Results 71 to 80 of about 1,975,851 (270)

Southern-by-Sequencing: A Robust Screening Approach for Molecular Characterization of Genetically Modified Crops

The Plant Genome, 2015
Molecular characterization of events is an integral part of the advancement process during genetically modified (GM) crop product development. Assessment of these events is traditionally accomplished by polymerase chain reaction (PCR) and Southern blot ...
Gina M. Zastrow-Hayes, Haining Lin, Amy L. Sigmund, Jenna L. Hoffman, Clara M. Alarcon, Kevin R. Hayes, Todd A. Richmond, Jeffery A. Jeddeloh, Gregory D. May, Mary K. Beatty   +9 more
doaj   +1 more source

Disease Severity and Molecular Identification of Banana bunchy top virus, Infecting Local Banana in Bali Island

Jurnal Perlindungan Tanaman Indonesia, 2020
Bunchy top symptoms on banana has been reported in Bali Island since early 2011. Symptoms variation were observed in the field similar to infection of Banana bunchy top virus (BBTV).
Gusti Ngurah Alit Susanta Wirya, I Putu Sudiarta, Dewa Gede Wiryangga Selangga   +2 more
doaj   +1 more source

Subpar reporting of pre‐analytical variables in RNA‐focused blood plasma studies

Molecular Oncology, EarlyView.
Pre‐analytical variables strongly influence the analysis of extracellular RNA (cell‐free RNA; exRNA) derived from blood plasma. Their reporting is essential to allow interpretation and replication of results. By evaluating 200 exRNA studies, we pinpoint a lack of reporting pre‐analytical variables associated with blood collection, plasma preparation ...
Céleste Van Der Schueren, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Laudonia Lidia Dipalo, Hetty Hilde Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, Anneleen Decock   +16 more
wiley   +1 more source

Molecular characterization of Colletotrichum species causing Begonia anthracnose in Sri Lanka

Ceylon Journal of Science, 2020
Anthracnose disease is known to affect many tropical and subtropical fruits, vegetables and also certain cut-flowers and foliage plants. The disease was known to be caused by Colletotrichum gloeosporioides or C.
P. Wickramasinghe, N. Adikaram, D. Yakandawala   +2 more
doaj   +1 more source

DeepSNVMiner: a sequence analysis tool to detect emergent, rare mutations in subsets of cell populations [PDF]

PeerJ, 2016
Background. Massively parallel sequencing technology is being used to sequence highly diverse populations of DNA such as that derived from heterogeneous cell mixtures containing both wild-type and disease-related states.
T. Daniel Andrews, Yogesh Jeelall, Dipti Talaulikar, Christopher C. Goodnow, Matthew A. Field   +4 more
doaj   +2 more sources

A computer package for DNA sequence analysis

Nucleic Acids Research, 1984
We have developed a comprehensive software-hardware package for a CP/M based or other microcomputer which can perform four major functions in a molecular biology laboratory: (1) the acquisition and analysis of DNA sequence data, (2) word processing in the preparation of scientific manuscripts and figures, (3) general lab computation and data reduction,
John L. Schroeder, Frederick R. Blattner
openaire   +4 more sources

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source

Clinical applications of next‐generation sequencing‐based ctDNA analyses in breast cancer: defining treatment targets and dynamic changes during disease progression

Molecular Oncology, EarlyView.
Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker, Samantha Hasenleithner, Rupert Bartsch, Simon P. Gampenrieder, Daniel Egle, Christian F. Singer, Gabriel Rinnerthaler, Michael Hubalek, Katja Schmitz, Zsuzsanna Bago‐Horvath, Andreas Petzer, Sonja Heibl, Ellen Heitzer, Marija Balic, Michael Gnant   +14 more
wiley   +1 more source

Efficiency of peptide nucleic acid-directed PCR clamping and its application in the investigation of natural diets of the Japanese eel leptocephali. [PDF]

PLoS ONE, 2011
Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis.
Takeshi Terahara, Seinen Chow, Hiroaki Kurogi, Sun-Hee Lee, Katsumi Tsukamoto, Noritaka Mochioka, Hideki Tanaka, Haruko Takeyama   +7 more
doaj   +1 more source

Bubble statistics and positioning in superhelically stressed DNA [PDF]

, 2011
We present a general framework to study the thermodynamic denaturation of double-stranded DNA under superhelical stress. We report calculations of position- and size-dependent opening probabilities for bubbles along the sequence. Our results are obtained from transfer-matrix solutions of the Zimm-Bragg model for unconstrained DNA and of a self ...
arxiv   +1 more source