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Characterization of urinary Escherichia coli isolates in HIV-seropositive women with asymptomatic bacteriuria in Western Ghana. [PDF]

BMC Microbiol
Amegbletor HK, Labi AK, Hukporti N, Osei MM, Cooper P, Owusu F, Amuasi GR, Mensah EK, Egyir B, Opintan JA.   +9 more
europepmc   +1 more source

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Establishing a Serine Integrase‐Based Genetic Memory System In Vitro

Biotechnology and Bioengineering
The increasing demand for advanced biosystems necessitates innovative approaches to store and process genetic information. DNA, as a high‐density storage medium, offers a promising solution for creating genetic memory systems that can provide state ...
Luyao Wang, Fang Ba, Yufei Zhang, Wanqiu Liu, Jian Li   +4 more
semanticscholar   +3 more sources

Serine integrase recombinational engineering (SIRE): A versatile toolbox for genome editing

Biotechnology and Bioengineering, 2018
Chromosomal integration of biosynthetic pathways for the biotechnological production of high‐value chemicals is a necessity to develop industrial strains with a high long‐term stability and a low production variability.
Nico Snoeck, M. D. De Mol, Dries Van Herpe, Anke Goormans, I. Maryns, P. Coussement, Gert Peters, Joeri J. Beauprez, S. D. De Maeseneire, W. Soetaert   +9 more
semanticscholar   +3 more sources

Serine Integrase Structure and Function

The FASEB Journal, 2011
G. V. Van Duyne, K. Rutherford, Kushol Gupta   +2 more
semanticscholar   +2 more sources

Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family. [PDF]

Methods in molecular biology, 2017
Assembling multiple DNA fragments into functional plasmids is an important and often rate-limiting step in engineering new functions in living systems. Bacteriophage integrases are enzymes that carry out efficient recombination reactions between short, defined DNA sequences known as att sites.
F. Olorunniji, Christine A Merrick, S. Rosser, Margaret C. M. Smith, W. Stark, S. Colloms   +5 more
semanticscholar   +3 more sources

In vivo and in vitro characterization of site-specific recombination of a novel serine integrase from the temperate phage EFC-1.

Biochemical and Biophysical Research Communications, 2016
B. Yoon, Inki Kim, Ja-Ae Nam, H. Chang, C. Ha   +4 more
semanticscholar   +3 more sources

Rapid Optimization of Engineered Metabolic Pathways with Serine Integrase Recombinational Assembly (SIRA).

Methods in Enzymology, 2016
Metabolic pathway engineering in microbial hosts for heterologous biosynthesis of commodity compounds and fine chemicals offers a cheaper, greener, and more reliable method of production than does chemical synthesis. However, engineering metabolic pathways within a microbe is a complicated process: levels of gene expression, protein stability, enzyme ...
Christine A Merrick, Caroline Wardrope, J. E. Paget, S. Colloms, Susan J. Rosser   +4 more
semanticscholar   +3 more sources

Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii.

Metabolic Engineering, 2019
The real value of gas-fermenting clostridia, capable of using CO and CO2, resides in their potential of being developed into cell factories to produce various bulk chemicals and fuels.
He Huang, Changsheng Chai, Sheng Yang, Weihong Jiang, Yang Gu   +4 more
semanticscholar   +1 more source

Serine Integrase-Based Event Recording in E. coli

2022
DNA is a unique molecule that has evolved to serve as the genetic material for life. It seems straightforward to consider this molecule not only as a wonder of the natural world but as a tool for information storage and retrieval. Bacteria have evolved to conserve DNA, but bacteriophages have evolved to specifically integrate their genomes using ...
openaire   +1 more source

Site-specific recombination by φC31 integrase and other large serine recombinases

Biochemical Society Transactions, 2010
Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the λ Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently in vitro in the presence of their cognate (~50 bp) phage ...
Margaret C M, Smith, William R A, Brown, Andrew R, McEwan, Paul A, Rowley   +3 more
openaire   +2 more sources