Results 21 to 30 of about 120,836 (163)

Serotypes of bovine rotaviruses distinguished by serum neutralization [PDF]

Infection and Immunity, 1983
Fourteen cytopathic bovine rotaviruses isolated from naturally affected calves were characterized serologically by serum neutralization. Antisera were prepared in guinea pigs against the three isolates and the Lincoln strain of bovine rotavirus. These four rotaviruses were segregated into at least two distinct serotypes (designated types 1 and 2) by ...
Y, Murakami, N, Nishioka, Y, Hashiguchi, C, Kuniyasu   +3 more
openaire   +2 more sources

Hepatitis E virus neutralization by porcine serum antibodies

Journal of Clinical Microbiology, 2023
ABSTRACT The consumption of raw or undercooked meat products poses a serious risk for human hepatitis E virus (HEV) infections. In many high-income countries, domestic pigs and wild boars represent the main animal reservoirs for HEV and are usually identified by reverse transcription-PCR and antibody enzyme-linked immunosorbent assay (ELISA).
Nele Gremmel, Oliver Keuling, Martin Eiden, Martin H. Groschup, Reimar Johne, Paul Becher, Christine Baechlein   +6 more
openaire   +2 more sources

Serotypic evolution of measles virus is constrained by multiple co-dominant B cell epitopes on its surface glycoproteins

Cell Reports Medicine, 2021
Summary: After centuries of pestilence and decades of global vaccination, measles virus (MeV) genotypes capable of evading vaccine-induced immunity have not emerged. Here, by systematically building mutations into the hemagglutinin (H) glycoprotein of an
Miguel Ángel Muñoz-Alía, Rebecca A. Nace, Lianwen Zhang, Stephen J. Russell   +3 more
doaj   +1 more source

Ensaio imunoenzimático comercial no diagnóstico sorológico das infecções por herpesvírus bovino 1 A commercial enzyme immune assay in serodiagnosis of bovine herpesvirus 1 infections

Ciência Rural, 2000
Avaliou-se o desempenho de um ensaio imunoenzimático, obtido de fonte comercial, na identificação de anticorpos contra herpesvírus bovino tipo 1 (BHV-1), induzidos tanto por infecção natural quanto por vacinação, em 1000 amostras de soros sangüíneos de ...
Kerlei Cristina Médici, Amauri Alcindo Alfieri, Alice Fernandes Alfieri   +2 more
doaj   +1 more source

Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA Assay

Vaccines, 2021
This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum.
Ahmed O. Shalash, Armira Azuar, Harrison Y. R. Madge, Naphak Modhiran, Alberto A. Amarilla, Benjamin Liang, Alexander A. Khromykh, Daniel Watterson, Paul R. Young, Istvan Toth, Mariusz Skwarczynski   +10 more
doaj   +1 more source

Multiple Assessment and Serum Neutralization of Arbovirus Mixtures [PDF]

Applied Microbiology, 1970
Mixtures of Venezuelan equine encephalomyelitis, Rift Valley fever, and chikungunya viruses may be assayed by selective immunofluorescence staining of infected cell monolayers. A multiple serum neutralization test is described for quantifying reactions of these viruses with mixtures of serum antibodies.
N, Hahon, C J, Mayhew
openaire   +2 more sources

Saturation time of exposure interval for cross-neutralization response to SARS-CoV-2: Implications for vaccine dose interval

iScience, 2023
Summary: Evaluating the serum cross-neutralization responses after breakthrough infection with various SARS-CoV-2 variants provides valuable insight for developing variant-proof COVID-19 booster vaccines.
Sho Miyamoto, Yudai Kuroda, Takayuki Kanno, Akira Ueno, Nozomi Shiwa-Sudo, Naoko Iwata-Yoshikawa, Yusuke Sakai, Noriyo Nagata, Takeshi Arashiro, Akira Ainai, Saya Moriyama, Noriko Kishida, Shinji Watanabe, Kiyoko Nojima, Yohei Seki, Takuo Mizukami, Hideki Hasegawa, Hideki Ebihara, Shuetsu Fukushi, Yoshimasa Takahashi, Ken Maeda, Tadaki Suzuki   +21 more
doaj   +1 more source

Distinct Features and Functions of Systemic and Mucosal Humoral Immunity Among SARS-CoV-2 Convalescent Individuals

Frontiers in Immunology, 2021
Understanding humoral immune responses to SARS-CoV-2 infection will play a critical role in the development of vaccines and antibody-based interventions.
Savannah E. Butler, Andrew R. Crowley, Harini Natarajan, Shiwei Xu, Joshua A. Weiner, Carly A. Bobak, Daniel E. Mattox, Jiwon Lee, Wendy Wieland-Alter, Ruth I. Connor, Peter F. Wright, Margaret E. Ackerman, Margaret E. Ackerman, Margaret E. Ackerman   +13 more
doaj   +1 more source

NEUTRALIZATION OF VIRUSES BY HOMOLOGOUS IMMUNE SERUM [PDF]

Journal of Experimental Medicine, 1953
Neutralization experiments with Newcastle disease, influenza A, or bacterial virus, T3, reveal, under all conditions studied, a linear relationship between the logarithm of the serum dilution end point and that of the quantity of virus used. With Newcastle disease and influenza A, the slope of the neutralization line varies markedly with the host-cell ...
David A. J. Tyrrell, Frank L. Horsfall
openaire   +3 more sources

Receptor-Binding Domain (RBD) Antibodies Contribute More to SARS-CoV-2 Neutralization When Target Cells Express High Levels of ACE2

Viruses, 2022
Neutralization assays are experimental surrogates for the effectiveness of infection- or vaccine-elicited polyclonal antibodies and therapeutic monoclonal antibodies targeting SARS-CoV-2.
Ariana Ghez Farrell, Bernadeta Dadonaite, Allison J. Greaney, Rachel Eguia, Andrea N. Loes, Nicholas M. Franko, Jennifer Logue, Juan Manuel Carreño, Anass Abbad, Helen Y. Chu, Kenneth A. Matreyek, Jesse D. Bloom   +11 more
doaj   +1 more source