Results 71 to 80 of about 1,609,143 (313)
A subcloning strategy for DNA sequence analysis
Nucleic Acids Research, 1980 We describe here a new strategy of fragment preparation for sequencing procedures using endlabelled DNA fragments as substrates (2,3) which is directly applicable to DNA fragments cloned into the Pst I site of pBR322, or in modified form, to inserts into the BamH I or Sal I site of the same plasmid.
A M, Frischauf, H, Garoff, H, Lehrach
openaire +3 more sources
, 2009 The analysis of targeted genetic loci from ancient, forensic and clinical samples is usually built upon polymerase chain reaction (PCR)-generated sequence data.
Endicott, Phillip +8 more
core +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Some mathematical problems in genetics
Lietuvos Matematikos Rinkinys, 2007 After an introduction to genetic basics three problems are briefly discussed: microarray data analysis, a definition of noninformative DNA sequence, and genetic sequence alignment. More atention is paid to DNA sequence visualization and regularization of
Marijus Radavičius +2 more
doaj +1 more source
A computer package for DNA sequence analysis
Nucleic Acids Research, 1984 We have developed a comprehensive software-hardware package for a CP/M based or other microcomputer which can perform four major functions in a molecular biology laboratory: (1) the acquisition and analysis of DNA sequence data, (2) word processing in the preparation of scientific manuscripts and figures, (3) general lab computation and data reduction,
Frederick R. Blattner, J. L. Schroeder
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An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Molecular characterization of Colletotrichum species causing Begonia anthracnose in Sri Lanka
Ceylon Journal of Science, 2020 Anthracnose disease is known to affect many tropical and subtropical fruits, vegetables and also certain cut-flowers and foliage plants. The disease was known to be caused by Colletotrichum gloeosporioides or C.
P. Wickramasinghe +2 more
doaj +1 more source
From DNA sequence to application: possibilities and complications [PDF]
, 1999 The development of sophisticated genetic tools during the past 15 years have facilitated a tremendous increase of fundamental and application-oriented knowledge of lactic acid bacteria (LAB) and their bacteriophages.
Venema, Gerard, +8 more
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FEBS Letters, EarlyView.Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil +4 more
wiley +1 more source