Results 41 to 50 of about 169,356 (299)

Affinity isolation of RNA polymerase II on amanitin-Sepharose [PDF]

, 1984
We report here the first case of an affinity isolation of eukaryotic RNA polymerase II. The procedure employs an affinity matrix composed of [alpha]-amanitin coupled to Sepharose 4B via a ten atom spacer.
Faulstich, H., Faulstich, Heinz, Lutter , L., Lutter, Leonard C.   +3 more
core   +1 more source

Western blotting analysis of biotinylated proteins using streptavidin sepharose.

, 2022
Cells expressing each BioID-tagged protein by 1 μg/ml doxycycline for 1 d were lysed and purified by Streptavidin sepharose. Each protein was detected by its respective antibody. (TIF)
Takao Kitagawa (618877), Koji Nakagawa (424076), Byron Baron (637446), Norie Araki (111307), Durga Paudel (12635367), Yasuhiro Kuramitsu (618885), Daiki Kobayashi (414250), Hajime Okita (240986), Tatsuo Miyamoto (12225655), Yoichi Asaoka (564124), Makoto Furutani-Seiki (24192), Masayuki Tokunaga (13905666), Tohru Ohta (12917207), Rie Takai (13905663), Masanobu Kobayashi (424082)   +14 more
core   +1 more source

Various Strategies for the Immobilization of a Phospholipase C from Bacillus cereus for the Modulation of Its Biochemical Properties

Molecules
In this study, the effect of various immobilization methods on the biochemical properties of phospholipase C (PLC) from Bacillus cereus obtained from the oily soil located in Sfax, Tunisia, was described. Different supports were checked: octyl sepharose,
Ines Abdelkader, Jose M. Guisán, Adel Sayari, Gloria Fernández-Lorente   +3 more
doaj   +1 more source

Active and passive immunizations with HtsA, a streptococcal heme transporter protein, protect mice from subcutaneous group A Streptococcus infection

Journal of Microbiology, Immunology and Infection, 2020
Background/Purpose: HtsA (Streptococcus heme transporter A) is the lipoprotein component of the streptococcal heme ABC transporter (HtsABC). The aim of this study is to investigate whether the HtsA protein has immunoprotective effect against group A ...
Yingli Song, XiaoLan Zhang, Minghui Cai, Chunmei Lv, Yuan Zhao, Deqin Wei, Hui Zhu   +6 more
doaj   +1 more source

Lipoprotein lipase-like activity in the liver of mice with Sarcoma 180.

Journal of Lipid Research, 1984
The triglyceride lipase (TGL) activity of liver homogenates of mice with Sarcoma 180 was measured. The liver homogenate of normal or tumor-bearing mice was treated with 0.25% Triton X-100 and centrifuged at 100,000 g for 60 min, and the supernatant was ...
H Masuno, T Tsujita, H Nakanishi, A Yoshida, R Fukunishi, H Okuda   +5 more
doaj   +1 more source

Purification and Characterization of Porcine Angiotensin Converting Enzyme 2

Shipin gongye ke-ji, 2022
Angiotensin converting enzyme 2 (ACE2) is one of the key enzymes involved in blood pressure regulation. Conventional purification method for ACE2 was complicated and with low efficacy.
Weiwei LIU, Lingjing ZHANG, Mengya JI, Ling WENG, Zhigang ZHANG, Guangming LIU, Minjie CAO   +6 more
doaj   +1 more source

Isolation and Purification of Lipases from Geotrichum Candidum Grown on a Sunflower Oil Waste as a Carbon Source

Chemical Engineering Transactions, 2014
An isolation of extracellular lipases from the endemic fungal strain Geotrichum candidum-M2 was performed by vacuum evaporation, precipitation with cold acetone and vacuum drying.
I. Mladenoska
doaj   +1 more source

Preparation of CCG-1423 Sepharose.

, 2014
(A) Synthesis of the photoaffinity linker, 2-(2-{2-[4-(3-trifluoromethyl-3H-diazirin-3-yl)benzamido]ethoxy}ethoxy)ethylammonium trifluoroacetate. The photoaffinity linker was synthesized by the indicated three-step reactions.
Tsuyoshi Morita (524210), Yoshiaki Nakagawa (524208), Saki Minami (524209), Ken’ichiro Hayashi (524206), Bunta Watanabe (524207)   +4 more
core   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Affinity chromatography of milk pIgGs on anti-kappa-L-Sepharose.

, 2013
The chromatography was performed under the conditions of over-saturation of the affinity capacity of the sorbent (A) and re-chromatography of the preparation eluted by acidic buffer (peak 2) on anti-lambda-L-Sepharose (B) under the conditions of the ...
Valentina N. Buneva (300752), Sergey E. Sedykh (300751), Georgy A. Nevinsky (24945)   +2 more
core   +1 more source