Results 11 to 20 of about 104,871 (266)

Modeling of tertiary and quaternary protein structures by homology [PDF]

open access: yes, 2012
The structure of a protein is crucial to understand its function. Despite this importance, experimentally solved structures are only available for a small portion of the currently known protein sequences.
Kiefer, Florian
core   +1 more source

Of bits and bugs [PDF]

open access: yes, 2010
Pur-α is a nucleic acid-binding protein involved in cell cycle control, transcription, and neuronal function. Initially no prediction of the three-dimensional structure of Pur-α was possible.
Graebsch, Almut   +4 more
core   +1 more source

Analysis and forecasting of global real time RT-PCR primers and probes for SARS-CoV-2

open access: yesScientific Reports, 2021
Rapid tests for active SARS-CoV-2 infections rely on reverse transcription polymerase chain reaction (RT-PCR). RT-PCR uses reverse transcription of RNA into complementary DNA (cDNA) and amplification of specific DNA (primer and probe) targets using ...
Gowri Nayar   +6 more
doaj   +1 more source

Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases

open access: yesBiomolecules, 2022
In precise genome editing, site-specific DNA double-strand breaks (DSBs) induced by the CRISPR/Cas9 system are repaired via homology-directed repair (HDR) using exogenous donor DNA templates.
Wataru Aiba   +3 more
doaj   +1 more source

Wound healing potential: evaluation of molecular profiling and amplification of Lucilia sericata angiopoietin-1 mRNA mid-part

open access: yesBMC Research Notes, 2020
Objective High prevalence of chronic ulcers and the burden of disease necessitate the increasingly significant production of new recombinant proteins in the world.
Hamzeh Alipour   +3 more
doaj   +1 more source

A novel cold-regulated cold shock domain containing protein from scallop Chlamys farreri with nucleic acid-binding activity. [PDF]

open access: yesPLoS ONE, 2012
BACKGROUND: The cold shock domain (CSD) containing proteins (CSDPs) are one group of the evolutionarily conserved nucleic acid-binding proteins widely distributed in bacteria, plants, animals, and involved in various cellular processes, including ...
Chuanyan Yang   +7 more
doaj   +1 more source

Molecular detection of parvovirus in Manchurian chipmunks (Tamias sibiricus asiaticus) captured in Korea

open access: yesIntervirology, 2021
Cross-species transmission of viral diseases alarms our global community for its potential of novel pandemic events. Of various viral pathogens noted recently, parvoviruses have posed public health threats not only to humans but to wild animals.
Jin Il Kim   +4 more
doaj   +1 more source

Identification and Characterization of a Garlic Virus E Genome in Garlic (Allium sativum L.) Using High-Throughput Sequencing from India

open access: yesPlants, 2022
Garlic (Allium sativum L.) plants exhibiting mosaics, deformation, and yellow stripes symptoms were identified in Meerut City, Uttar Pradesh, India. To investigate the viruses in the garlic samples, the method of high-throughput sequencing (HTS) was used.
Malyaj R. Prajapati   +7 more
doaj   +1 more source

Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR [PDF]

open access: yesPakistan Veterinary Journal, 2012
To investigate, whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province, China. Total DNA from these mosquitoes was extracted and
Xiaohong Yang, Lidan Hou, Jing Ye, Qigai He and Shengbo Cao*
doaj  

Investigation of differences between wheat and barley forms of Wheat dwarf virus and their distribution in host plants

open access: yesPlant Protection Science, 2002
Wheat dwarf virus, a monogemini virus, infects several cereal species. Until now complete sequence data have been published only for wheat isolates. We cloned the complete DNA of 21 isolates from wheat, barley and Lolium spec.
J. Schubert, A. HABEKUß, F. Rabenstein
doaj   +1 more source

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