Results 241 to 250 of about 22,410 (289)

Reinventing shake flask fermentation: The breathable flask

Biotechnology and Bioengineering
AbstractCultivating cells in shake flasks is a routine operation that is largely unchanged since its inception. A glass or plastic Erlenmeyer vessel with the primary gas exchange taking place across various porous plugs is used with media volumes typically ranging from 100 mL to 2 L.
Govind Rao, Xudong Ge
exaly   +3 more sources

Practices of shake-flask culture and advances in monitoring CO2 and O2

Applied Microbiology and Biotechnology, 2018
About 85 years have passed since the shaking culture was devised. Since then, various monitoring devices have been developed to measure culture parameters. O2 consumed and CO2 produced by the respiration of cells in shaking cultures are of paramount importance due to their presence in both the culture broth and headspace of shake flask.
Masato Takahashi, Hideki Aoyagi, Aoyagi Hideki   +2 more
exaly   +3 more sources

Noninvasive measurement of dissolved oxygen in shake flasks

Biotechnology and Bioengineering, 2002
AbstractShake flasks are ubiquitous in cell culture and fermentation. However, conventional devices for measuring oxygen concentrations are impractical in these systems. Thus, there is no definitive information on the oxygen supply of growing cells. Here we report the noninvasive, nonintrusive monitoring of dissolved oxygen (DO) in shake flasks using a
Leah, Tolosa, Yordan, Kostov, Peter, Harms, Govind, Rao   +3 more
openaire   +2 more sources

Effective shear rates in shake flasks

Chemical Engineering Science, 2014
Abstract In bioprocess development and scale-up and scale-down consistent fermentation conditions in shake flasks and stirred tank reactors are crucial. Fermentation broths at elevated viscosities generally show a non-Newtonian flow behavior. The effective shear rate is known to influence apparent viscosity, and, as a consequence, mixing as well as ...
Heiner Giese, Wolf Klöckner, Carlos Peña, Enrique Galindo, Stefan Lotter, Kai Wetzel, Lena Meissner, Cyril P. Peter, Jochen Büchs   +8 more
openaire   +1 more source

Static shake-flask biotransformation of endothall

Water Research, 1986
Laboratory biotransformation and biodegradation rate coefficients were determined for the aquatic herbicide, endothall. Concentrations of 0.5, 2.0 and 4.0 mg l−1 endothall were employed in a static shake-flask test containing reservoir water. A pseudo-first-order biotransformation rate coefficient of 0.083 day−1 and an aqueous half-life of 8.45 days ...
K.H. Reinert, J.H. Rodgers, T.J. Leslie, M.L. Hinman   +3 more
openaire   +1 more source

Coffee filters as shake-flask closures

Biotechnology Techniques, 1987
Coffee filters are suitable for use as inexpensive and convenient shake-flask closures for propaqating bacteria under aerobic conditions. Gaseous exchange rates are excellent. Sterility is maintained when two layers of filter are used.
Paul A. Hartman, Michael B. Roof
openaire   +1 more source

Model for oxygen transfer in a shake flask

Biotechnology and Bioengineering, 1978
AbstractThe shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the oxygen supply is becoming a growth‐limiting ...
J. C. Van Suijdam, N. W. F. Kossen, A. C. Joha   +2 more
openaire   +1 more source

Parallel substrate feeding and pH-control in shaking-flasks

Biochemical Engineering Journal, 2001
An intermittent feeding system for shaking-flasks was developed to close the gap between batch operated shaking-flasks and fed-batch operated as well as pH-controlled stirred tank reactors. A precise syringe pump was connected via a substrate distribution system to individual 2/2-way miniature valves, one for each of up to 16 shaking-flask. The shaking-
Weuster-Botz, Dirk, Altenbach-Rehm, Jutta, Arnold, Matthias   +2 more
openaire   +3 more sources

In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks

Biotechnology Progress, 2012
AbstractpH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small‐scale culture formats like tissue‐culture flasks and shake flasks. A hydrogel‐based system is reported for in situ pH maintenance without pH measurement in such formats, and is ...
Ketaki, Pradhan, Tejal, Pant, Mugdha, Gadgil   +2 more
openaire   +2 more sources

Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity

Biotechnology and Bioengineering, 2000
In this first article of a series a new method is introduced that enables the accurate determination of the power consumption in a shaking flask. The method is based on torque measurements in the drive and appropriate compensation of the friction losses.
J, Büchs, U, Maier, C, Milbradt, B, Zoels   +3 more
openaire   +2 more sources