Results 21 to 30 of about 117,137 (262)

Visualizing the Osteocyte Lacuno-Canalicular System via a Rapid 10-Minute Silver Nitrate Staining Method

open access: yesBio-Protocol
The conventional Ploton silver method employs a high-concentration 50% (w/v; 2.943 mol/L) silver nitrate solution for histological staining and characterization of the osteocyte lacuno-canalicular system (LCS).
Jinlian Wu, Weiwei Dai, Libo Wang
doaj   +1 more source

Investigation on the cytotoxicity and apoptosis of citrate based silver nanoparticles, in vitro [PDF]

open access: yesJournal of Health Sciences and Surveillance System, 2018
Background: With the advancement of nanotechnology, nanoparticles have been applied in our modern society. However, the hazardous effects of nanoparticles on organisms have not been thoroughly clarified yet.
Laya Ebrahimi   +3 more
doaj   +1 more source

Amelioration Strategies for Silver Diamine Fluoride: Moving from Black to White

open access: yesAntibiotics, 2023
Topical cariostatic agents have become a reasonable alternative for managing dental caries in young children. Silver diamine fluoride (SDF) is a practical topical approach to arrest caries and avoid extensive and risky dental treatment.
Amjad Almuqrin   +4 more
doaj   +1 more source

Silver Staining of Proteins in 2DE Gels [PDF]

open access: yes, 2009
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and ...
Lelong, Cécile   +3 more
openaire   +5 more sources

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

open access: yesMolecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh   +12 more
wiley   +1 more source

A novel optimized silver nitrate staining method for visualizing the osteocyte lacuno-canalicular system

open access: yesFrontiers in Endocrinology
A 50% (w/v, equivalent to 2.943 mol/L) silver nitrate solution is commonly used to stain and characterize the osteocyte lacuno-canalicular system (LCS) in bone biology research.
Jinlian Wu   +8 more
doaj   +1 more source

Modification of AgNOR staining to reveal the nucleolus in thick sections specified for stereological and pathological assessments of brain tissue

open access: yesHeliyon, 2019
Background: Various stains have been devised to reveal degenerative or reactive cell phenotypes, or the disintegrative and/or neuropathic lesions associated with Alzheimer's, Parkinson's, and Pick's diseases, Down's syndrome, or chemical toxicity ...
S.O. Ahmad   +4 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

open access: yesMolecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis   +3 more
wiley   +1 more source

Adaptation of the agyrophil technique for nucleolar organizer regions to canine peripheral blood smears [PDF]

open access: yesOpen Veterinary Journal, 2018
Nucleolar organizer regions (NORs) are chromosomal segments which are selectively stained by silver methods and evaluated as agyrophilic nucleolar organizer regions (AgNORs).
John Ogunsola, Richard Antia
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

open access: yesMolecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu   +13 more
wiley   +1 more source

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