Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations [PDF]
Engineering in Life SciencesFor about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Gesa Brauneck +7 more
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Predictive Monitoring of Shake Flask Cultures with Online Estimated Growth Models [PDF]
Bioengineering, 2021 Simplicity renders shake flasks ideal for strain selection and substrate optimization in biotechnology. Uncertainty during initial experiments may, however, cause adverse growth conditions and mislead conclusions.
Barbara Pretzner +5 more
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Foam Formation in Shake Flasks and Its Consequences [PDF]
Engineering in Life SciencesFoam formation in stirred tank fermentation processes is a well‐studied phenomenon. However, foaming in shake flask cultivations is rarely considered. Non‐baffled shake flasks, in particular, are generally considered to prevent foaming problems. However,
David Vonester +9 more
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Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Marcel Schrader +15 more
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Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique [PDF]
Journal of Biological Engineering, 2017 Background Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate.
Amizon Azizan, Jochen Büchs
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Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2018 Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip +6 more
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Systematic evaluation of characteristics of the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2017 Background The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions.
P. Philip +6 more
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Enhanced Biosynthesis of Withanolides by Elicitation and Precursor Feeding in Cell Suspension Culture of Withania somnifera (L.) Dunal in Shake-Flask Culture and Bioreactor [PDF]
PLoS ONE, 2014 The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies.
Ganeshan Sivanandhan, Natesan Selvaraj
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High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure [PDF]
Journal of the Serbian Chemical Society, 2023 Bacillus subtilis is a non-pathogenic, sporulating, Gram-positive bacteria with pronounced antimicrobial and metabolic activity and great potential for wide application in various fields. The aim of this paper was to determine the optimum B.
Stamenković-Stojanović Sandra +4 more
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Brazilian Journal of Pharmaceutical Sciences, 2021 Among the methods described for determining the solubility, shake-flask is suitable to evaluate the equilibrium solubility according to the BCS. Nevertheless, experimental conditions related to the shake-flask method are not well described.
Patrícia Fernanda Monteiro +5 more
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