Results 11 to 20 of about 116,983 (264)

Silver staining the chromosome scaffold

open access: yesChromosoma, 1984
Cytological silver-staining procedures reveal the presence of a "core" running along the chromatid axes of isolated HeLa mitotic chromosomes. In this communication we examine the relationship between this "core" and the nonhistone chromosome scaffolding, isolated and characterized in previous publications from this laboratory.
Earnshaw, William C.   +1 more
openaire   +3 more sources

Chromosome characterization in Thinopyrum ponticum (Triticeae, Poaceae) using in situ hybridization with different DNA sequences

open access: yesGenetics and Molecular Biology, 2003
Thinopyrum ponticum (2n = 10x = 70, JJJJsJs) belongs to the Triticeae tribe, and is currently used as a source of pathogen resistance genes in wheat breeding.
Ana Christina Brasileiro-Vidal   +6 more
doaj   +1 more source

An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

open access: yesMemorias do Instituto Oswaldo Cruz, 1990
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of
Antonio M. Gonçalves   +2 more
doaj   +1 more source

Microleakage of pit and fissure sealings placed after enamel conditioning with phosphoric acid or with self‐etching primers/adhesives

open access: yesClinical and Experimental Dental Research, 2021
Background It is still not fully understood what pretreatment is best for achieving maximum tightness for pit and fissure sealings (PFS). Aim This study investigated microleakage of PFS placed after etching with phosphoric acid or after the application ...
Stefanie Amend   +5 more
doaj   +1 more source

A rapid alternative technique for obtaining silver-positive patterns in chromosomes

open access: yesGenetics and Molecular Biology, 2004
Silver nitrate chromosome staining to evidence nucleolar organizer regions (NORs) is a widely adopted methodology. The aim of the present work was to improve this technique, reducing the preparation time without decreasing the quality of the results ...
Karine Frehner Kavalco, Rubens Pazza
doaj   +1 more source

Investigation of the direct and indirect mechanisms of primary blast insult to the brain

open access: yesScientific Reports, 2021
The interaction of explosion-induced blast waves with the head (i.e., a direct mechanism) or with the torso (i.e., an indirect mechanism) presumably causes traumatic brain injury.
Jose E. Rubio   +11 more
doaj   +1 more source

Detection of point mutations by non-isotopic single strand conformation polymorphism

open access: yesBrazilian Journal of Medical and Biological Research, 1999
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53.
N.A. Pinheiro   +3 more
doaj   +1 more source

Periodontal implications of argyrophilic nucleolar organizer region: A mini reviewPeriodontal implications of argyrophilic nucleolar organizer region: A mini review

open access: yesJournal of Oral Research and Review, 2019
The periodontal lesions with cellular proliferation can be assessed by various methods. One of the methods to determine the proliferative activity is silver-staining argyrophilic nucleolar organizer region (AgNOR) staining.
Vandana Kharidi Laxman   +3 more
doaj   +1 more source

Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae) [PDF]

open access: yesComparative Cytogenetics, 2021
In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time on Ophrys tenthredinifera Willdenow, 1805, Serapias vomeracea (Burman f., 1770) Briquet, 1910 and Himantoglossum hircinum (Linnaeus, 1753) Sprengel,
Alessio Turco   +4 more
doaj   +3 more sources

Lipopolysaccharide Purified from Salmonella typhi Sensitizes Human Red Blood Cells at Hemagglutinin concentration, leading to complete Hemolysis upon the addition of Jacalin- a lectin from Jackfruit

open access: yesExon
Lipopolysaccharides consist of a hydrophilic-polysaccharide region and a hydrophobic lipid compound known as lipid A. LPS has been reported to cause concentration-dependent agglutination of human RBCs. In this study, the HAP-Hot Aqueous-Phenol Extraction
Logesh Radhakrishnan   +3 more
doaj   +1 more source

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