Results 11 to 20 of about 169,356 (299)
Chromatography of Hedysarum extracts on modified Sepharose CL sorbents
Фундаментальная и клиническая медицина, 2019 Aim. To study the chromatographic efficiency of modified Sepharose CL-4B sorbents for separation, purification, and preparation of major components from Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb.
Yu. S. Fedorova +4 more
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Chemically-Modified Sepharose 6B Beads for Collection of Circulating Tumor Cells
Biomolecules, 2023 The isolation and quantitative characterization of circulating tumor cells (CTCs) are of great importance in cancer diagnosis and prognosis. However, isolating and detecting CTCs in whole blood presents a significant challenge due to the low numbers of ...
Zhonghua Liu, Bohao Zhou
exaly +3 more sources
, 2007 Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
Moorhead, Greg B.G. +2 more
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Prothrombin-Sepharose-Purified Factor V and Its Role in Prothrombin Conversion
ASEAN Journal on Science and Technology for Development, 2017 Bovine Factor V isolated by the method of Esnouf and Jobin (1967) has been further purified by affiniy chromotgraphy through prothrombin-sepharose. Factor V bound quantitative to the prothrombin-sepharose column.
T.C ONG
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MICROFLUOROMETRIC IMMUNOASSAYS WITH ANTIGENS BOUND TO SEPHAROSE BEADS
Annals of the New York Academy of Sciences, 1975 Sepharose beads, to which protein had been coupled, were used in the development of two types of microfluorometric immunoassays. The first is an inhibition assay: a fixed amount of antiserum was absorbed with different amounts of antigen. This was accomplished in both direct and indirect incubation schemes.
Haaijman, J.J. +2 more
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Molecules, 2021 In this work, the effect of different immobilization procedures on the properties of a lipase obtained from the extremophilic microorganism Serratia sp.
Mónica Ruiz +7 more
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ISOLATION AND PURIFICATION OF A KRINGLE 5 FROM HUMAN PLASMINOGEN USING AH-SEPHAROSE [PDF]
Biotechnologia Acta, 2014 Our aim was to develop a method for isolation of human plasminogen kringle 5 possessing functional activity. The proposed method includes the following steps: hydrolysis of plasminogen with elastase, separation of mini-plasminogen from kringle fragments ...
Kapustianenko L. G. +3 more
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Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose [PDF]
Applied and Environmental Microbiology, 2002 ABSTRACT Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the ...
Patricia A, Shields, Samuel R, Farrah
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RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling. [PDF]
PLoS ONE, 2014 Epithelial-msenchymal transition (EMT) is closely associated with cancer and tissue fibrosis. The nuclear accumulation of myocardin-related transcription factor A (MRTF-A/MAL/MKL1) plays a vital role in EMT.
Ken'ichiro Hayashi +4 more
doaj +1 more source
Purification and characterization of highly stable aldo-keto reductase from camel (Camelus dromedarius) liver [PDF]
Archives of Biological Sciences, 2013 Aldo-keto reductase (AKR) was purified to homogeneity from camel liver by ion exchange on Q Sepharose, affinity chromatography on Blue-Sepharose and 2,5-ADP-Sepharose 4B.
Al-Harbi Ibrahim A. +2 more
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