Results 11 to 20 of about 283,388 (253)
Estimation of sequencing error rates in short reads [PDF]
BMC Bioinformatics, 2012 Background Short-read data from next-generation sequencing technologies are now being generated across a range of research projects. The fidelity of this data can be affected by several factors and it is important to have simple and reliable approaches ...
Victoria Xin +4 more
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Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads.
PLoS Computational Biology, 2017 The Illumina DNA sequencing platform generates accurate but short reads, which can be used to produce accurate but fragmented genome assemblies. Pacific Biosciences and Oxford Nanopore Technologies DNA sequencing platforms generate long reads that can ...
Ryan R Wick +3 more
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Calibrating Seed-Based Heuristics to Map Short Reads With Sesame [PDF]
Frontiers in Genetics, 2020 The increasing throughput of DNA sequencing technologies creates a need for faster algorithms. The fate of most reads is to be mapped to a reference sequence, typically a genome.
Guillaume J. Filion +3 more
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Pharmaceutics, 2015 Precision medicine or personalized medicine has been proposed as a modernized and promising medical strategy. Genetic variants of patients are the key information for implementation of precision medicine.
Hao Ye +3 more
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ROCker Models for Reliable Detection and Typing of Short-Read Sequences Carrying β-Lactamase Genes
mSystems, 2022 Identification of genes encoding β-lactamases (BLs) from short-read sequences remains challenging due to the high frequency of shared amino acid functional domains and motifs in proteins encoded by BL genes and related non-BL gene sequences. Divergent BL
Si-Yu Zhang +10 more
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Evaluation of CircRNA Sequence Assembly Methods Using Long Reads
Frontiers in Genetics, 2022 The functional study on circRNAs has been increasing in the past decade due to its important roles in micro RNA sponge, protein coding, the initiation, and progression of diseases.
Jingjing Zhang +8 more
doaj +1 more source
Short paired-end reads trump long single-end reads for expression analysis
BMC Bioinformatics, 2020 Background Typical experimental design advice for expression analyses using RNA-seq generally assumes that single-end reads provide robust gene-level expression estimates in a cost-effective manner, and that the additional benefits obtained from paired ...
Adam H. Freedman +2 more
doaj +1 more source
Chloroplast genome draft assembly of Falcataria moluccana using hybrid sequencing technology
BMC Research Notes, 2023 Objectives Falcataria moluccana, known locally as Sengon, is a fast-growing legume tree that is commonly planted in community forests of Java Island, Indonesia. However, the plantations face attacks of Boktor stem borer (Xystrocera festiva) and gall-rust
Vilda Puji Dini Anita +2 more
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Fragment assembly with short reads [PDF]
Bioinformatics, 2004 Abstract Motivation: Current DNA sequencing technology produces reads of about 500–750 bp, with typical coverage under 10×. New sequencing technologies are emerging that produce shorter reads (length 80–200 bp) but allow one to generate significantly higher coverage (30× and higher) at low cost.
Mark Chaisson +2 more
openaire +3 more sources
A comprehensive investigation of metagenome assembly by linked-read sequencing
Microbiome, 2020 Background The human microbiota are complex systems with important roles in our physiological activities and diseases. Sequencing the microbial genomes in the microbiota can help in our interpretation of their activities.
Lu Zhang +8 more
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