Identification and sequence determination of a novel double-stranded RNA mycovirus from the entopathogenic fungus Beauveria bassina [PDF]
, 2015 The final publication is available at Springer via https://doi.org/10.1007/s00705-014-2332-8.An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five double-stranded (ds) RNA elements ranging from 1.5 to more than 3 kbp. The
Coutts, Robert H.A. +2 more
core +1 more source
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]
, 2020 Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL +3 more
core
HeliyonDeciphering information of RNA sequences reveals their diverse roles in living organisms, including gene regulation and protein synthesis. Aberrations in RNA sequence such as dysregulation and mutations can drive a diverse spectrum of diseases including ...
Muhammad Nabeel Asim +3 more
doaj +1 more source
DNApi: A De Novo Adapter Prediction Algorithm for Small RNA Sequencing Data. [PDF]
PLoS ONE, 2016 With the rapid accumulation of publicly available small RNA sequencing datasets, third-party meta-analysis across many datasets is becoming increasingly powerful.
Junko Tsuji, Zhiping Weng
doaj +1 more source
Transcriptomic and ChIP-sequence interrogation of EGFR signaling in HER2+ breast cancer cells reveals a dynamic chromatin landscape and S100 genes as targets. [PDF]
, 2019 BACKGROUND:The Human Epidermal Growth Factor Receptor (EGFR/HER1) can be activated by several ligands including Transforming Growth Factor alpha (TGF-α) and Epidermal Growth Factor (EGF).
Dutta, Pranabananda +5 more
core +5 more sources
Comparative Analysis of Single-Cell RNA Sequencing Methods [PDF]
Molecular Cell, 2017 Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and medical questions. However, systematic comparisons of the performance of diverse scRNA-seq protocols are lacking. We generated data from 583 mouse embryonic stem cells to evaluate six prominent scRNA-seq methods: CEL-seq2, Drop-seq, MARS-seq, SCRB-seq, Smart-seq ...
Ziegenhain, Christoph +9 more
openaire +3 more sources
陆军军医大学学报Objective To analyze the composition characteristics and biological functions of tumor cell subpopulations in glioblastoma (GBM) through multi-transcriptomics sequencing technology, and explore the hypoxia characteristics and spatial localization ...
CHEN Tao, YANG Fan, WANG Shuai
doaj +1 more source
Probing of RNA structures in a positive sense RNA virus reveals selection pressures for structural elements. [PDF]
, 2017 In single stranded (+)-sense RNA viruses, RNA structural elements (SEs) play essential roles in the infection process from replication to encapsidation.
Aviran, Sharon +5 more
core +2 more sources
Global analysis of RNA cleavage by 5′-hydroxyl RNA sequencing [PDF]
Nucleic Acids Research, 2015 RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5'-hydroxyl (5'-OH) and 2',3'-cyclic phosphate termini. To identify 5'-OH RNA fragments produced by these cleavage events, we exploited the unique ligation mechanism of Escherichia coli RtcB RNA ligase to attach an oligonucleotide linker to RNAs with 5'-OH ...
Peach, Sally E. +2 more
openaire +2 more sources
Protocol for identifying differentially expressed genes using the RumBall RNA-seq analysis platform
STAR ProtocolsSummary: Here, we present a protocol for the identification of differentially expressed genes through RNA sequencing analysis. Starting with FASTQ files from public datasets, this protocol leverages RumBall within a self-contained Docker system.
Luis Augusto Eijy Nagai +2 more
doaj +1 more source