Results 21 to 30 of about 169,356 (299)

Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B

open access: yesResults in Chemistry, 2023
Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro ...
Kun-Hwa Lee   +2 more
doaj   +1 more source

The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]

open access: yes, 1993
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Prescott, Mark   +3 more
core   +1 more source

ISOLATION OF IMMUNOGLOBULIN G FROM CANINE SERUM USING PROTEIN A AFFINITY CHROMATOSRAPHY [PDF]

open access: yesVeterinary Medical Journal - Giza, 1995
Affinity chromatography utilizing protein A apparently covalently bound to sepharose beads was applied to isoalte pure immunoglobulin G from canine serum.
H ELSAID
doaj   +1 more source

Active Subunits of Transaldolase Bound to Sepharose [PDF]

open access: yesEuropean Journal of Biochemistry, 1973
Individual subunits of yeast transaldolase were studied by the method of matrix‐bound derivatives. Native dimeric transaldolase was bound to Sepharose activated with low amounts of cyanogen bromide to give matrix‐bound transaldolase in which the majority of molecules were bound covalently via only one subunit.
W W, Chan, H, Schutt, K, Brand
openaire   +2 more sources

Non-Cryoprecipitation Separation of Coagulation FVIII and Prothrombin Complex Proteins by Pseudoaffinity Calcium Elution Chromatography Using Anion Exchange Resin

open access: yesPharmaceuticals, 2022
Hemophilia A is treated with human plasma coagulation factor VIII (FVIII) replacement therapy and Hemophilia B with coagulation factor IX, which is purified from prothrombin complex concentrate (PCC).
Gabriel Pinna Feliciano   +6 more
doaj   +1 more source

Affinity purification of alkylglycerol monooxygenase from rat liver microsomes by chimyl alcohol-Sepharose 4B column chromatography.

open access: yesJournal of Lipid Research, 1985
Alkylglycerol monooxygenase of rat liver microsomes was purified approximately to 97-fold with a 30% yield by procedures including affinity chromatography on chimyl alcohol-Sepharose 4B.
T Ishibashi, Y Imai
doaj   +1 more source

Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography [PDF]

open access: yes, 2013
In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein.
Rabiatul Adawiyah, Sharom
core   +2 more sources

OPTIMIZATION AND EFFICIENCY IMPROVEMENT OF IN‐HOUSE IMMUNOAFFINITY COLUMN KU-AF02 FOR AFLATOXIN DETECTION

open access: yesBiotropia: The Southeast Asian Journal of Tropical Biology, 2017
In-house immunoaffinity column for aflatoxins (AFs) detection is firstly developed by Kasetsart University since 2011 i.e. the first IAC prototype, KU-AF01.  Detection of aflatoxin B1 (AFB1) up to 200 ng is considered significantly efficient, but the ...
Phakpoom Kooprasertying   +4 more
doaj   +1 more source

Insulin-Sepharose and the Dynamics of Insulin Action [PDF]

open access: yesProceedings of the National Academy of Sciences, 1971
In terms of the rate of accumulation of α-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro . The response to insulin-sepharose is prevented if the
Oka, T, Topper, Y J
openaire   +2 more sources

Sialylation of anti-histone antibodies in blood serum of systemic lupus erythematosus patients

open access: yesБіологічні студії, 2012
Anti-histone autoantibodies belong to main marker antibodies used in the diagnostics of systemic lupus erythematosus (SLE) patients. During autoimmune diseases, especially in SLE, prevalent process of inflammation is caused by high titers of auto ...
I. B. Mahorivska   +5 more
doaj   +1 more source

Home - About - Disclaimer - Privacy