Escherichia coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells via Type 1 Fimbria Bacteria in the Fimbriated State [PDF]
Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E.
Shin, S. +7 more
core +1 more source
Sialidases are gradually entering various areas of human practice—in medicine and pharmacy, as antiviral, antitumor, diagnostic, and vaccine preparations; for the chemoenzymatic synthesis of regioselective sialoglycoconjugates; and for the structural ...
Yana Gocheva +4 more
doaj +1 more source
Visualization of sialoglycoproteins in polyacrylamide gels by acidic ninhydrin reaction. [PDF]
A new method for staining sialoglycoproteins in polyacrylamide gel after disc electrophoresis is described. The method utilizes the reaction of sialic acids with an acidic ninhydrin reagent which yields a stable color with an absorption maximum at 470 nm.
Yao, Kenzabroh +5 more
core
Internalization, intracellular sorting, and recycling of cell surface sialoglycoproteins in HeLa cells [PDF]
Cell surface sialic acid residues were specifically modified to a radiolabeled, neuraminidase-sensitive, 7-carbon analog ([3H]-AcNeu7) by sequential treatment of HeLa or HTC cells with NaBH4, NalO4, and NaBC[3H]4. [3H]-AcNeu7 is neither reutilized nor is
Fishman, Jordan Barry
core
Non polar and affinity monolithic stationary phases for HPLC of large and small molecules and their use in a multi column liquid phase separation platform for the capturing and fractionation of sialoglycoproteins from human serum [PDF]
Scope and Method of Study: The major objective of this investigation was to develop novel nonpolar and affinity monolithic stationary phases for HPLC of small and large molecules, and the evaluation of their potentials in proteomics.
Mayadunne, Erandi Prashani
core
Role of macrophage sialoadhesin in host defense against the sialylated pathogen group B Streptococcus [PDF]
Several bacterial pathogens decorate their surfaces with sialic acid (Sia) residues within cell wall components or capsular exopolysaccharides. Sialic acid expression can promote bacterial virulence by blocking complement activation or by engagement of ...
Joshua Olson +11 more
core +1 more source
The control of sialyltransferase activity in tumor-cell lines derived from different tissues is multifactorial [PDF]
The activities of the sialyltransferase enzymes and the resulting expression of sialoglycoproteins were examined in tumor cells derived from different tissues in order to gain a greater understanding of the factors controlling the cell glycosylation ...
Kieran C. Breen +3 more
core +1 more source
Selective Exo-Enzymatic Labeling Detects Increased Cell Surface Sialoglycoprotein Expression upon Megakaryocytic Differentiation [PDF]
Selective exo-enzymatic labeling (or SEEL) uses recombinant glycosyltransferases and nucleotide-sugar analogues to allow efficient labeling of cell surface glycans.
Medicinal Chemistry and Chemical Biology +10 more
core +1 more source
Characterization of One Phenotype of Human Periodontal Granulation-tissue Fibroblasts [PDF]
Human granulation-tissue fibroblasts were cultured from oral chronic inflammatory lesions and compared with fibroblasts of healthy gingival connective tissue with respect to cell-surface sialoglycoproteins, and the synthesis of extracellular matrix ...
T. Krusius +4 more
core +1 more source
This study aimed to investigate the effects of ammonium pyrrolidinedithiocarbamate (an inhibitor of NF κB activation) and sulforaphane (an inducer of the Nrf2/antioxidant response element (ARE) signaling pathway) on the depolymerization processes of ...
R.M. Ryabushko +2 more
doaj +1 more source

