Results 91 to 100 of about 169,356 (299)
Covalently Linked Peptides and Membrane Potential Enable CyaA Segment Translocation
Advanced Science, EarlyView.Using a newly developed Droplet Interface Bilayer‐Pipette assay, the authors show that two segments of the CyaA toxin cross membranes by distinct mechanisms. One segment translocates spontaneously, whereas the translocation of the other depends on membrane potential.
Gaia Scilironi +9 more
wiley +1 more source
Molecules, 2017 The oleaginous yeast Moniliella spathulata R25L270 was the first yeast able to grow and produce extracellular lipase using Macaúba (Acrocomia aculeate) cake as substrate.
Lívia T. de A. Souza +6 more
doaj +1 more source
Selective Modulation of OTUB1 Noncanonical Function via a bioPhosTAC Strategy
Advanced Science, EarlyView.This work positions the versatile performance of the peptide‐based bioPhosTAC platform for dissecting phosphorylation‐dependent biology and expanding the scope of induced‐proximity technologies. We demonstrated that selective manipulation of a tyrosine phosphorylation site is sufficient to propagate coordinated cellular consequences.
Seung Un Seo +7 more
wiley +1 more source
Biosaintifika: Journal of Biology & Biology Education, 2018 Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced
Teuku M. Iqbalsyah +2 more
doaj +1 more source
Rac1 mutants interact with CaM-Sepharose.
, 2012 (A) HA-Rac1 WT and HA-Rac1 mutants or (B) GFP-Rac1 wild type, GFP-Rac1(Q61L) and GFP-Rac1(T17N) were expressed in HeLa cells. CaM-Sepharose 4B beads (80 µl) were incubated with HeLa cell lysate (200 μg) expressing various forms of HA-Rac1 or GFP-Rac1 for
Rajinder P. Bhullar (142507) +2 more
core +1 more source
OsCPK12‐OsATPD1 Module Regulates Photosynthetic Acclimation to Light Stress in Rice
Advanced Science, EarlyView.OsCPK12 interacts and phosphorylates OsAtpD1 at Ser8 in its transit peptide. This phosphorylation promote its chloroplast translocation, thereby maintaining ATP synthase activity and proton gradient (ΔpH) homeostasis and regulating photosynthetic acclimation, photoprotection, and repair under light stress.
Beifang Wang +17 more
wiley +1 more source
Purification of bovine pancreatic glucagon as a by-product of insulin production
Brazilian Journal of Medical and Biological Research, 1997 A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation.
A.S.R. Andrade, L. Vilela, H. Tunes
doaj +1 more source
RSF1‐Dependent PAR Turnover Promotes 53BP1 Liquid Condensate Formation at DNA Damage Sites
Advanced Science, EarlyView.At sites of DNA damage, RSF1 recruits PARG to accelerate PAR turnover, triggering a switch from PAR‐driven condensates to 53BP1 condensates. This condensate transition enables p53‐dependent gene transcription and coordinates the DNA damage response.
Yungyeong Heo +10 more
wiley +1 more source
Angewandte Chemie, EarlyView.We present a foldamer strategy to downsize the histone H3‐H4 quaternary structure into high‐affinity, medium‐sized binders (∼2 kDa) for the histone chaperone ASF1. Our peptide‐oligourea chimeras, designed through structure‐guided optimization and foldamer chemistry, recapitulate the natural H3‐H4 dimer binding mode while demonstrating plasma stability ...
Bo Li +15 more
wiley +2 more sources
On the utility of immobilized phenylarsine oxide in the study of redox sensitive cardiac proteins
Scientific ReportsReactive protein cysteine thiols are critical to sensing and transducing oxidant signals, often by induction of disulfide bonds that alter their activity or interactions.
Asvi Arora Francois +5 more
doaj +1 more source