Results 11 to 20 of about 163,355 (309)
Optimized PCR conditions and increased shRNA fold representation improve reproducibility of pooled shRNA screens. [PDF]
PLoS ONE, 2012 RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled shRNA screen, it is imperative to thoroughly optimize experimental conditions to obtain ...
Žaklina Strezoska +10 more
doaj +8 more sources
Molecular Therapy - Nucleic Acids, 2021 In parallel with the expansion of RNA interference (RNAi) techniques, accumulating evidence indicates that RNAi analyses might be seriously biased due to the off-target effects of gene-specific short hairpin RNAs (shRNAs). Our findings indicated that off-
Joanna Bereta +2 more
exaly +3 more sources
Carrier PNA for shRNA delivery into cells [PDF]
Bioorganic & Medicinal Chemistry Letters, 2009 A peptide nucleic acid (PNA)-cell-penetrating peptide (CPP) conjugate (carrier PNA) was used as 'bridge-builder' to connect a CPP with an shRNA. The carrier PNA successfully formed a hybrid with an shRNA bearing complementary dangling bases and the shRNA was introduced into cells by the carrier PNA, and RNAi was induced by the shRNA.
Kitamatsu, Mizuki +5 more
core +8 more sources
Oral Gene Therapy of HFD-Obesity via Nonpathogenic Yeast Microcapsules Mediated shRNA Delivery
Pharmaceutics, 2021 Obesity is a chronic systemic inflammatory disease, which occurs when energy intake exceeds the energy consumption. Therefore, controlling energy intake or increasing physical consumption can effectively control obesity.
Chenxi Xie, Hang Peng, Tongtong Leng
exaly +3 more sources
Antiviral shRNA (for HIV-1) [PDF]
, 2006 Human Immunodeficiency Virus type I (HIV-1) is an RNA virus that causes Acquired Immunodeficiency Syndrome (AIDS). Gene therapy is a new treatment paradigm that aims to modify the patient s cells with therapeutic agents to reduce viral replication and disease progression.
McIntyre, Glen J
openaire +4 more sources
, 2015 This brief protocol shows you how easy it is to get shRNA from demostrated siRNA sequence. With these self-designed shRNA, I've got more than 10 genes knockdown from mammalian cell lines, including mouse embryonic stem cells. Believe it or not, using siRNA sequence from Dharmacon will guarantee you the success.
Vivian Liu
openaire +2 more sources
Anatolian Journal of Cardiology, 2023 Background: Cardiac fibrosis increases with age. Fibroblast activation plays an essential role in cardiac fibrosis. Histone modifications are involved in various chromatin-dependent processes.
Chao Li +8 more
doaj +1 more source
Biology Open, 2022 RNA interference is one of the common methods of studying protein functions. In recent years critical reports have emerged indicating that off-target effects may have a much greater impact on RNAi-based analysis than previously assumed.
Maria Czarnek +3 more
doaj +1 more source
Trakya University Journal of Natural Sciences, 2023 Gammaherpesviruses are associated with multiple types of tumor development and understanding the pathogenesis of these viruses has been the subject of various studies.
Scott Tibbetts, Mehmet Kara
doaj +1 more source
RNAi Degrades the SARS-CoV-2 Spike Protein RNA for Developing Drugs to Treat COVID-19
Nano Biomedicine and Engineering, 2022 COVID-19 is caused by severe acute respiratory SARS-CoV-2. Regardless of the availability of treatment strategies for COVID-19, effective therapy will remain essential.
Weiwei Zhang +17 more
doaj +1 more source