Predictive Monitoring of Shake Flask Cultures with Online Estimated Growth Models [PDF]
Bioengineering, 2021 Simplicity renders shake flasks ideal for strain selection and substrate optimization in biotechnology. Uncertainty during initial experiments may, however, cause adverse growth conditions and mislead conclusions.
Barbara Pretzner +5 more
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Foam Formation in Shake Flasks and Its Consequences [PDF]
Engineering in Life SciencesFoam formation in stirred tank fermentation processes is a well‐studied phenomenon. However, foaming in shake flask cultivations is rarely considered. Non‐baffled shake flasks, in particular, are generally considered to prevent foaming problems. However,
David Vonester +9 more
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Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations [PDF]
Engineering in Life SciencesFor about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Gesa Brauneck +7 more
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Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Marcel Schrader +15 more
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Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique [PDF]
Journal of Biological Engineering, 2017 Background Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate.
Amizon Azizan, Jochen Büchs
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Application of an Online-Biomass Sensor in an Optical Multisensory Platform Prototype for Growth Monitoring of Biotechnical Relevant Microorganism and Cell Lines in Single-Use Shake Flasks [PDF]
Sensors, 2014 In the context of this work we evaluated a multisensory, noninvasive prototype platform for shake flask cultivations by monitoring three basic parameters (pH, pO2 and biomass).
Christian Ude +5 more
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Analysis and effect of conventional flasks in shaking culture of Escherichia coli [PDF]
AMB Express, 2020 The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO2 and O2 concentrations of bypass type in shake-culture flask.
Masato Takahashi, Hideki Aoyagi
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Modeling bikaverin production by Fusarium oxysporum CCT7620 in shake flask cultures [PDF]
Bioresources and Bioprocessing, 2020 Bikaverin is a fungal red pigment that presents antimicrobial and antitumor activities. Therefore, this substance could be used as an alternative additive in the food and pharmaceutical industries.
Marcela Colombo dos Santos +2 more
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Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2018 Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip +6 more
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Effect of Shaking Speed and Type of Closure on Shake Flask Cultures [PDF]
Applied Microbiology, 1969 Growth of microorganisms and biosynthesis of microbial products in shake flasks may be limited by operating conditions which provide inadequate supplies of oxygen. Methods are described for meeting the oxygen requirements of test organisms by using standard baffled flasks with pad-type closures and shaking at relatively high speeds. Growth
L. E. McDaniel, Edward Bailey
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