Results 1 to 10 of about 75,693 (242)

Efficient immortalization of primary nasopharyngeal epithelial cells for EBV infection study. [PDF]

PLoS ONE, 2013
Nasopharyngeal carcinoma (NPC) is common among southern Chinese including the ethnic Cantonese population living in Hong Kong. Epstein-Barr virus (EBV) infection is detected in all undifferentiated type of NPC in this endemic region.
Yim Ling Yip, Pei Shin Pang, Wen Deng, Chi Man Tsang, Musheng Zeng, Pok Man Hau, Cornelia Man, Yuesheng Jin, Anthony Po Wing Yuen, Sai Wah Tsao   +9 more
doaj   +7 more sources

Oxidative Metabolism Drives Immortalization of Neural Stem Cells during Tumorigenesis

Cell, 2020
Metabolic reprogramming is a key feature of many cancers, but how and when it contributes to tumorigenesis remains unclear. Here we demonstrate that metabolic reprogramming induced by mitochondrial fusion can be rate-limiting for immortalization of tumor-
Ana Veloso, Merve Deniz Abdusselamoglu, Sunanjay Bajaj   +2 more
exaly   +2 more sources

Quercetin Interrupts the Positive Feedback Loop Between STAT3 and IL-6, Promotes Autophagy, and Reduces ROS, Preventing EBV-Driven B Cell Immortalization

Biomolecules, 2019
The oncogenic gammaherpesvirus Epstein−Barr virus (EBV) immortalizes in vitro B lymphocytes into lymphoblastoid cell lines (LCLs), a model that gives the opportunity to explore the molecular mechanisms driving viral tumorigenesis. In this study, we
Marisa Granato, Maria Saveria Gilardini Montani, Claudia Zompetta, Roberta Santarelli, Roberta Gonnella, Maria Anele Romeo, Gabriella D’Orazi, Alberto Faggioni, Mara Cirone   +8 more
doaj   +2 more sources

Cancer-associated TERT promoter mutations abrogate telomerase silencing [PDF]

eLife, 2015
Mutations in the human telomerase reverse transcriptase (TERT) promoter are the most frequent non-coding mutations in cancer, but their molecular mechanism in tumorigenesis has not been established.
Kunitoshi Chiba, Joshua Z Johnson, Jacob M Vogan, Tina Wagner, John M Boyle, Dirk Hockemeyer   +5 more
doaj   +3 more sources

Telomerase Immortalization of Human Corneal Endothelial Cells Yields Functional Hexagonal Monolayers [PDF]

PLoS ONE, 2012
Human corneal endothelial cells (HCEnCs) form a monolayer of hexagonal cells whose main function is to maintain corneal clarity by regulating corneal hydration. HCEnCs are derived from neural crest and are arrested in the post-mitotic state.
Bonanno, Joseph, Chen, Yuming, Jurkunas, Ula V., Li, Shimin, Nguyen, Tracy T., Schmedt, Thore   +5 more
core   +15 more sources

A Rapid Method to Immortalize Schwann Cells [PDF]

Pediatric Discovery
The study of cells aids in comprehending the pathophysiology of diseases. However, obtaining a large number of primary cells in a short period is challenging, and they senesce and die after repeated passages.
YanTing Zhang, Jian Zheng, Yingling Yao, Ling He, Shaoyan Liang, Guoxin Nan   +5 more
doaj   +2 more sources

Establishment and Characterization of an Immortalized Oviduct Epithelial Cell Line from Yak (Bos grunniens) [PDF]

Animals
Oviduct epithelial cells (OECs) constitute a critical component of the oviductal mucosa, providing essential microenvironmental support for fertilization and early embryonic development.
Wenyi Cai, Yuting Jiang, Xuelian Xu, Qiliang Ma, Congcong Xu, Wei Fu, Daoliang Lan   +6 more
doaj   +2 more sources

Establishment of an immortalized Muscovy duck embryo fibroblast cell line and exploration of virus tropism [PDF]

Poultry Science
Muscovy duck embryo fibroblasts (MDEF) are a useful cellular model for studying the molecular mechanisms associated with waterfowl viruses infection. However, the short lifespan and poor passaging capacity of MDEF limit their application.
Li Zeng, Dandan Jiang, Guangju You, Xiaoxia Cheng, Shaoying Chen, Shao Wang, Shilong Chen   +6 more
doaj   +2 more sources

The Generation of a Testicular Peritubular Cell Line from Giant Pandas (Ailuropoda melanoleuca) [PDF]

Cells
Giant pandas (Ailuropoda melanoleuca), a flagship endangered species under priority preservation in China, remain poorly understood in terms of their testicular physiology and the mechanisms underlying spermatogenesis. Testicular peritubular cells (TPTCs)
Xueni You, Yuren Wang, Yuliang Liu, Rong Hou, Yi Zheng, Junhui An   +5 more
doaj   +2 more sources

Longitudinal assessment of DNA methylation changes during HPVE6E7-induced immortalization of primary keratinocytes

Epigenetics, 2015
Jan M Kooter, Saskia M Wilting, Wim Quint   +2 more
exaly   +2 more sources