Results 11 to 20 of about 117,137 (262)

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

Frontiers in Neuroanatomy, 2022
Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated.
Gennadii Piavchenko, Vladislav Soldatov, Artem Venediktov, Natalia Kartashkina, Natalia Novikova, Marina Gorbunova, Tatiana Boronikhina, Alexander Yatskovskiy, Igor Meglinski, Igor Meglinski, Sergey Kuznetsov   +10 more
doaj   +1 more source

Chromosome characterization in Thinopyrum ponticum (Triticeae, Poaceae) using in situ hybridization with different DNA sequences

Genetics and Molecular Biology, 2003
Thinopyrum ponticum (2n = 10x = 70, JJJJsJs) belongs to the Triticeae tribe, and is currently used as a source of pathogen resistance genes in wheat breeding.
Ana Christina Brasileiro-Vidal, Angeles Cuadrado, Sandra P. Brammer, Ana Christina A. Zanatta, Ariano M. Prestes, Maria Irene B. Moraes-Fernandes, Marcelo Guerra   +6 more
doaj   +1 more source

An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

Memorias do Instituto Oswaldo Cruz, 1990
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of
Antonio M. Gonçalves, Nedia S. Nehme, Carlos M. Morel   +2 more
doaj   +1 more source

AN IMPROVED MODIFIED PROTOCOL FOR SILVER STAINING OF DS DNA IN AGAROSE GEL

Pakistan Armed Forces Medical Journal, 2019
Objective: Present study was aimed to develop a reproducible, cheap and sensitive method for silver staining of double stranded DNA in agarose gel. Study Design: Experimental, repeated measure design.
Nuzhat Aisha Ikram, Bushra Iftikhar, Shoaib Ahmed, Fasiha Fatima, Tehmina Qamar, Shakeel R Farooqi   +5 more
doaj   +4 more sources

Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives

Clinical and Experimental Dental Research, 2021
Background It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS). Aim This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application ...
Stefanie Amend, Roland Frankenberger, Christina Boutsiouki, Vanessa Scharrelmann, Julia Winter, Norbert Krämer   +5 more
doaj   +1 more source

A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Genetics and Molecular Biology, 2004
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results ...
Karine Frehner Kavalco, Rubens Pazza
doaj   +1 more source

Investigation of the direct and indirect mechanisms of primary blast insult to the brain

Scientific Reports, 2021
The interaction of explosion-induced blast waves with the head (i.e., a direct mechanism) or with the torso (i.e., an indirect mechanism) presumably causes traumatic brain injury.
Jose E. Rubio, Ginu Unnikrishnan, Venkata Siva Sai Sujith Sajja, Stephen Van Albert, Franco Rossetti, Maciej Skotak, Eren Alay, Aravind Sundaramurthy, Dhananjay Radhakrishnan Subramaniam, Joseph B. Long, Namas Chandra, Jaques Reifman   +11 more
doaj   +1 more source

Detection of point mutations by non-isotopic single strand conformation polymorphism

Brazilian Journal of Medical and Biological Research, 1999
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53.
N.A. Pinheiro, R.P. Moura, E. Monteiro, L.L. Villa   +3 more
doaj   +1 more source

Lipopolysaccharide Purified from Salmonella typhi Sensitizes Human Red Blood Cells at Hemagglutinin concentration, leading to complete Hemolysis upon the addition of Jacalin- a lectin from Jackfruit

Exon
Lipopolysaccharides consist of a hydrophilic-polysaccharide region and a hydrophobic lipid compound known as lipid A. LPS has been reported to cause concentration-dependent agglutination of human RBCs. In this study, the HAP-Hot Aqueous-Phenol Extraction
Logesh Radhakrishnan, V Lavanya, Shazia Jamal, Neesar Ahmed   +3 more
doaj   +1 more source

Periodontal implications of argyrophilic nucleolar organizer region: A mini reviewPeriodontal implications of argyrophilic nucleolar organizer region: A mini review

Journal of Oral Research and Review, 2019
The periodontal lesions with cellular proliferation can be assessed by various methods. One of the methods to determine the proliferative activity is silver-staining argyrophilic nucleolar organizer region (AgNOR) staining.
Vandana Kharidi Laxman, G S Madhushankari, Mini Saluja, Shivani Singh   +3 more
doaj   +1 more source