Results 11 to 20 of about 794,124 (299)
Silver staining the chromosome scaffold
Chromosoma, 1984 Cytological silver-staining procedures reveal the presence of a "core" running along the chromatid axes of isolated HeLa mitotic chromosomes. In this communication we examine the relationship between this "core" and the nonhistone chromosome scaffolding, isolated and characterized in previous publications from this laboratory.
Earnshaw, William C. +1 more
openaire +4 more sources
Silver Staining of Proteins in 2DE Gels [PDF]
, 2009 Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and ...
Lelong, Cécile +3 more
openaire +7 more sources
Silver staining of proteins in polyacrylamide gels [PDF]
Nature Protocols, 2006 Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of
Chevallet, Mireille +2 more
openaire +7 more sources
A silver impregnation method for motor and sensory nerves and their endings in formalin-fixed mammalian muscles [PDF]
, 1988 A silver impregnation method is described which shows motor and sensory nerves and their endings in formalin-fixed mammalian muscles. The method works with the same reliability on flattened muscle pieces as well as on frozen sections. Large nerve bundles,
Kaupenjohann, Heike, Schweizer, Hermann
core +1 more source
Frontiers in Neuroanatomy, 2022 Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated.
Gennadii Piavchenko +10 more
doaj +1 more source
Analysis by high-resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells [PDF]
, 1990 HL-60 leukemic cells were differentiated along the neutrophilic pathway with retinoic acid (RA) or along the monocytic pathway with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3).
Seifert, Roland, Jungblut, P. R.
core +1 more source
Genetics and Molecular Biology, 2003 Thinopyrum ponticum (2n = 10x = 70, JJJJsJs) belongs to the Triticeae tribe, and is currently used as a source of pathogen resistance genes in wheat breeding.
Ana Christina Brasileiro-Vidal +6 more
doaj +1 more source
An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
Memorias do Instituto Oswaldo Cruz, 1990 A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of
Antonio M. Gonçalves +2 more
doaj +1 more source
AN IMPROVED MODIFIED PROTOCOL FOR SILVER STAINING OF DS DNA IN AGAROSE GEL
Pakistan Armed Forces Medical Journal, 2019 Objective: Present study was aimed to develop a reproducible, cheap and sensitive method for silver staining of double stranded DNA in agarose gel. Study Design: Experimental, repeated measure design.
Nuzhat Aisha Ikram +5 more
doaj +4 more sources
A rapid alternative technique for obtaining silver-positive patterns in chromosomes
Genetics and Molecular Biology, 2004 Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results ...
Karine Frehner Kavalco, Rubens Pazza
doaj +1 more source