Results 51 to 60 of about 1,956 (160)
Production and Purification of Simian Vacuolating Virus40 virus-like particles
Simian virus 40 (SV40) is a member of the Polyomaviridae family .Express the structural protein VP1 of SV40 in Bac-to-Bac baculovirus expression system. The expressed protein will be self-assembled to SV40 virus-like particles (SV40 VLPs).
LIU Rui-Xi +6 more
doaj
Abstract Aquaculture is one of the fastest‐growing food production sectors. As the global human population continues to increase and further pressure is added to the prospects of achieving global food security, aquaculture is expected to play an integral role in meeting future nutrition demands.
Megan N. Moran +4 more
wiley +1 more source
Equine whole blood and plasma samples from an administration of AAV‐GFP viral vector were stored at −20°C for 8 months and analysed with qPCR and massively parallel sequencing. The vector detection in stored plasma samples followed the same detection profile as the fresh plasma samples with minimal degradation. Stored blood samples showed lower overall
Jillian Maniego +7 more
wiley +1 more source
Polyomavirus T antigens activate an antiviral state [PDF]
The large T antigen (TAg) of the polyomavirus Simian virus 40 (SV40) is known to play an important role in several events during the viral life cycle, including DNA replication, transcription, and virion assembly.
Giacobbi, Nicholas
core
Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster [PDF]
We recently described a drug-based selectable and counterselectable genetic platform for the animal model system Drosophila melanogaster, consisting of four resistance and two sensitivity markers that allow direct selection for, or counterselection ...
Dierick, Herman A +4 more
core +2 more sources
Abstract Aims The clinical application of doxorubicin (DOX), a potent anthracycline anticancer drug that effectively treats various malignancies, is limited by its side effects, such as cardiomyopathy. Apurinic/apyrimidinic endonuclease 1/redox factor‐1 (APE1/Ref‐1) is a multifunctional protein that can be secreted and is a promising target for the ...
Soo Yeon An +6 more
wiley +1 more source
Research and Practice in Thrombosis and Haemostasis, Volume 5, Issue S2, October 2021.
wiley +1 more source
Optogenetic confirmation of transverse‐tubular membrane excitability in intact cardiac myocytes
Abstract figure legend Methodological framework employed to dissect the electrical properties of isolated t‐tubules (TT). Isolated cardiomyocytes with specific expression of ChR2 (ChR2 CM) are initially stained with a voltage‐sensitive dye (VSD) suitable for two‐photon imaging, thus allowing TT labelling and imaging.
Marina Scardigli +16 more
wiley +1 more source
The cell nucleus is separated from the cytoplasm by a double membrane, and transport of molecules occurs through aqueous channels called nuclear pore complexes. Here, we combined quantitative binding assays and X‐ray crystallography to study the mechanistic details of how phosphorylation can influence the interaction of a protein with the nuclear ...
Emily M. Cross +7 more
wiley +1 more source
The Modulation of Apoptotic Pathways by Gammaherpesviruses [PDF]
Apoptosis or programmed cell death is a tightly regulated process fundamental for cellular development and elimination of damaged or infected cells during the maintenance of cellular homeostasis. It is also an important cellular defense mechanism against
Prerna Dabral +4 more
core +3 more sources

