Results 61 to 70 of about 169,356 (299)

Pericentrosomal Redistribution of the Endoplasmic Reticulum Ensures Organelle Symmetric Inheritance and Mitotic Progression

Advanced Science, EarlyView.
Upon mitotic entry, RTN4 relocalizes to the pericentrosomal region, forming a more tubular ER network around centrosomes. CDK1‐mediated phosphorylation of RTN4 increases its interaction with Rab11 GTPase, facilitating dynein‐dependent transport of RTN4 to the pericentrosomal region.
Xiangyu Xu, Yalin Liu, Rongyi Wang, Wenwen Xu, Hao Shi, Ning Huang, Junlin Teng, Jin Meng, Pengli Zheng, Jianguo Chen   +9 more
wiley   +1 more source

Neuron‐Derived MIF Engages VCAM1 to Fuel a Self‐Amplifying CXCL8 Loop That Drives Perineural Invasion and Metastasis in Gastric Cancer

Advanced Science, EarlyView.
Neuron‐derived MIF binds VCAM1 on gastric cancer cells and activates ERK/STAT3 signaling, leading to CXCL8 transcription and secretion. Tumor‐derived CXCL8 subsequently stimulates neuronal CXCR2 to enhance MIF production, establishing a self‐amplifying MIF–VCAM1–CXCL8 positive‐feedback loop that promotes perineural invasion, tumor progression, and ...
Xunjun Li, Zhongya Zhai, Haiyi Yu, Renjie Qiu, Fengyu Li, Luxi Xiao, Boxi Huang, Huiying Liu, Jiayong He, Bowen Cai, Jiawen Chen, Jiang Yu, Guoxin Li, Tao Chen   +13 more
wiley   +1 more source

Subfractionation of human high density lipoproteins by heparin-Sepharose affinity chromatography.

Journal of Lipid Research, 1980
A reproducible and quantitative subfractionation of human high density lipoproteins (HDL) by heparin-Sepharose affinity chromatography has been developed. Two elution methods (A and B) were used to subfractionate HDL(2) (d 1.063-1.125 g/ml) or total HDL (
K H Weisgraber, R W Mahley
doaj   +1 more source

Spectral properties and chromophore rotation of phytochrome bound to substituted Sepharose

, 1986
Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose.
Sundqvist, C, Sundqvist, Christer, Ekelund, Nils,   +2 more
core   +1 more source

Anticorpos anti-HBs obtidos em coelhos e sua utilização como reagentes para testes sorológicos no diagnóstico da hepatite B Anti-HBs antibodies obtained in rabbits and its use as reagents for serological tests in the diagnosis of hepatitis B

Memorias do Instituto Oswaldo Cruz, 1984
Pela técnica de cromatografia de afinidade, utilizando-se a resina de Sepharose 4B ligada ao anti-HBs, obteve-se na passagem de plasma de portador assintomático de antígeno HBs, um antígeno parcialmente purificado.
C. F. T. Yoshida, L. A. C. Mercadante, A. M. C. Gaspar, M. M. S. Maia, O. J. Berro, E. C. C. de Almeida, H. G. Schatzmayr   +6 more
doaj   +1 more source

One step purification of AK-TNFα via Ap5A affinity elution off Blue-Sepharose chromatography.

, 2016
(A) SDS-PAGE analysis of AK-TNFα purification. M: Protein-molecular-weight size markers. Lane 1: Cleared soluble bacterial cell lysate. Lane 2: Flow-through fraction from Blue-Sepharose column. Lane 3: Ap5A elution of AK-TNFα from Blue-Sepharose.
Xinyu Liu (34266), Joshua G. Liang (2805232), Xinxin Liu (534717), Dan Luo (192943), Peng Liang (491715), Rongchuan Zhao (2805229), Caixia Wen (2805226), Jingjing Cui (1711807)   +7 more
core   +1 more source

ZER1 Restrains Pressure Overload‐Induced Cardiac Remodeling by Targeting DVL2 for Gly/N‐Degron‐Dependent Degradation

Advanced Science, EarlyView.
Pressure overload suppresses cardiomyocyte ZER1, weakening CRL2Zer1‐mediated DVL2 degradation and allowing DVL2 accumulation. Elevated DVL2 activates CaMKII‐HDAC4‐MEF2C signaling, drives fetal gene reactivation, and promotes pathological remodeling.
Mingchao Jiang, Zhehao Lin, Lu Chen, Ying Ni, Jun Zhou, Wenjuan Zhang, Huilin Wang, Peifeng Ying, Xiu Lu, Kai Wang, Qingran Kong, Naxin Xu, Dingsheng Zhao, Jianwei Li, Guohui Zhong, Xingchen Meng, Sarkawt Hamad, Junmeng Zheng, Yuan Fu, Rongjiang Qin, Xuran Chu, Shangxuan Li, Youyou Li, Yili Wu, Yi Wang, Weihong Song, Yingxian Li, Shukuan Ling   +27 more
wiley   +1 more source

PURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L. [PDF]

Journal of Sciences, Islamic Republic of Iran, 2002
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-
doaj  

Comparison of Sepharose resins.

, 2018
(A) Comparison between resin made with 12-ADA CaM from clarified lysate (12-ADA CaM Lysate Seph) and Calmodulin Sepharose 4B (GE CaM Seph) (n = 12) ** = p≤0.01.
Julia G. Fraseur (1504747), Tamara L. Kinzer-Ursem (1504738)   +1 more
core   +1 more source

The E3 Ubiquitin Ligase RLIM Safeguards Oligodendrocyte Development and Myelination by Targeting SLC7A11 for Polyubiquitination to Regulate Ferroptotic Resistance

Advanced Science, EarlyView.
RLIM‐mediated SLC7A11 polyubiquitination is required for SPTBN2‐mediated trafficking and membrane localization of SLC7A11, which enables oligodendrocyte precursor cells to be resistant to ferroptosis and safeguards oligodendrocyte lineage progression and thus myelination.
Yuwei Li, Haijun Zhang, Jieya Zhou, Shiyu Yang, Jingmei Cha, Gaofeng Yan, Shuhua Zhao, Bingyu Mao, Pengcheng Ma   +8 more
wiley   +1 more source