Results 111 to 120 of about 169,356 (299)

Purificación de la proteína banda 3 de eritrocitos humanos (AE 1) Purification of band 3 protein from human erythrocytes (AE 1)

Revista Cubana de Hematología, Inmunología y Hemoterapia, 2007
Se purifica la proteína banda 3 a partir de membranas de eritrocitos humanos con rapidez y eficiencia, mediante una combinación de cromatografía de intercambio iónico en DEAE-celulosa y de afinidad mediante el empleo de una columna pCMB-Sepharose.
Ada Amalia Arce Hernández, Rinaldo Villaescusa Blanco, Julio César Merlín Linares   +2 more
doaj  

GSTM4 Suppresses Prostate Cancer Progression by Targeting TRIM27‐mediated ACLY Ubiquitination and Lipid Metabolism

Advanced Science, EarlyView.
GSTM4 enhances the interaction between ACLY and the E3 ubiquitin ligase TRIM27, thereby increasing K48‐linked ubiquitination at the K554 site of ACLY and promoting its degradation. Consequently, GSTM4 regulates lipid synthesis, as well as the proliferation and metastasis of PCa cells, by modulating ACLY stability.
Kang‐Ping Xiong, Fei Liu, Wang Wang, Gang Wang, Kai‐Yu Qian, Sheng Tu, Si‐Ming Chen, Hui‐Min Xu, Shan‐Shan Zhang, Yong Li, Xing‐Huan Wang, Hong Weng   +11 more
wiley   +1 more source

Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase

Memorias do Instituto Oswaldo Cruz, 1998
The enzyme triosephosphate isomerase (TPI, EC 5.3.1.1) was purified from extracts of epimastigote forms of Trypanosoma cruzi. The purification steps included: hydrophobic interaction chromatography on phenyl-Sepharose, CM-Sepharose, and high performance ...
Bourguignon SC, Meirelles MN, Pacheco RS, De Simone S Giovanni   +3 more
doaj  

Characterization of a corticosteroid 21-dehydroxylase from the intestinal anaerobic bacterium, Eubacterium lentum.

Journal of Lipid Research, 1980
An oxygen-sensitive corticosteroid 21-dehydroxylase has been characterized in cell extracts of Eubacterium lentum. The enzyme was highly specific for corticosteroids containing and alpha-ketol structure and required FMNH2 or reduced benzyl viologen for ...
S D Feighner, P B Hylemon
doaj   +1 more source

Macrophage RSAD2 Couples mtDNA Synthesis With a Self‐Amplifying Inflammatory Circuit to Orchestrate Tissue Repair

Advanced Science, EarlyView.
Macrophage RSAD2 dually regulates CMPK2—the rate‐limiting enzyme for mitochondrial DNA synthesis—by suppressing its ubiquitination and promoting its phosphorylation via Csnk2a2 recruitment. This amplifies mtDNA production, which simultaneously activates a cGAS‐STING‐IRF3‐RSAD2 feedforward loop and the NLRP3 inflammasome, driving a self‐sustaining ...
Haomiao Yuan, Shukui Du, Chunfei Li, Xinjie Li, Shuyang Mu, Anran Qu, Hengxu Yan, Chongchong Xu, Jinnong Yang, Jingkai Sun, Yijun Wang, Jiayang Xiao, Fuyuan Zhang, Linlin Wang, Tiegang Li, Rui Zhao, Dawei Guan   +16 more
wiley   +1 more source

An Unbiased Geometry‐Resolved Membrane Platform for Proteome‐Scale Discovery of Membrane Curvature Sensors

Advanced Science, EarlyView.
A novel geometry‐resolved membrane platform provides a seamlessly integrated pipeline for studying curvature‐sensing proteins. By linking shotgun proteomics with quantitative imaging, this approach enables both the unbiased discovery and rigorous validation of novel sensors across the entire curvature range—positive, negative, and zero.
Takumi Komikawa, Rikuto Kawakami, Kunanon Chattrairat, Tatsuya Niwa, Taiga Ajiri, Takao Yasui, Masayoshi Tanaka   +6 more
wiley   +1 more source

Lytic antibodies elicited by Trypanosoma cruzi infection recognize epitopes present on both bloodstream trypomastigote and epimastigote forms of parasite

Revista do Instituto de Medicina Tropical de São Paulo, 1988
Sera of Chaga's disease patients containing anti-T. cruzi lytic antibodies were submitted to affinity chromatography using Sepharose 4B conjugated with antigen extracted from epimasiigote or trypomasiigote forms of the parasite.
Harumi A. Takehara, Diva F. Cardoso, Ana M.M. da Silva, I. Mota   +3 more
doaj   +1 more source

Study of the feasibility of separation of carrier ampholytes from peptides by hydrophobic interaction chromatography on octyl-sepharose [PDF]

, 1980
The merits of hydrophobic interaction chromatography on octyl-Sepharose as a method for the separation of carrier ampholytes from peptides have been investigated.
Gelsema, W.J., Roozen, A.M.P., W.J. Gelsema, Hamer, R.J., W.M. Blanken, C.L. De Ligny, R.J. Hamer, Blanken, W.M., Ligny, C.L. de, A.M.P. Roozen, J.A. Bakker   +10 more
core   +1 more source

Palmitoyl Acyltransferase Zdhhc17 Promotes Functional Recovery After Spinal Cord Injury by Targeting the Nuclear Transport Factors Kpna2 and Ipo9

Advanced Science, EarlyView.
In this study, we demonstrate that Zdhhc17, acting as a PAT, suppresses neuronal apoptosis and promotes axon regeneration after injury, thereby enhancing functional recovery following SCI. In this context, Kpna2 and Ipo9 serve as novel palmitoylation substrates of Zdhhc17, whose palmitoylation prevents the injury‐induced degradation of their proteins ...
Meixuan Chen, Huan Chen, Sining Ding, Fan Liu, Xiaofei Zheng, Yuan Wang, Ruitu Tian, Huan Li, Peilin Liu, Le Hu, Bin Liu, Limin Rong, Mangmang Li   +12 more
wiley   +1 more source

Distribution of Big Tau Isoforms in the Human Central and Peripheral Nervous System

Annals of Neurology, EarlyView.
Objective Tau is widely studied in neurodegeneration, yet most work has focused on canonical brain tau isoforms. A longer isoform, “big tau,” produced by inclusion of exon 4a, is expressed in the peripheral nervous system (PNS) and central nervous system (CNS) regions.
Rama Krishna Koppisetti, Nicolas R. Barthélemy, Kanta Horie, Cindy V. Ly, Kaleigh F. Roberts, Srinivas Koutarapu, Richard J. Perrin, Erin E. Franklin, Chiara Pedicone, Joshua Orrick, Justin Melendez, Timothy M. Miller, Chihiro Sato, Nupur Ghoshal, Alison M. Goate, Celeste M. Karch, Randall J. Bateman, Soumya Mukherjee   +17 more
wiley   +1 more source