Results 251 to 260 of about 22,410 (289)
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Volumetric power consumption in baffled shake flasks
Chemical Engineering Science, 2006Abstract For the cultivation of microorganisms, baffled shake flasks are employed when increased levels of oxygenation and mixing are required. Their use has been discouraged, however, due to the danger of a wetted sterile plug and the lower reproducibility of the experimental results.
Cyril P. Peter +4 more
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Validating shaking flasks as representative screening systems
Biochemical Engineering Journal, 2004The high information demand in biotechnical process development makes shaken bioreactors an essential tool for process development. By far most of all biotechnological experiments are carried out in shaken bioreactors. Nevertheless, the reaction conditions in these systems often are not representative of the following conditions of the technical scale.
Stephan A. Freyer +2 more
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Sphalerite bioleaching comparison in shake flasks and percolators
Minerals Engineering, 2019Abstract Bioleaching experiments with a mixed Acidithiobacillus-culture were carried out in shake flasks as well as in column percolators with crushed and sieved sphalerite-rich ore from a former mine in the German Harz Mountains. Efficient sphalerite dissolution in the bioleaching assays was observed in contrast to the chemical leaching experiments.
Axel Schippers +3 more
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Measurement of the evaporation rate of liquid in a shaking flask
Journal of Fermentation Technology, 1987Abstract The evaporation rate ( N H2O ) of liquid in a shaking flask was measured under various shaking conditions: temperature, humidity, flask shape, liquid volume in the flask ( V L ), length of the stopper in the flask neck ( L C ), rotational speed of the shaker ( N ), and wind velocity ( V W ).
Yasuhiro Sumino, Shun-ichi Akiyama
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Measurement and characterization of mixing time in shake flasks
Chemical Engineering Science, 2011Abstract As yet, investigations on mixing time have been focused on small and large scale stirred tanks. The aim of this study was to develop a new method for characterizing the mixing process and quantitatively measuring the mixing time in shake flasks by introducing a rotating camera for online observation of the fluid flow in combination with a ...
Rung-Kai Tan +2 more
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Evaluation and development of static shake-flask biodegradation test systems
Environmental Toxicology and Chemistry, 1983Abstract Several biodegradation flask systems were evaluated to assess the effects of flask shape and volume and length of incubation on the recovery of 14CO2. During a 6-h incubation period, it was demonstrated that the recovery of 14 CO2 decreased as the volume of the flask (50 ml to 2 L) and the length of the diffusion path increased.
James E. Blum +2 more
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Closure effects on oxygen transfer and aerobic growth in shake flasks
Biotechnology and Bioengineering, 2006AbstractOxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of kGa in shake flasks with closures are introduced. Using Pseudomonas putida, microbial growth on glucose
Hossein, Nikakhtari, Gordon A, Hill
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High-aeration capacity shake-flask system
Journal of Fermentation and Bioengineering, 1989Abstract A novel shake-flask designed for high-aeration capacity, the Tunair flask system, was found to be superior to the currently available shake-flasks in the cultivation of selected microorganisms. The significant features of the flasks include wide-mouth opening, barrel-shaped trunk, two battle types, and dome-shaped closure with vanes.
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Improved shake-flask test for the screening of xanthan-producing microorganisms
World Journal of Microbiology & Biotechnology, 1993Baffled 500 ml Erlenmeyer flasks were compared with conventional 2800 ml Fernbach flasks forXanthomonas campestris to produce xanthan. Bacterial growth rates were similar in both types of flask although the Fernbach flasks gave higher biomass concentrations. Xanthan production was similar in both types of flasks but different viscosities were attained.
E, Galindo +3 more
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Fed‐batch mode in shake flasks by slow‐release technique
Biotechnology and Bioengineering, 2006AbstractMost industrial production processes are performed in fed‐batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode which results in completely different physiological conditions than relevant for production conditions. This may lead to wrong selections of strains.
M, Jeude +6 more
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