Results 51 to 60 of about 169,356 (299)

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Purification of type 1 protein (serine/threonine) phosphatases by microcystin-Sepharose affinity chromatography [PDF]

, 1994
A microcystin (MC)-Sepharose column was prepared by addition of 2-aminoethanethiol to the a,ß-unsaturated carbonyl of the N-methyldehydroalanine residue of MC-LR, followed by reaction of the introduced amino group with N-hydroxysuccinimide-activated CH ...
Robert W. MacKintosh, Carol MacKintosh, MacKintosh, Carol, MacKintosh, Carol; id_orcid, Greg Moorhead, Mackintosh, C, Morrice, N, Moorhead, Greg, Morrice, Nick, Moorhead, G, Nick Morrice, Gallagher, Timothy, Gallagher, TC, Mackintosh, RW, Timothy Gallagher, MacKintosh, Robert W.   +15 more
core   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Lab‐in‐a‐Tube System Integrating Hierarchically Wrinkled Electrodes and DNAzyme‐Functionalized Beads for Diagnosis of Clostridioides difficile Infection

Advanced Materials, EarlyView.
The point‐of‐care diagnosis of Clostridioides difficile infection remains challenging due to reliance on slow culture and amplification‐based methods. A lab‐in‐a‐tube electrochemical biosensor integrating DNAzymes, antifouling magnetic beads, and a hierarchically structured flow cell enables amplification‐ and culture‐free detection directly in stool ...
Survanshu Saxena, Soyeon Lee, Youngeun Choi, Jonathan L'Heureux‐Hache, Jiaying Liu, Sadman Sakib, Sara M. Imani, Marek Smieja, Yingfu Li, Todd Hoare, Leyla Soleymani   +10 more
wiley   +1 more source

Evaluation by ELISA of Anisakis simplex Larval Antigen Purified by Affinity Chromatography

Memorias do Instituto Oswaldo Cruz, 2002
In order to improve the specificity and sensitivity of the techniques for the human anisakidosis diagnosis, a method of affinity chromatography for the purification of species-specific antigens from Anisakis simplex third-stage larvae (L3) has been ...
M Rodero, A Jiménez, C Cuéllar
doaj   +1 more source

TOLLIP Inhibits Psoriasis Progression via Suppressing PKM2‐Mediated Glycolysis in Keratinocytes

Advanced Science, EarlyView.
In this study, we identify TOLLIP as a critical regulator of psoriasis pathogenesis through its modulation of glycolytic metabolism. Our findings establish the TOLLIP‐PKM2‐glycolysis axis as a key mechanism linking metabolic reprogramming to psoriasis pathogenesis, and propose TOLLIP as a promising therapeutic target.
Xiuhuan Jiang, Guoying Miao, Na Shen, Qingxia Han, Xiaoru Han, Zichang Qiao, Kexin Feng, Zhuo Tan, Yaguang Wang, Xiuhua Liu, Chen Wu, Zhenzhen Yan   +11 more
wiley   +1 more source

Study on Separation of Phycoerythrin by Q-Sepharose Fast Flow

, 2012
This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verify its feasibility.[Method] Elution gradient,loading volume and flow rate were optimized to determine the optimal separation ...
Gao M(高敏), Wang CQ(王翠芹), Zhang Y(张轶), Gu M(顾铭), Wang ZH(王长海), Zhang B(张彬)   +5 more
core   +1 more source

Selective elution of rodent glutathione S-transferases and glyoxalase I from the S-hexyglutathione-Sepharose affinity matrix [PDF]

, 1988
1. The major hepatic glutathione S-transferases (GSTs) from gerbil, guinea-pig, hamster, mouse and rat comprise Ya- (Mr 25,500-25,800), Yb- (Mr 26,100-26,400), Yc- (Mr 27,000-27,500) and Yf- (Mr 24,800) type subunits. 2.
Hayes, J D
core   +2 more sources

Adiponectin does not bind to gelatin: a new and easy way to purify high-molecular-weight adiponectin from human plasma

Journal of Lipid Research, 2010
Human plasma contains three forms of adiponectin, a trimer, a hexamer, and a high-molecular-weight (HMW) multimer. We previously reported HMW adiponectin was a gelatin-binding protein of 28 kDa (GBP28), it having been purified due to its affinity to ...
Yasuko Nakano, Ayako Shoji, Atsushi Arakawa, Yumiko Iizuka, Yuriko Kikuchi, Maya Kobayashi, Takashi Tobe   +6 more
doaj   +1 more source