Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Marcel Schrader +15 more
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Workflow for shake flask and plate cultivations with fats for polyhydroxyalkanoate bioproduction [PDF]
Applied Microbiology and Biotechnology, 2023 Abstract Since natural resources for the bioproduction of commodity chemicals are scarce, waste animal fats (WAF) are an interesting alternative biogenic residual feedstock. They appear as by-product from meat production, but several challenges are related to their application: first, the high melting points (up to 60 ...
Sebastian L. Riedel +6 more
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Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique [PDF]
Journal of Biological Engineering, 2017 Background Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate.
Amizon Azizan, Jochen Büchs
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Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations [PDF]
Engineering in Life SciencesFor about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Gesa Brauneck +7 more
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Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask [PDF]
Microbial Cell Factories, 2018 Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism.
P. Philip +6 more
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Enhanced biosynthesis of withanolides by elicitation and precursor feeding in cell suspension culture of Withania somnifera (L.) Dunal in shake-flask culture and bioreactor. [PDF]
PLoS ONE, 2014 The present study investigated the biosynthesis of major and minor withanolides of Withania somnifera in cell suspension culture using shake-flask culture and bioreactor by exploiting elicitation and precursor feeding strategies.
Ganeshan Sivanandhan +3 more
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Designing Robust Biotechnological Processes Regarding Variabilities using Multi-Objective Optimization Applied to a Biopharmaceutical Seed Train Design [PDF]
, 2022 Development and optimization of biopharmaceutical production processes with cell cultures is cost- and time-consuming and often performed rather empirically. Efficient optimization of multiple-objectives like process time, viable cell density, number of operating steps & cultivation scales, required medium, amount of product as well as product quality ...
arxiv +1 more source
Fermentation, 2022 Phenazine-1-carboxamide (PCN) is effective to control many plant pathogens, and improving PCN production would be of great significance in promoting its development as a biopesticide.
Jiajia Cui +4 more
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Hydrophobicity - Shake Flasks, Protein Folding and Drug Discovery [PDF]
Current Topics in Medicinal Chemistry, 2010 Hydrophobic interactions are some of the most important interactions in nature. They are the primary driving force in a number of phenomena. This is mostly an entropic effect and can account for a number of biophysical events such as protein-protein or protein-ligand binding that are of immense importance in drug design.
Aurijit Sarkar, Glen E. Kellogg
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Shake Flask Biodegradation of 14 Commercial Phthalate Esters [PDF]
Applied and Environmental Microbiology, 1984 An acclimated shake flask CO 2 evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO 2 evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured.
Dean P. O'Grady +4 more
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