Open Life Sciences, 2022 The toll-like receptor 4 (TLR4) pathway is involved in seizures. We investigated whether ultrasound-targeted microbubble destruction (UTMD)-mediated delivery of short hairpin RNA (shRNA) targeting the TLR4 gene (shRNA-TLR4) can reduce ischemia-induced ...
Chen Jia +4 more
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A pipeline for the generation of shRNA transgenic mice [PDF]
Nature Protocols, 2012 RNA interference (RNAi) is an extremely effective tool for studying gene function in almost all metazoan and eukaryotic model systems. RNAi in mice, through the expression of short hairpin RNAs (shRNAs), offers something not easily achieved with traditional genetic approaches-inducible and reversible gene silencing.
Dow, Lukas E +9 more
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Efficiency of H1/U6-shRNA-mediated firefly luciferase (Fluc) knockdown in mice expressing the codon-optimized tetR [PDF]
, 2011 Copyright information:Taken from "Reversible gene knockdown in mice using a tight, inducible shRNA expression system"Nucleic Acids Research 2007;35(7):e54-e54.Published online 21 Mar 2007PMCID:PMC1874634.© 2007 The Author(s) Each configuration (control ...
Frieder Schwenk (27119) +5 more
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Fluorescence-based evaluation of shRNA efficacy [PDF]
Analytical Biochemistry, 2011 RNA interference is a cellular mechanism regulating levels of mRNAs. It has been widely exploited to knock down specific protein targets. The selected interfering RNA sequence greatly influences its ability to knock down the target. Here we present a method for constructing multiple testing plasmids which express small hairpin RNAs (shRNA) targeting ...
B J, Naughton, Dawn D, Han, Howard H, Gu
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Schematic representations of the major steps from pSUPER to concatenable and regulatable multi-shRNA-expression vectors. [PDF]
, 2018 (A) pSUPER. A sequence encoding a specific shRNA would be introduced using the Bgl II and Hin dIII sites. Middle: pSUPER-Sac I (= pSUS). A sequence encoding a specific shRNA would be introduced using the Bgl II and the newly generated Sac I site (in red).
Evelyn Brandt (716779) +7 more
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Expression of plasmid-based shRNA against the E1 and nsP1 genes effectively silenced Chikungunya virus replication. [PDF]
PLoS ONE, 2012 BACKGROUND: Chikungunya virus (CHIKV) is a re-emerging alphavirus that causes chikungunya fever and persistent arthralgia in humans. Currently, there is no effective vaccine or antiviral against CHIKV infection.
Shirley Lam +3 more
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shRNA-mediated knockdown of SNAP23 blocks recycling of KCa2.3 to the plasma membrane (PM) in HeLa cells. [PDF]
, 2018 HeLa cells were transfected with KCa2.3 and PM recycling assessed as detailed in the Methods. (A) In the presence of a scrambled shRNA 61% of KCa2.3 recycled back to the PM after 30 min, as assessed by the loss of KCa2.3 signal.
Claudia A. Bertuccio (309866) +5 more
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Unlocking Therapeutic Potential: Enhanced shRNA Delivery with Tat Peptide in the Human Respiratory Syncytial Virus Treatment [PDF]
Advanced Pharmaceutical BulletinPurpose: This research investigated the development of short hairpin RNA (shRNA) molecules designed to target specific regions of the human respiratory syncytial virus (HRSV) M and F genes.
Saeid Amiri Zadeh Fard +7 more
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shRNA-mediated knockdown of SNAP23 slows Golgi to plasma membrane (PM) delivery of KCa2.3 in HeLa cells. [PDF]
, 2018 (A) HeLa cells were transfected with BLAP-KCa2.3/BirA-KDEL such that KCa2.3 channels are biotinylated in the ER. Neutravidin was used to “block” the KCa2.3 channels at the PM after which newly arrived KCa2.3 channels could be “captured” with streptavidin
Claudia A. Bertuccio (309866) +5 more
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Genetics Research, 2021 Objective. Ultrasound-targeted microbubble destruction (UTMD) technique has recently been developed as a nonviral delivery of gene therapy. This study aimed at investigating the survival and apoptosis of ovarian cancer cell line OVCA-433 by inhibiting ...
Xiaolin Xu +3 more
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