Results 21 to 30 of about 169,356 (299)
Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B
Pyridoxal 5′-phosphate (PLP) is a ubiquitous and versatile cofactor utilized by numerous enzymes involved in amino acid biosynthetic pathways. Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro ...
Kun-Hwa Lee +2 more
doaj +1 more source
The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Prescott, Mark +3 more
core +1 more source
ISOLATION OF IMMUNOGLOBULIN G FROM CANINE SERUM USING PROTEIN A AFFINITY CHROMATOSRAPHY [PDF]
Affinity chromatography utilizing protein A apparently covalently bound to sepharose beads was applied to isoalte pure immunoglobulin G from canine serum.
H ELSAID
doaj +1 more source
Active Subunits of Transaldolase Bound to Sepharose [PDF]
Individual subunits of yeast transaldolase were studied by the method of matrix‐bound derivatives. Native dimeric transaldolase was bound to Sepharose activated with low amounts of cyanogen bromide to give matrix‐bound transaldolase in which the majority of molecules were bound covalently via only one subunit.
W W, Chan, H, Schutt, K, Brand
openaire +2 more sources
Hemophilia A is treated with human plasma coagulation factor VIII (FVIII) replacement therapy and Hemophilia B with coagulation factor IX, which is purified from prothrombin complex concentrate (PCC).
Gabriel Pinna Feliciano +6 more
doaj +1 more source
Alkylglycerol monooxygenase of rat liver microsomes was purified approximately to 97-fold with a 30% yield by procedures including affinity chromatography on chimyl alcohol-Sepharose 4B.
T Ishibashi, Y Imai
doaj +1 more source
Whey protein fractionation using Q-Sepharose anion exchange chromatography and SP-Sepharose cation exchange chromatography [PDF]
In dairy industry, whey protein fractionation is an important process that requires an effective method to separate valuable proteins in bovine whey protein.
Rabiatul Adawiyah, Sharom
core +2 more sources
In-house immunoaffinity column for aflatoxins (AFs) detection is firstly developed by Kasetsart University since 2011 i.e. the first IAC prototype, KU-AF01. Detection of aflatoxin B1 (AFB1) up to 200 ng is considered significantly efficient, but the ...
Phakpoom Kooprasertying +4 more
doaj +1 more source
Insulin-Sepharose and the Dynamics of Insulin Action [PDF]
In terms of the rate of accumulation of α-aminoisobutyric acid, mammary epithelial cells from mature virgin mice respond rapidly to insulin covalently bound to sepharose particles, but do not respond initially to soluble insulin in vitro . The response to insulin-sepharose is prevented if the
Oka, T, Topper, Y J
openaire +2 more sources
Sialylation of anti-histone antibodies in blood serum of systemic lupus erythematosus patients
Anti-histone autoantibodies belong to main marker antibodies used in the diagnostics of systemic lupus erythematosus (SLE) patients. During autoimmune diseases, especially in SLE, prevalent process of inflammation is caused by high titers of auto ...
I. B. Mahorivska +5 more
doaj +1 more source

