Results 101 to 110 of about 1,742,697 (207)

Assaying the Effects of Splice Site Variants by Exon Trapping in a Mammalian Cell Line

Bio-Protocol, 2017
There are several in silico programs that endeavor to predict the functional impact of an individual’s sequence variation at splice donor/acceptor sites, but experimental confirmation is problematic without a source of RNA from the individual that ...
Stuart Tompson, Terri Young
doaj   +1 more source

High-Resolution RNA Maps Suggest Common Principles of Splicing and Polyadenylation Regulation by TDP-43

Cell Reports, 2017
Summary: Many RNA-binding proteins (RBPs) regulate both alternative exons and poly(A) site selection. To understand their regulatory principles, we developed expressRNA, a web platform encompassing computational tools for integration of iCLIP and RNA ...
Gregor Rot, Zhen Wang, Ina Huppertz, Miha Modic, Tina Lenče, Martina Hallegger, Nejc Haberman, Tomaž Curk, Christian von Mering, Jernej Ule   +9 more
doaj   +1 more source

Sequence analysis of immunoglobulin light chain messenger RNA

Nature, 1974
RNA and DNA sequencing methods are used to define some features, principally at the 3′ terminus, of the structure of immunoglobulin light chain mRNA. The results show that there is one molecule coding for the V and C regions of the light chain and rule out the possibility of two separate molecules.
Milstein, C, Brownlee, G G, Cartwright, E M, Jarvis, J M, Proudfoot, N J   +4 more
openaire   +2 more sources

Bistability of an In Vitro Synthetic Autoregulatory Switch [PDF]

, 2011
The construction of synthetic biochemical circuits is an essential step for developing quantitative understanding of information processing in natural organisms.
Kim, Jongmin, Subsoontorn, Pakpoom, Winfree, Erik   +2 more
core  

A mutate-and-map protocol for inferring base pairs in structured RNA

, 2013
Chemical mapping is a widespread technique for structural analysis of nucleic acids in which a molecule's reactivity to different probes is quantified at single-nucleotide resolution and used to constrain structural modeling.
CS Chow, D Moazed, DH Mathews, EE Regulski, JB Lucks, JG Underwood, JP Staley, KA Wilkinson, M Kertesz, M Mandal, N Kulshina, NB Leontis, P Cordero, P Cordero, P Rocca-Serra, P Tijerina, PJ Lukavsky, R Rydzanicz, RC Spitale, RF Gesteland, S Mitra, S Washietl, S Yoon, SA Mortimer, SE Wells, SM Vasa, SW Brostoff, VD Sood, W Kladwang, W Kladwang, W Kladwang, W Kladwang, Y Rhee   +32 more
core   +1 more source

Incorporating graph representation and mutual attention mechanism for MiRNA-MRNA interaction prediction

Frontiers in Genetics
IntroductionPredicting interactions between microRNAs (miRNAs) and messenger RNAs (mRNAs) is crucial for understanding gene expression regulation mechanisms and their roles in diseases.
Tai-Long Shi, Lei Wang, Lei Wang, Leon Wong, Zhu-Hong You, Chang-Qing Yu, Chen Jiang, Si-Zhe Liang   +7 more
doaj   +1 more source

YModPred: an interpretable prediction method for multi-type RNA modification sites in S. cerevisiae based on deep learning

BMC Biology
Background RNA post-transcriptional modifications involve the addition of chemical groups to RNA molecules or alterations to their local structure. These modifications can change RNA base pairing, affect thermal stability, and influence RNA folding ...
Chunyan Ao, Mengting Niu, Quan Zou, Liang Yu, Yansu Wang   +4 more
doaj   +1 more source

Recommendations for detection, validation, and evaluation of RNA editing events in cardiovascular and neurological/neurodegenerative diseases

Molecular Therapy: Nucleic Acids
RNA editing, a common and potentially highly functional form of RNA modification, encompasses two different RNA modifications, namely adenosine to inosine (A-to-I) and cytidine to uridine (C-to-U) editing. As inosines are interpreted as guanosines by the
Korina Karagianni, Alessia Bibi, Alisia Madé, Shubhra Acharya, Mikko Parkkonen, Teodora Barbalata, Prashant K. Srivastava, David de Gonzalo-Calvo, Constanza Emanueli, Fabio Martelli, Yvan Devaux, Dimitra Dafou, A. Yaël Nossent   +12 more
doaj   +1 more source

PCR mediated analysis of RNA sequences

Nucleic Acids Research, 1993
M, Hetzer, M W, Mueller
openaire   +3 more sources

Optimized library preparation, sequencing, and data analysis protocols for the generation of orbivirus consensus sequences. [PDF]

BMC Genomics
Dunham TJ, Sherman TJ, Reed KJ, Brelsfoard C, Anderson TK, Cohnstaedt LW, Stenglein MD, Mayo CE.   +7 more
europepmc   +1 more source