Results 71 to 80 of about 3,346,579 (352)

Specific and non specific hybridization of oligonucleotide probes on microarrays [PDF]

, 2005
Gene expression analysis by means of microarrays is based on the sequence specific binding of mRNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving other sequences than the intended target
Binder, Hans, Preibisch, Stephan
core   +3 more sources

The immunological interface: dendritic cells as key regulators in metabolic dysfunction‐associated steatotic liver disease

FEBS Letters, EarlyView.
Metabolic dysfunction‐associated steatotic liver disease (MASLD) affects nearly one‐third of the global population and poses a significant risk of progression to cirrhosis or liver cancer. Here, we discuss the roles of hepatic dendritic cell subtypes in MASLD, highlighting their distinct contributions to disease initiation and progression, and their ...
Camilla Klaimi, WanTing Kong, Camille Blériot, Joel T. Haas   +3 more
wiley   +1 more source

A Beginner’s Guide to Analysis of RNA Sequencing Data [PDF]

American Journal of Respiratory Cell and Molecular Biology, 2018
Since the first publications coining the term RNA-seq (RNA sequencing) appeared in 2008, the number of publications containing RNA-seq data has grown exponentially, hitting an all-time high of 2,808 publications in 2016 (PubMed). With this wealth of RNA-seq data being generated, it is a challenge to extract maximal meaning from these datasets, and ...
Elizabeth T. Bartom, Deborah R. Winter, Karen M. Ridge, Ankit Bharat, Clarissa M. Koch, Mahzad Akbarpour, Stephen Chiu   +6 more
openaire   +3 more sources

Transcriptome Analysis May Be Beneficial for Identification of Specific Pathways in Host Cell-Leishmania major Interactions(letter to editor)

Medical Laboratory Journal, 2022
Dear Editor, There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells.
Khodaberdi Kalavi, Mohammad Ali Faghihi, Ogholniaz Jorjani, Zolikha Tatari   +3 more
doaj  

Hantavirus in African Wood Mouse, Guinea

Emerging Infectious Diseases, 2006
Hantaviruses are rodentborne, emerging viruses that cause life-threatening human diseases in Eurasia and the Americas. We detected hantavirus genome sequences in an African wood mouse (Hylomyscus simus) captured in Sangassou, Guinea.
Boris Klempa, Elisabeth Fichet-Calvet, Emilie Lecompte, Brita Auste, Vladimir Aniskin, Helga Meisel, Christiane Denys, Lamine Koivogui, Jan ter Meulen, Detlev H. Krüger   +9 more
doaj   +1 more source

Sequence motifs that distinguish ATP(CTP) : tRNA nucleotidyl transferases from eubacterial poly(A) polymerases [PDF]

, 2004
ATP(CTP):tRNA nucleotidyl transferases, tRNA maturing enzymes found in all organisms, and eubacterial poly(A) polymerases, enzymes involved in mRNA degradation, are so similar that until now their biochemical functions could not be distinguished by their
Keller, W., Martin, G.
core   +2 more sources

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

FEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi   +6 more
wiley   +1 more source

Analysis workflow of publicly available RNA-sequencing datasets

STAR Protocols, 2021
Differential gene expression analysis is widely used to study changes in gene expression profiles between two or more groups of samples (e.g., physiological versus pathological conditions, pre-treatment versus post-treatment, and infected versus non-infected tissues). This protocol aims to identify gene expression changes in a pre-selected set of genes
Pablo Sanchis, Rosario Lavignolle, Mercedes Abbate, Sofía Lage-Vickers, Elba Vazquez, Javier Cotignola, Juan Bizzotto, Geraldine Gueron   +7 more
openaire   +5 more sources

Modeling and analysis of RNA-seq data: a review from a statistical perspective [PDF]

Quantitative Biology 6 (2018) 195-209, 2018
Background: Since the invention of next-generation RNA sequencing (RNA-seq) technologies, they have become a powerful tool to study the presence and quantity of RNA molecules in biological samples and have revolutionized transcriptomic studies. The analysis of RNA-seq data at four different levels (samples, genes, transcripts, and exons) involve ...
arxiv   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source