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Controlled release of nutrients to mammalian cells cultured in shake flasks
Biotechnology Progress, 2011AbstractThough cell culture‐based protein production processes are rarely carried out under batch mode of operation, cell line and initial process development operations are usually carried out in batch mode due to simplicity of operation in widely used scale down platforms like shake flasks.
Swati Hegde +5 more
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Shake-flask culture of Laccaria laccata , an ectomycorrhizal basidiomycete
Applied Microbiology and Biotechnology, 1996Large-scale exploitation of the potential benefits of ectomycorrhizal fungi in improving plantation yields means that fermentation techniques for these fungi will be required. Starting with a base performance on a rich, complex medium, the effect of variations in some physicochemical culture parameters on biomass yield was studied.
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Shake Flask to Fermentor: What Have We Learned?
Biotechnology Progress, 1998Initially the concern on scale-up of fermentation processes was with oxygen transfer. Strain stability on scale-up was thought to be sufficiently established by serial transfer in shake flasks where conditions were homogeneous. Unfortunately, we now know that there are often mixing problems in large-scale fermentors.
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Characterisation of Shake Flasks for Cultivation of Animal Cell Cultures
2007This study investigated the oxygen transfer processes and general correlations between culture performance and the operating conditions for shake flask fermentations of animal cell cultures. This involved both the online measurement of the oxygen transfer rate and the continuous recording of measurements for the dissolved oxygen concentration in the ...
C. Sauter, J. Haas, G. Jänicke, R. Bux
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Production of High-Titre Diphtheria Toxin in Baffled Shake Flasks
Nature, 1958SUCCESSFUL production of high-titre diphtheria toxin has already been described1, involving aluminium tanks containing 10 or 50 l. of medium. The inoculated medium was stirred at 1,400 r.p.m. so that a vortex was produced, and under these conditions high-titre toxin was obtained in 48 hr.
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Shake-Flask Feedback Controlled Feeding
Genetic Engineering & Biotechnology News, 2013Martin Ischebeck, Manuela Senn
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Fermentation, Oxygen Uptake in Shake-Flask Fermentations
Journal of Agricultural and Food Chemistry, 1953openaire +2 more sources
Innovative Membrane‐Based Fed‐Batch Shake Flask
Chemie Ingenieur Technik, 2016E. P. Philip, Jochen Büchs
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Evolution of Shake Flask Technology
Genetic Engineering & Biotechnology News, 2015openaire +2 more sources