Results 41 to 50 of about 2,552 (164)

Identification of Shiga toxigenic Escherichia coli seropathotypes A and B by multiplex PCR

Molecular and Cellular Probes, 2007
A multiplex PCR assay was developed to identify the six clinically important enterohemorrhagic Escherichia coli (EHEC) serotypes classified in seropathotypes A and B and to differentiate these from Shiga toxigenic E. coli. The assay simultaneously detects genes for Shiga toxin (stx) and intimin (eae), including allelic variants of both genes, 16S ...
S R, Monday, A, Beisaw, P C H, Feng
openaire   +2 more sources

Characterization of Bacteriophages Targeting Non-O157 Shiga Toxigenic Escherichia coli

Journal of Food Protection, 2018
Non-O157 Shiga toxigenic Escherichia coli (STEC) are an important group of foodborne pathogens, implicated in several outbreaks and recalls in the past 2 decades. It is therefore crucial to devise effective control strategies against these pathogens. Bacteriophages present an attractive alternative to conventional pathogen control methods in the food ...
Pushpinder Kaur Litt, Joyjit Saha, Divya Jaroni   +2 more
openaire   +2 more sources

Molecular Surveillance of Shiga Toxigenic Escherichia coli O157 by PulseNet USA

Journal of Food Protection, 2005
PulseNet USA is the national molecular subtyping network system for foodborne disease surveillance. Sixty-four public health and food regulatory laboratories participate in PulseNet USA and routinely perform pulsed-field gel electrophoresis of Shiga toxigenic Escherichia coli isolated from humans, food, water, and the environment on a real-time basis ...
Peter, Gerner-Smidt, Jennifer, Kincaid, Kristy, Kubota, Kelley, Hise, Susan B, Hunter, Mary-Ann, Fair, Dawn, Norton, Ann, Woo-Ming, Terry, Kurzynski, Mark J, Sotir, Marcus, Head, Kristin, Holt, Bala, Swaminathan   +12 more
openaire   +2 more sources

Multiple evolutionary trajectories for non-O157 Shiga toxigenic Escherichia coli [PDF]

, 2019
Abstract Background Shiga toxigenic Escherichia coli (STEC) is an emerging global pathogen and remains a major cause of food-borne illness with more severe symptoms including hemorrhagic colitis and hemolytic-uremic syndrome.
Alikhan, Nabil-Fareed, Bachmann, Nathan L., Zakour, Nouri L. Ben, Petty, Nicola K., Stanton-Cook, Mitchell, Gawthorne, Jayde A., Easton, Donna M., Mahony, Timothy J., Cobbold, Rowland, Schembri, Mark A., Beatson, Scott A.   +10 more
openaire   +1 more source

Association between Shiga Toxin–Producing Escherichia coli O157:H7 stx Gene Subtype and Disease Severity, England, 2009–2019

Emerging Infectious Diseases, 2020
Signs and symptoms of Shiga toxin–producing Escherichia coli (STEC) serogroup O157:H7 infection range from mild gastrointestinal to bloody diarrhea and hemolytic uremic syndrome (HUS).
Lisa Byrne, Natalie Adams, Claire Jenkins   +2 more
doaj   +1 more source

Shiga Toxigenic and Atypical Enteropathogenic Escherichia coli in the Feces and Carcasses of Slaughtered Pigs [PDF]

Foodborne Pathogens and Disease, 2012
Escherichia coli is a pathogen of major importance in swine and public health. To determine the prevalence of Shiga toxigenic E. coli (STEC) and enteropathogenic E. coli (EPEC), samples were collected from the feces and carcasses of swines. In total, 441 samples were collected in four samplings, of which 141 samples tested positive for either the stx1,
Borges, Clarissa Araujo, Beraldo, Livia Gerbasi, Maluta, Renato Pariz, Cardozo, Marita Vedovelli, Cabilio Guth, Beatriz Ernestina, Rigobelo, Everlon Cid, Ávila, Fernando Antonio de   +6 more
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Shiga Toxin–Producing Escherichia coli O157, England and Wales, 1983–2012

Emerging Infectious Diseases, 2016
We evaluated clinical Shiga toxin–producing Escherichia coli O157 infections in England and Wales during 1983–2012 to describe changes in microbiological and surveillance methods.
Natalie L. Adams, Lisa Byrne, Geraldine A. Smith, Richard Elson, John P. Harris, Roland Salmon, Robert Smith, Sarah J. O’Brien, Goutam K. Adak, Claire Jenkins   +9 more
doaj   +1 more source

Virulence Characterization of Shiga-Toxigenic Escherichia coli Isolates from Wholesale Produce [PDF]

Applied and Environmental Microbiology, 2011
ABSTRACT The 13 Shiga-toxigenic Escherichia coli (STEC) strains isolated from wholesale spinach and lettuce consisted mostly of serotypes that have not been implicated in illness. Among these strains, however, were two O113:H21 that carried virulence genes common to this pathogenic serotype ...
Peter C H, Feng, Terry, Councell, Christine, Keys, Steven R, Monday   +3 more
openaire   +2 more sources

Geogenomic Segregation and Temporal Trends of Human Pathogenic Escherichia coli O157:H7, Washington, USA, 2005–2014

Emerging Infectious Diseases, 2018
The often-noted and persistent increased incidence of Escherichia coli O157:H7 infections in rural areas is not well understood. We used a cohort of E.
Gillian A.M. Tarr, Smriti Shringi, Amanda I. Phipps, Thomas E. Besser, Jonathan Mayer, Hanna N. Oltean, Jon Wakefield, Phillip I. Tarr, Peter Rabinowitz   +8 more
doaj   +1 more source

Multiplex PCR to Diagnose Gastrointestinal Infections: Key Limitations in Interpretation

United European Gastroenterology Journal, Volume 14, Issue 7, September 2026.
ABSTRACT Multiplex polymerase chain reaction (PCR) panels are increasingly used for the evaluation of acute diarrhea, providing rapid and sensitive detection of a broad range of enteric pathogens. However, their widespread adoption has created important interpretive challenges for clinicians.
Giannoula S. Tansarli, Ferric C. Fang
wiley   +1 more source