Results 71 to 80 of about 169,356 (299)

Expression of linoleate isomerase gene from Lactobacillus reuteri PYR8 in Pichia pastoris

浙江大学学报. 农业与生命科学版, 2006
Linoleate isomerase gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8, then the gene was cloned into Pichia pastoris expression vector pPIC9K. The recombinant plasmid pPIC9K-LI was transformed into P.
ZHANG Yan-he, ZHANG Lan-wei, HU Sen, BU Zhi-gao   +3 more
doaj   +1 more source

MAM‐Localized MANF Counteracts Microinflammatory Stress to Attenuate Mitochondrial Dysfunction and Cataractogenesis in High Myopia

Advanced Science, EarlyView.
Chronic microinflammation in high myopia suppresses MANF in lens epithelial cells. MANF normally resides within MAMs and promotes ubiquitin‐mediated degradation of the ER Ca2+ pump SERCA2. MANF loss causes pathological SERCA2 accumulation, MAM hyperassembly, and disrupted ER‐to‐mitochondria Ca2+ transfer, leading to mitochondrial failure, oxidative ...
Xin Liu, Hao Li, Ching Kang, Dongling Guo, Shuyu Liu, Jiaqi Meng, Zhiqian Huang, Yu Du, Xiangjia Zhu   +8 more
wiley   +1 more source

LC-MS chromatograms of IgG sepharose-binding molecules.

, 2014
A. Representative LC-MS chromatograms of a IgG sepharose-binding molecule (m/z 1414.3) are shown in a KD patient and a DC control. TIC: Total ion current chromatograms, XIC: Extracted-ion chromatograms at m/z 1400–1500, and extracted-ion chromatograms at
Hisanori Nishio (663950), Mitsumasa Saito (663951), Yasunari Sakai (663954), Kenji Murata (500161), Shunsuke Kanno (663949), Kenichiro Yamamura (663953), Toshiro Hara (172890), Tomofumi Miyamoto (663956), Yumi Mizuno (282050), Kazunobu Ouchi (663957), Takeshi Kusuda (663947), Tamami Tanaka (663952), Hidetoshi Takada (663955), Yasutaka Nakashima (663948), Kenji Waki (663958)   +14 more
core   +1 more source

Porcine Deltacoronavirus M Protein Binds NLRP3 to Promote Inflammasome Assembly via Competition with TRIM31

Advanced Science, EarlyView.
Porcine deltacoronavirus (PDCoV) infection induces severe intestinal inflammation and acute diarrhea in piglets, yet the molecular mechanism remains incompletely understood. The M protein activates NLRP3 inflammasome through dual mechanisms: direct binding to the NLRP3 LRR domain and disruption of TRIM31‐mediated K48‐linked ubiquitination.
Jinhui Hou, Fangfang Han, Anqi Liu, Yang Yu, Rui Zhao, Zhengting Shi, Yanrong Lv, Jinli Liu, Shaopo Zu, Zhanyong Wei, Jin Yuan, Hui Hu   +11 more
wiley   +1 more source

Protease activity of protein captured by TcCYS3 and TcCYS4 coupled to CNBr–Sepharose.

, 2016
Qualitative assay to test protease activity of samples obtained by capturing system. a SDS-PAGE gel showing bands of protease activity of about 30 and 45 kDa (arrows). b IEF gel showing clear bands of protease activity at about pH 4.0.
Fabienne Micheli (317194), Fátima Cerqueira Alvim (524172), Ana Camila Oliveira Freitas (716197), Thyago Hermylly Santana Cardoso (317192), Bruno Silva Andrade (834403), André da Silva Santiago (524171), Karina Peres Gramacho (834405), Carlos Priminho Pirovani (317193), Daniela Martins Koop (834404), Aurizangela Oliveira de Sousa (2198419)   +9 more
core   +1 more source

SiDT1 Defines Plant Architecture Reminiscent of Green Revolution in Foxtail Millet

Advanced Science, EarlyView.
SiDT1 encodes a GA3‐oxidase that creates a semi‐dwarf, lodging‐resistant architecture reminiscent of the rice Green Revolution. The resulting ideotype performs well under dense planting and provides a valuable genetic resource for high‐yield, mechanized foxtail millet production. ABSTRACT Foxtail millet (Setaria italica) is a drought‐tolerant C4 cereal
Jianzhen Lv, Jinjin Cheng, Zhen Hu, Qian Lan, Yanjun Yang, Chengcheng Pei, Junhao Luo, Zhuang Li, Peiyong Xin, Jijun Yan, Jinfang Chu, Qian Qian, Zhaosheng Kong, Liang Jiang   +13 more
wiley   +1 more source

Analysis of toxoplasma gondii proteins after Triton X-114 solubilization and hidropholic chromotograhy Análise de proteínas do Toxoplasma gondii após solubilização com Triton X-114 e cromatografia hidrofóbica

Memorias do Instituto Oswaldo Cruz, 1988
The distribution of the surface proteins of toxoplasma gondii radiodinated were studied using the phase separation technique and ability of binding in the phenyl-Sepharose column.
Salvatore Giovanni De Simone, Helena C. B. Guedes, Izidro Bendet   +2 more
doaj   +1 more source

Pseudo-affinity chromatography of rumen microbial cellulase on Sepharose- Cibacron Blue F3GA [PDF]

, 2013
Pseudo affinity adsorption of bioproducts on Sepharose-cibacron blue F3-GA was subjected to rumen microbial enzyme evaluation through batch binding and column chromatography of cellulase. The results showed that homogenizing method had better performance
Panahi, HA, Rasi, HM, Sadeghi, AA, Chamani, M   +3 more
core   +1 more source

Subversion of Atypical Mucin Traps by a Spore‐Coat Effector Blocks Cellular Immunity in Drosophila

Advanced Science, EarlyView.
While the entomopathogenic fungus Metarhizium robertsii can evade insect humoral immunity, its subversion of cellular defenses is still elusive. We identify Eac1 as a spore‐coat protein essential for infecting drosophilids by targeting atypical mucins (Sgf1 and Sgf2), which can entrap spores in a gel matrix and thereby facilitate hemocyte encapsulation.
Shiqin Li, Haimin Chen, Gangqi Fang, Dongxiang Wei, Hongyun Wu, Chen Chen, Song Hong, Chengshu Wang   +7 more
wiley   +1 more source

Affinity chromatography of creatine phosphokinase on organomercurial‐sepharose [PDF]

FEBS Letters, 1975
Creatine phosphokinase (CPK, EC 2.7.3.2) occurs mainly in muscle where it catalyses the reversible transfer of phosphate between ADP and creatine phosphate [ 1 ] . The enzyme has been isolated by combining alcohol and salt fractionation [ 1 ] followed by DEAE-column chromatography [2,3] .
openaire   +2 more sources