Results 111 to 120 of about 5,999 (124)

Isolation of New Chemical Modulators of the Interaction Between HIV-1 Integrase and the Cellular Restriction Factor GCN2. [PDF]

Viruses
Torres C, Lagadec F, Basyuk E, Recordon-Pinson P, Métifiot M.   +4 more
europepmc   +1 more source

Abstract 2425 Large serine integrases: how do they know which way to go?

Journal of Biological Chemistry
Phoebe Rice, Heewhan Shin, Femi Olorunniji, Phoebe Rice   +3 more
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Serine Integrase-Based Event Recording in E. coli

2022
DNA is a unique molecule that has evolved to serve as the genetic material for life. It seems straightforward to consider this molecule not only as a wonder of the natural world but as a tool for information storage and retrieval. Bacteria have evolved to conserve DNA, but bacteriophages have evolved to specifically integrate their genomes using ...
openaire   +1 more source

Site-specific recombination by φC31 integrase and other large serine recombinases

Biochemical Society Transactions, 2010
Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the λ Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently in vitro in the presence of their cognate (~50 bp) phage ...
Margaret C M, Smith, William R A, Brown, Andrew R, McEwan, Paul A, Rowley   +3 more
openaire   +2 more sources

Serine integrase recombinational engineering (SIRE): A versatile toolbox for genome editing

Biotechnology and Bioengineering, 2018
AbstractChromosomal integration of biosynthetic pathways for the biotechnological production of high‐value chemicals is a necessity to develop industrial strains with a high long‐term stability and a low production variability. However, the introduction of multiple transcription units into the microbial genome remains a difficult task.
Nico Snoeck, Maarten L. De Mol, Dries Van Herpe, Anke Goormans, Isabelle Maryns, Pieter Coussement, Gert Peters, Joeri Beauprez, Sofie L. De Maeseneire, Wim Soetaert   +9 more
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Establishing a Serine Integrase‐Based Genetic Memory System In Vitro

Biotechnology and Bioengineering
ABSTRACTThe increasing demand for advanced biosystems necessitates innovative approaches to store and process genetic information. DNA, as a high‐density storage medium, offers a promising solution for creating genetic memory systems that can provide state‐dependent responses to various stimuli. To date, numerous studies have reported on genetic memory
Luyao Wang, Fang Ba, Yufei Zhang, Wan‐Qiu Liu, Jian Li   +4 more
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Orthogonal Serine Integrases Enable Scalable Gene Storage Cascades in Bacterial Genome

ACS Synthetic Biology
Genome integration enables host organisms to stably carry heterologous DNA messages, introducing new genotypes and phenotypes for expanded applications. While several genome integration approaches have been reported, a scalable tool for DNA message storage within site-specific genome landing pads is still lacking. Here, we introduce an iterative genome
Yufei Zhang, Fang Ba, Shuhui Huang, Wan-Qiu Liu, Jian Li   +4 more
openaire   +2 more sources

A diversity of serine phage integrases mediate site-specific recombination in mammalian cells

Molecular Genetics and Genomics, 2006
This study evaluated the ability of five serine phage integrases, from phages A118, U153, Bxb1, phiFC1, and phiRV1, to mediate recombination in mammalian cells. Two types of recombination were investigated, including the ability of an integrase to mediate recombination between its own phage att sites in the context of a mammalian cell and the ability ...
Annahita, Keravala, Amy C, Groth, Sohail, Jarrahian, Bhaskar, Thyagarajan, Jason J, Hoyt, Patrick J, Kirby, Michele P, Calos   +6 more
openaire   +2 more sources

Rapid Optimization of Engineered Metabolic Pathways with Serine Integrase Recombinational Assembly (SIRA)

2016
Metabolic pathway engineering in microbial hosts for heterologous biosynthesis of commodity compounds and fine chemicals offers a cheaper, greener, and more reliable method of production than does chemical synthesis. However, engineering metabolic pathways within a microbe is a complicated process: levels of gene expression, protein stability, enzyme ...
C A, Merrick, C, Wardrope, J E, Paget, S D, Colloms, S J, Rosser   +4 more
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Reprogrammed Serine Integrases Enable Precise Integration of Synthetic DNA

Summary Despite recent progress in the ability to manipulate the genomes of eukaryotic cells1–3, there is still no effective and practical method to precisely integrate large synthetic DNA constructs into desired chromosomal sites using a programmable integrase. Serine integrases can perform the necessary molecular steps4, but only if their
Friedrich Fauser, Sebastian Arangundy-Franklin, Jessica E Davis, Lifeng Liu, Nicola J Schmidt, Luis Rodriguez, Danny F Xia, Nga Nguyen, Nicholas A Scarlott, Rakshaa Mureli, Irene Tan, Yuanyue Zhou, Lynn N Truong, Sarah J Hinkley, Bhakti N Kadam, Stephen Lam, Bryan Bourgeois, Emily Tait, Mohammad Qasim, Vishvesha Vaidya, Adeline Chen, Andrew Nguyen, Yuri R. Bendaña, David A. Shivak, Patrick Li, Andreas Reik, David E Paschon, Gregory D Davis, Jeffrey C Miller   +28 more
openaire   +1 more source