Results 31 to 40 of about 169,356 (299)
Targeting WDR12 Unleashes T-Cell-Mediated Antitumor Activity in Melanoma by Destabilizing CD276. [PDF]
WDR12 cooperates with the chaperonin subunit CCT7 to maintain CD276 stability on tumor cells, suppressing T‐cell activity and promoting immune escape. SU14813, a small‐molecule WDR12 inhibitor, reduces CD276 stability and relieves CD276‐mediated T‐cell suppression.
Pan J +10 more
europepmc +2 more sources
Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases
A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML ...
Cristina Garcia-Galan +5 more
doaj +1 more source
Protocol for preparing Thiopropyl Sepharose resin used for capturing S-nitrosylated proteins
Summary: S-nitrosothiol (SNO)-Resin Assisted Capture (SNO-RAC) relies on a Thiopropyl Sepharose resin to identify S-nitrosylated proteins (SNO-proteins) and sites of S-nitrosylation.
Puneet Seth +3 more
doaj +1 more source
A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL).
J Boberg +4 more
doaj +1 more source
Extracellular vesicles (EVs) are a versatile group of cell-secreted membranous nanoparticles present in body fluids. They have an exceptional diagnostic potential due to their molecular content matching the originating cells and accessibility from body ...
Vedrana Krušić Alić +11 more
doaj +1 more source
The properties of subunits of avidin coupled to Sepharose [PDF]
Avidin that had been coupled to Sepharose 4B activated with CNBr retained over 90% of its biotin-binding capacity. When low concentrations of CNBr were used about 75% of the protein could be removed from the Sepharose by washing with guanidinium chloride (6 m).
N M, Green, E J, Toms
openaire +2 more sources
‘Amytrapper’, a Novel Immobilized Sepharose API Matrix, Removes Amyloid-β from Circulation
Alzheimer’s disease (AD) is the most common cause of dementia among elderly patients afflicted by neurodegenerative diseases, caused by the accumulation of amyloid-β (Aβ).
Omkar Gandbhir, Pazhani Sundaram
doaj +1 more source
Preparation of antibodies type IgY against Salmonella typhi lipopolysaccharide in chicken eggs
ipopolysaccharide was extracted from local isolate Sallmonella typhi (previously isolated and characterized) by hot EDTA method, and the extract was partially purified by gel filtration chromatography on sepharose Cl-6B gel.
Mouruj A. Alaubydi +2 more
doaj +1 more source
Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. [PDF]
As the proteomics field continues to expand, scientists are looking to integrate cross-disciplinary tools for studying protein structure, function, and interactions. Protein purification remains a key tool for many characterization studies.
Julia G Fraseur, Tamara L Kinzer-Ursem
doaj +1 more source
The Purification of Soybean 11S Globulin with ConA-Sepharose 4B and Sepharose 6B
(1974). The Purification of Soybean 11S Globulin with ConA-Sepharose 4B and Sepharose 6B. Agricultural and Biological Chemistry: Vol. 38, No. 5, pp. 1083-1085.
KITAMURA, Keisuke +2 more
openaire +1 more source

