Results 121 to 130 of about 1,214,396 (338)

Complex Analysis of Single-Cell RNA Sequencing Data

Biochemistry (Moscow), 2023
Single-cell RNA sequencing (scRNA-seq) is a revolutionary tool for studying the physiology of normal and pathologically altered tissues. This approach provides information about molecular features (gene expression, mutations, chromatin accessibility, etc.) of cells, opens up the possibility to analyze the trajectories/phylogeny of cell differentiation ...
Khozyainova, Anna A., Valyaeva, Anna A., Arbatsky, Mikhail S., Isaev, Sergey V., Iamshchikov, Pavel S., Volchkov, Egor V., Sabirov, Marat S., Zainullina, Viktoria R., Chechekhin, Vadim I., Vorobev, Rostislav S., Menyailo, Maxim E., Tyurin-Kuzmin, Pyotr A., Denisov, Evgeny V.   +12 more
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer

Nature Communications
Single-cell RNA sequencing predominantly employs short-read sequencing to characterize cell types, states and dynamics; however, it is inadequate for comprehensive characterization of RNA isoforms. Long-read sequencing technologies enable single-cell RNA
Ashley Byrne, Daniel Le, Kostianna Sereti, Hari Menon, Samir Vaidya, Neha Patel, Jessica Lund, Ana Xavier-Magalhães, Minyi Shi, Yuxin Liang, Timothy Sterne-Weiler, Zora Modrusan, William Stephenson   +12 more
doaj   +1 more source

Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA. [PDF]

, 2018
High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with ...
Byrne, Ashley, Cole, Charles, Green, Richard E, Palmer, Theron, Schmitz, Robert J, Volden, Roger, Vollmers, Christopher   +6 more
core   +1 more source

Advancements in single-cell RNA sequencing and spatial transcriptomics: transforming biomedical research

Acta Biochimica Polonica
In recent years, significant advancements in biochemistry, materials science, engineering, and computer-aided testing have driven the development of high-throughput tools for profiling genetic information.
Getnet Molla Desta, A. Birhanu
semanticscholar   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing

Nature Communications, 2018
Cancer-associated fibroblasts (CAFs) are a major constituent of the tumor microenvironment, although their origin and roles in shaping disease initiation, progression and treatment response remain unclear due to significant heterogeneity. Here, following
M. Bartoschek, N. Oskolkov, M. Bocci, J. Lövrot, C. Larsson, M. Sommarin, C. Madsen, David Lindgren, G. Pekar, G. Karlsson, M. Ringnér, J. Bergh, Åsa K. Björklund, K. Pietras   +13 more
semanticscholar   +1 more source

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

Nature Communications, 2020
Recent developments in stem cell biology have enabled the study of cell fate decisions in early human development that are impossible to study in vivo. However, understanding how development varies across individuals and, in particular, the influence of ...
Anna S. E. Cuomo, D. Seaton, Davis J McCarthy, I. Martinez, M. Bonder, J. Garcia-Bernardo, Shradha Amatya, Pedro Madrigal, Abigail Isaacson, F. Buettner, A. Knights, K. Natarajan, L. Vallier, J. Marioni, Mariya Chhatriwala, O. Stegle   +15 more
semanticscholar   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source