Results 91 to 100 of about 2,579,458 (259)

Genome-wide CRISPR screen reveals key role of sialic acids in PEDV and porcine coronavirus infections

mBio
Porcine epidemic diarrhea virus (PEDV) is a globally distributed alphacoronavirus with economic importance that can cause severe watery diarrhea and even death in piglets.
Guanghao Guo, Mengjia Zhang, Zhuojia Xu, Peng Xi, Hongmei Zhu, Anouk Evers, Robert Jan Lebbink, Yifei Lang, Qigai He, Yao-Wei Huang, Tiehai Li, Berend Jan Bosch, Wentao Li   +12 more
doaj   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source

Glycopolymeric nanoparticles transported by activated platelets protect ischemic myocardium by alleviating PANoptosis through targeting macrophage Dectin‐1

BMEMat, EarlyView.
NP‐SF, designed to target macrophage Dectin‐1 and activated platelets, selectively accumulated in ischemic myocardium, where it was taken up by macrophages after platelet hitchhiking. By inhibiting NLRC5‐related PANoptosis, NP‐SF significantly attenuated pathological damage, and effectively preserved cardiac function in a murine ischemia–reperfusion ...
Guanye Yu, Na Yang, Yan Zhang, Lingxin Peng, Yi Li, Yue Chao, Qinfang Miao, Haikun Qi, Jianhui Zhuang, Weixia Jian, Bingyu Li, Wenhui Peng, Jinghua Chen, Shiyu Gong   +13 more
wiley   +1 more source

Bridging the translational gap in clinical nanomedicine: From rational design to clinical reality

BMEMat, EarlyView.
Nanotechnology offers a multi‐faceted approach to modern healthcare, encompassing high‐sensitivity diagnostic imaging, innovative vaccine delivery, and targeted therapeutics. This review explores how these nanomedicine applications address critical challenges in cancer recurrence and the treatment of neurologic and respiratory diseases to combat rising
Ahmed H. Ghonaim, Marwa Ahmed El‐Naka, Wedad M. Nageeb, Atta Ullah Khan, Noura M. Seleem, Aliaa Abdelghafar, Doaa Zamel, Yang Liu, Qigai He, Wentao Li   +9 more
wiley   +1 more source

Characterization and immunogenicity of nanoparticle vaccines displaying embecovirus spike proteins

Biotechnology Progress, EarlyView.
Virus‐like particle vaccines displaying spike proteins from OC43, HKU1 A, and HKU1 B were evaluated in mice for their ability to elicit antibodies against the spike proteins from OC43, HKU1 A, HKU1 B, and HKU1 C. Abstract Endemic human coronaviruses OC43 and HKU1 cause widespread respiratory infections and can be associated with severe illness in ...
Peter J. Halfmann, Augustine Duffy, Alex Wee, Noah Anderson, Jackson Terrell, Yoshihiro Kawaoka, Ravi S. Kane   +6 more
wiley   +1 more source

Transcriptomic Profiling of SLC and ABC Transporters in the Human Term Placenta

Clinical Pharmacology &Therapeutics, EarlyView.
Solute carriers (SLC) and ATP‐binding cassette (ABC) transporters are essential for placental solute exchange and fetal protection, yet their transcriptomic profiles in the human placenta remain poorly characterized. Although fetal sex influences placental development and function, its impact on transporter expression is unclear.
Elijah Marsh Jung, Nicholas Ieronimakis, Bothaina Mousa, Emily D. Sheikh, Sarah E. Ligon, Joshua W. Monson, Jennifer R. Damicis, Hillary J. Kinsman, Lydia E. Bettridge, Samuel L.M. Arnold, Edward J. Kelly, Jashvant D. Unadkat, Joanne Wang   +12 more
wiley   +1 more source

Velociraptor Machine Learning Quantifies Similarity to Known Cell Types and Matches Cells Across Flow and Imaging Cytometry Platforms

Cytometry Part A, EarlyView.
ABSTRACT Suspension flow cytometry enables high‐throughput cellular profiling at the single cell level, but these data lack positional information. Conversely, tissue‐based imaging cytometry techniques reveal a cell's location within the tissue architecture and can provide insight into cell biology.
Claire E. Cross, Asa A. Brockman, Rebecca A. Ihrie, Jonathan M. Irish   +3 more
wiley   +1 more source

Sialic acid and crystal binding [PDF]

, 2000
Sialic acid and crystal binding.BackgroundWe studied the role of cell surface sialic acid in the adherence of calcium oxalate monohydrate (COM) crystals to Madin-Darby canine kidney (MDCK) cells.MethodsStudies were performed with undifferentiated ...
van der Boom, BG (Burt), Romijn, Hans, Kok, Dik, Kok, Dirk J., van der Boom, Burt G., Verkoelen, Carl F., Verkoelen, CF, Romijn, Johannes C.   +7 more
core   +1 more source

Diagnostic Value of Sialic Acid in Pleural Effusion

Chinese Journal of Lung Cancer, 2010
The present study was conducted in 30 patients of malignant pleural effusion and 30 patients of non malignant pleural effusion. Pleural fluid and blood samples were taken at the time of admission, before starting any treatment.
S KHARB, S TANDON, A BANSAL
doaj  

CardiLect: A combined cross‐species lectin histochemistry protocol for the automated analysis of cardiac remodelling

ESC Heart Failure, Volume 12, Issue 2, Page 1398-1415, April 2025.
Abstract Background Cardiac remodelling, a crucial aspect of heart failure, is commonly investigated in preclinical models by quantifying cardiomyocyte cross‐sectional area (CSA) and microvascular density (MVD) via histological methods, such as immunohistochemistry.
Tamás G. Gergely, Tamás Kovács, Andrea Kovács, Viktória E. Tóth, Nabil V. Sayour, Gábor M. Mórotz, Csenger Kovácsházi, Gábor B. Brenner, Zsófia Onódi, Balázs Enyedi, Domokos Máthé, Przemyslaw Leszek, Zoltán Giricz, Péter Ferdinandy, Zoltán V. Varga   +14 more
wiley   +1 more source