Results 81 to 90 of about 169,356 (299)

Affinity chromatography of galactose containing biopolymers using covalently coupled Ricinus communis lectin to sepharose 4B

open access: yes, 1975
A galactose-specific lectin isolated from Ricinus communis beans has been covalently coupled to Sepharose 4B activated with cyanogen bromide. The immonolized lectin retains its polysaccharide-binding property.
Ahmad, Ateeq   +2 more
core   +1 more source

PolyG Fibrils Coalesce Into Nuclear Ribbons That Engage Proteostasis Machinery in Neuronal Intranuclear Inclusion Disease

open access: yesAdvanced Science, EarlyView.
In NIID, expanded NOTCH2NLC repeats give rise to nuclear polyG inclusions. Tracer‐guided in situ cryo‐electron tomography enables cross‐scale structural analysis from mouse brain to native neuronal nuclei, revealing dense‐core/peripheral‐halo inclusions built from compact polyG ribbons.
Hui Dong   +13 more
wiley   +1 more source

Subfractionation of human very low density lipoproteins by heparin-Sepharose affinity chromatography

open access: yesJournal of Lipid Research, 1983
Very low density lipoproteins obtained from normolipidemic subjects were fractionated into subclasses by means of affinity chromatography on a heparin-Sepharose column in the presence of MnCl2. The four subfractions eluted at 0.05, 0.12, 0.20, and 0.38 M
E Trezzi, C Calvi, P Roma, A L Catapano
doaj   +1 more source

Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil

open access: yesMolecules, 2020
Functional properties of each enzyme strictly depend on immobilization protocol used for linking enzyme and carrier. Different strategies were applied to prepare the immobilized derivatives of Rhizomucor miehei lipase (RML) and chemically aminated RML ...
Maryam Yousefi   +5 more
doaj   +1 more source

Purification of PHB depolymerase of Stenotrophomonas sp. RZS7 on Octyl sepharose column.

open access: yes, 2020
Purification of PHB depolymerase of Stenotrophomonas sp. RZS7 on Octyl sepharose column.
Hesham Ali El-Enshasy (8259831)   +4 more
core   +1 more source

Herbicide Metabolic Resistance in Poaceae Plants via the GA‐GID1/DELLA‐DOF2‐P450s Module

open access: yesAdvanced Science, EarlyView.
Plant hormone signaling modulates herbicide resistance in Echinochloa crus‐galli. The GA–DELLA–DOF2 cascade directly activates P450 detoxification genes in two Poaceae species, uncovering a core mechanism of metabolic herbicide resistance. ABSTRACT Barnyard grass (Echinochloa crus‐galli) is one of the world's most important weeds, and the evolution of ...
Junzhi Wang   +6 more
wiley   +1 more source

Miro1 Protects Against Acute Kidney Injury Through Modulating Mitochondrial Homeostasis via Interaction With Sirt6

open access: yesAdvanced Science, EarlyView.
Ischemia‐reperfusion reduces Sirt6 activity, thereby increasing Miro1 acetylation. Hyperacetylated Miro1 exhibits perinuclear distribution and degradation, thereby inducing mitochondrial dysfunction and promoting apoptosis in renal tubular epithelial cells. This pathway reveals a mechanistic link between Sirt6‐mediated deacetylation and Miro1 stability
Lin Wu   +12 more
wiley   +1 more source

Entamoeba histolytica: detection of coproantigens by purified antibody in the capture sandwich ELISA Entamoeba histolytica: detecção de coproantígenos por ELISA de captura utilizando anticorpo purificado

open access: yesRevista do Instituto de Medicina Tropical de São Paulo, 1994
A sensitive and specific Capture Sandwich ELISA (CSE) was developed using polyclonal purified rabbit antibodies against three different axenic strains of Entamoeba histolytica: CSP from Brazil and HM1 - IMSS from Mexico, for the detection of ...
Haidee Urdaneta   +3 more
doaj   +1 more source

ATL2 Recruits TRAK1 to Promote Mitochondrial Transport at ER–Mitochondria Contact Sites

open access: yesAdvanced Science, EarlyView.
ABSTRACT Mitochondrial transport and distribution are crucial for cellular homeostasis, yet whether and how they are regulated by endoplasmic reticulum (ER)–mitochondria contact sites remains unclear. Here, we demonstrate that the ER protein atlastin‐2 (ATL2) orchestrates mitochondrial transport and distribution by promoting assembly of the transport ...
Yiru Cheng   +9 more
wiley   +1 more source

Evaluation of the performance of affinity matrix produced from improved variants of S3 peptide to remove endotoxin from Active pharmaceutical ingredient of streptokinase and comparison with commercial matrices [PDF]

open access: yesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
Introduction:  Endotoxin or lipopolysaccharide (LPS) is one of the components of the wall of gram-negative bacteria, which, when in contact with blood, stimulates the immune system and causes fever and even serious adverse effects or death.
Shahin Hadadian   +2 more
doaj  

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