Silencing Human Rb2/p130 with shRNA
Cellular Oncology, 2007 Eleonora Leucci +6 more
doaj +4 more sources
Construction of shRNA lentiviral vector
North American Journal of Medical Sciences, 2010 Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3 ...
Song, Hong, Yang, Ping-Chang
openaire +2 more sources
shRNA-mediated knockdown of syntaxin-4 (Syn4) decreases expression of KCa2.3 at the plasma membrane (PM) in HeLa cells. [PDF]
, 2018 (A) HeLa cells were transfected with either scrambled shRNA (left lane) or shRNA directed against Syn4 (right lane), followed by transfection with KCa2.3 (see Methods). Knockdown of Syn4 was confirmed by immunoblot (bottom blot and panel (D)).
Claudia A. Bertuccio (309866) +5 more
core +1 more source
shRNA and siRNA delivery to the brain [PDF]
Advanced Drug Delivery Reviews, 2007 The limiting factor in in vivo RNA interference (RNAi) is delivery. Drug delivery methods that are effective in cell culture may not be practical in vivo for intravenous RNAi applications. Nucleic acid drugs are highly charged and do not cross cell membranes by free diffusion.
openaire +2 more sources
A Computational Algorithm to Predict shRNA Potency [PDF]
Molecular Cell, 2014 The strength of conclusions drawn from RNAi-based studies is heavily influenced by the quality of tools used to elicit knockdown. Prior studies have developed algorithms to design siRNAs. However, to date, no established method has emerged to identify effective shRNAs, which have lower intracellular abundance than transfected siRNAs and undergo ...
Knott, Simon R.V. +11 more
openaire +2 more sources
Assessment of shRNA-mediated ATAD3 knockdown via lentiviral vectors in mouse adult fibroblasts [PDF]
, 2023 reservedThe aim of this thesis work was to assess and verify the silencing efficiency of short hairpin RNA constructs targeted against ATAD3A mRNA in mouse adult fibroblasts through infection with lentiviral vectors, following cell selection based on ...
GALORA, ANGELA
core
Knocking Down the DRD2 by shRNA Expressing Plasmids in the Nucleus Accumbens Prevented the Disrupting Effect of Apomorphine on Prepulse Inhibition in Rat [PDF]
Journal of Sciences, Islamic Republic of Iran, 2015 Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI.
M.R. Noori-daloii +14 more
doaj
shRNA off-target effects in vivo: impaired endogenous siRNA expression and spermatogenic defects. [PDF]
PLoS ONE, 2015 RNA interference (RNAi) is widely used to determine the function of genes. We chose this approach to assess the collective function of the highly related reproductive homeobox 3 (Rhox3) gene paralogs.
Hye-Won Song +7 more
doaj +1 more source
Safe, long-term hepatic expression of anti-HCV shRNA in a nonhuman primate model [PDF]
, 2012 The hepatitis C virus (HCV) chronically infects 2% of the world population and effective treatment is limited by long duration and significant side-effects. Here, we describe a novel drug, intended as a "single-shot " therapy, which expresses three short
Hayes, K. +41 more
core +1 more source
A) HEK-293FT cells were co-transfected with pENTR/CMV-V5-Myrip and pENTR/U6-shRNA [PDF]
, 2011 Copyright information:Taken from "The Ternary Rab27a–Myrip–Myosin VIIa Complex Regulates Melanosome Motility in the Retinal Pigment Epithelium"Traffic (Copenhagen, Denmark) 2007;8(5):486-499.Published online 26 Mar 2007PMCID:PMC1920545.© 2007 The Authors
Dylan M Owen (57104) +6 more
core +1 more source