +2 more sources
Ligand screening assay for the enzyme kallikrein immobilized on NHS-activated Sepharose
, 2021 Human tissue kallikreins (KLKs) are serine proteases involved in various physiological and pathological conditions, including cancer and neurological disorders. These enzymes constitute attractive drug targets, which has stimulated the search for new KLK
Ximenes, Valdecir Farias [UNESP] +3 more
core +1 more source
α‐Synuclein Forms Distinct Micelle‐Like Assemblies at Low Ionic Strengths
Advanced Science, EarlyView.At high ionic strength, α‐synuclein forms diverse assemblies, including oligomers, fibrils, and condensates. Here, we show that at low ionic strength, α‐synuclein adopts a distinct, low‐abundance assembly state. These assemblies maintain a constant size above a critical concentration and do not coalesce, suggesting a micelle‐like organization ...
Sophie Hertel +10 more
wiley +1 more source
Angewandte Chemie, EarlyView.A modular farnesyltransferase‐based chemoenzymatic platform enables fast, site‐specific labeling of nanobodies for PET imaging of inflammation. CD11b‐targeted PET agents selectively image myeloid‐driven inflammation, while CD45‐targeted PET agents provide sensitive whole‐body immune mapping.
Sneha Venkatachalapathy +7 more
wiley +2 more sources
The fractionation of t-RNA on N,N′-bis(3-aminopropyl)-piperazine substituted-Sepharose
, 1974 An anion exchange agarose has been prepared by modifying sepharose 6B with N, N′-bis (-3-aminopropyl) piperazine. This material (BAPP-Sepharose) has been used for the fractionation of t-RNA from E.coli by column chromatography.
Acosta, Z. +5 more
core +1 more source
Advanced Science, EarlyView.The Tuta absoluta salivary effector TaCRVP subverts tomato immunity by hijacking host catalase SlCAT2. Through a sophisticated dual mechanism, TaCRVP simultaneously promotes SlCAT2 tetramerization for activation and blocks its SlAdBiL‐mediated degradation for stabilization.
Jianquan Yan +9 more
wiley +1 more source
Identification of an adeno-associated virus binding epitope for AVB sepharose affinity resin
Molecular Therapy: Methods & Clinical Development, 2015 Recent successes of adeno-associated virus (AAV)âbased gene therapy have created a demand for large-scale AAV vector manufacturing and purification techniques for use in clinical trials and beyond.
Qiang Wang +5 more
doaj +1 more source
Properties of Sepharose-Bound beta-Lactamase from Enterobacter Cloacae
, 1985 beta-Lactamase from Enterobacter cloacae P99 was immobilized onto Sepharose by the cyanogen bromide activation method and the properties of the Sepharose-bound enzyme were compared with those of soluble and cell-bound enzyme.
DALZOPPO, DANIELE +2 more
core
Advanced Science, EarlyView.Corynoxine exerts potent broad‐spectrum anticancer activity by directly targeting NQO1. This interaction dissociates the oncogenic NQO1‐PTPA complex, releasing PTPA to robustly activate the tumor suppressor PP2A. Consequently, downstream Raf/MEK/ERK and PI3K/AKT signaling pathways are suppressed, downregulating c‐Myc and driving tumor regression ...
Guoqing Hou +6 more
wiley +1 more source