Results 51 to 60 of about 26,765,029 (301)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

DNA sequence analysis landscape: a comprehensive review of DNA sequence analysis task types, databases, datasets, word embedding methods, and language models

Frontiers in Medicine
Deoxyribonucleic acid (DNA) serves as fundamental genetic blueprint that governs development, functioning, growth, and reproduction of all living organisms. DNA can be altered through germline and somatic mutations. Germline mutations underlie hereditary
Muhammad Nabeel Asim, Muhammad Nabeel Asim, Muhammad Ali Ibrahim, Muhammad Ali Ibrahim, Arooj Zaib, Andreas Dengel, Andreas Dengel, Andreas Dengel   +7 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Descriptors for DNA Sequences Based on Joint Diagonalization of Their Feature Matrices from Dinucleotide Physicochemical Properties

Tsinghua Science and Technology, 2013
Numerical characterizations of DNA sequence can facilitate analysis of similar sequences. To visualize and compare different DNA sequences in less space, a novel descriptors extraction approach was proposed for numerical characterizations and similarity ...
Hongjie Yu, Deshuang Huang
doaj   +1 more source

A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity. [PDF]

PLoS ONE, 2013
Rad54 is a dsDNA-dependent ATPase that translocates on duplex DNA. Its ATPase function is essential for homologous recombination, a pathway critical for meiotic chromosome segregation, repair of complex DNA damage, and recovery of stalled or broken ...
Xiao-Ping Zhang, Ryan Janke, James Kingsley, Jerry Luo, Clare Fasching, Kirk T Ehmsen, Wolf-Dietrich Heyer   +6 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Phylogenetic analysis of Cryptosporidium isolates from captive reptiles using 18S rDNA sequence data and random amplified polymorphic DNA analys [PDF]

, 1999
Sequence alignment of a polymerase chain reaction-amplified 713-base pair region of the Cryptosporidium 18S rDNA gene was carried out on 15 captive reptile isolates from different geographic locations and compared to both Cryptosporidium parvum and ...
Deplazes, P., Thompson, R.C.A., Fayer, R., Graczyk, T.K., Morgan, U.M., Xiao, L., Lal, A.A.   +6 more
core  

THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI)

BioLink, 2020
Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims
Dewi Indriyani Roslim, Hestia Hairima, Herman Herman, Wahyu Lestari   +3 more
doaj   +1 more source

A sequence-based 163plex microhaplotype assay for forensic DNA analysis

Frontiers in Genetics, 2022
Novel genetic marker microhaplotype has led to an upsurge in forensic genetic research. This study established a 163 microhaplotype (MH) multiplex assay based on next-generation sequencing (NGS) and evaluated the assay’s performance and applicability ...
Ruiyang Tao, Qi Yang, Ruocheng Xia, Xiaochun Zhang, Anqi Chen, Chengtao Li, Suhua Zhang   +6 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source