Results 51 to 60 of about 566,210 (290)

Do Read Errors Matter for Genome Assembly?

, 2016
While most current high-throughput DNA sequencing technologies generate short reads with low error rates, emerging sequencing technologies generate long reads with high error rates.
Courtade, Thomas, Shomorony, Ilan, Tse, David   +2 more
core   +1 more source

Ultraaccurate genome sequencing and haplotyping of single human cells. [PDF]

, 2017
Accurate detection of variants and long-range haplotypes in genomes of single human cells remains very challenging. Common approaches require extensive in vitro amplification of genomes of individual cells using DNA polymerases and high-throughput short ...
Bafna, Vineet, Bansal, Vikas, Chu, Wai Keung, Edge, Peter, Huang, Xiaohua, Lee, Ho Suk, Zhang, Kun   +6 more
core   +1 more source

Short-read, high-throughput sequencing technology for STR genotyping

BioTechniques, 2012
DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield nucleotide sequence information.
Daniel M, Bornman, Mark E, Hester, Jared M, Schuetter, Manjula D, Kasoji, Angela, Minard-Smith, Curt A, Barden, Scott C, Nelson, Gene D, Godbold, Christine H, Baker, Boyu, Yang, Jacquelyn E, Walther, Ivan E, Tornes, Pearlly S, Yan, Benjamin, Rodriguez, Ralf, Bundschuh, Michael L, Dickens, Brian A, Young, Seth A, Faith   +17 more
openaire   +2 more sources

Multi-Platform Sequencing Approach Reveals a Novel Transcriptome Profile in Pseudorabies Virus

Frontiers in Microbiology, 2018
Third-generation sequencing is an emerging technology that is capable of solving several problems that earlier approaches were not able to, including the identification of transcripts isoforms and overlapping transcripts. In this study, we used long-read
Norbert Moldován, Dóra Tombácz, Attila Szűcs, Zsolt Csabai, Michael Snyder, Zsolt Boldogkői   +5 more
doaj   +1 more source

Revealing co-infections in pneumonia: A case report on advancing diagnosis with metagenomic sequencing technologies

Journal of Infection and Public Health
Pneumonia, a frequent and serious complication in kidney transplant recipients, is significantly increased by long-term immunosuppressive medication. This case report details a patient with lung cavitation, in which conventional microbiological methods ...
Suzan Alzeer, Reem S. Almaghrabi, Amna Magrashi, Turki Alzahrani, Razan Bakheet, Abdulmohsen Alqasabi, Hamsa Tayeb   +6 more
doaj   +1 more source

Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. [PDF]

, 2018
High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with ...
Byrne, Ashley, Cole, Charles, Green, Richard E, Palmer, Theron, Schmitz, Robert J, Volden, Roger, Vollmers, Christopher   +6 more
core   +1 more source

Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing [PDF]

BMC Genomics, 2009
Abstract Background Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S. cerevisiae.
Yellman Christopher M, Gibson Theodore, Rozowsky Joel, Auerbach Raymond K, Euskirchen Ghia M, Lefrançois Philippe, Gerstein Mark, Snyder Michael   +7 more
openaire   +3 more sources

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

POPULATION SEQUENCING USING SHORT READS: HIV AS A CASE STUDY [PDF]

Biocomputing 2008, 2007
Despite many drawbacks, traditional sequencing technologies have proven to be invaluable in modern medical research, even when the targeted genomes are highly variable. While it is often known in such cases that multiple slightly different sequences are present in the analyzed sample in concentrations that vary dramatically, the traditional techniques ...
Vladimir, Jojic, Tomer, Hertz, Nebojsa, Jojic   +2 more
openaire   +2 more sources

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source