ScanFold: an approach for genome-wide discovery of local RNA structural elements—applications to Zika virus and HIV [PDF]
PeerJ, 2018 In addition to encoding RNA primary structures, genomes also encode RNA secondary and tertiary structures that play roles in gene regulation and, in the case of RNA viruses, genome replication.
Ryan J. Andrews +2 more
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Primary structure and functional expression of a cyclic nucleotidegated channel from rabbit aorta [PDF]
, 1993 Sequences specific for cyclic nucleotide-gated channels (CNG channels) have been amplified by PCR from cDNA of heart, aorta, sinoatrial node, cerebellum, C-cells and kidney.
Ahmad +27 more
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Global analysis of RNA cleavage by 5′-hydroxyl RNA sequencing [PDF]
Nucleic Acids Research, 2015 RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5'-hydroxyl (5'-OH) and 2',3'-cyclic phosphate termini. To identify 5'-OH RNA fragments produced by these cleavage events, we exploited the unique ligation mechanism of Escherichia coli RtcB RNA ligase to attach an oligonucleotide linker to RNAs with 5'-OH ...
Kerri York +2 more
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Expanding the repertoire of human tandem repeat RNA-binding proteins.
PLoS ONE, 2023 Protein regions consisting of arrays of tandem repeats are known to bind other molecular partners, including nucleic acid molecules. Although the interactions between repeat proteins and DNA are already widely explored, studies characterising tandem ...
Agustín Ormazábal +6 more
doaj +1 more source
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]
, 2020 Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL +3 more
core
Spatial transcriptomics of the nematode Caenorhabditis elegans using RNA tomography
STAR Protocols, 2021 Summary: RNA tomography or tomo-seq combines mRNA sequencing and cryo-sectioning to spatially resolve gene expression. We have adapted this method for the nematode Caenorhabditis elegans to generate anteroposterior gene expression maps at near-cellular ...
Erik S. Schild +5 more
doaj
An Rna Sequencing Analysis of Glaucoma Genesis in Mice
Artificial Intelligence, Soft Computing and Applications, 2022 Glaucoma is the leading cause of irreversible blindness in people over the age of 60, accounting for 6.6 to 8% of all blindness in 2010, but there is still much to be learned about the genetic origins of the eye disease. With the modern development of Next-Generation Sequencing (NGS) technologies, scientists are starting to learn more about the genetic
Sharma, Jai, Bhaskar, Vidhyacharan
openaire +2 more sources
Dataset of the transcribed 45S ribosomal RNA sequence of the tree crop yerba mate [PDF]
, 2017 This contribution contains data related to the research article entitled The 18S-25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.) (Aguilera et al., 2016).
Aguilera, Patricia Mabel +2 more
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Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines [PDF]
, 2017 Neuroblastoma cell lines are an important and cost-effective model used to study oncogenic drivers of the disease. While many of these cell lines have been previously characterized with SNP, methylation, and/or mRNA expression microarrays, there has not ...
Adams, Rebecca N. +9 more
core +8 more sources
The First Complete Genome Sequence of a Novel Tetrastichus brontispae RNA Virus-1 (TbRV-1)
Viruses, 2019 The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins ...
E Meng +8 more
doaj +1 more source