Results 71 to 80 of about 980,999 (298)

Virtual Environment for Next Generation Sequencing Analysis [PDF]

, 2012
Next Generation Sequencing technology, on the one hand, allows a more accurate analysis, and, on the other hand, increases the amount of data to process.
Abate, Francesco, Acquaviva, Andrea, Mossucca, L., Provenzano, R., Terzo, Olivier   +4 more
core  

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Single cell RNA sequencing: modern approaches and achievements

RUDN Journal of Medicine
Relevance. Single-cell RNA sequencing (scRNA-seq) is a modern approach to studying the diversity and heterogeneity of RNA transcripts in individual cells, as well as to identifying the composition of different cell types and functions in organisms ...
Artem E. Gusev, Petr V. Chernov, Nikolai A. Dmitriev, Ilya A. Kofiadi   +3 more
doaj   +1 more source

Single-cell transcriptome sequencing: recent advances and remaining challenges [version 1; referees: 2 approved]

F1000Research, 2016
Single-cell RNA-sequencing methods are now robust and economically practical and are becoming a powerful tool for high-throughput, high-resolution transcriptomic analysis of cell states and dynamics.
Serena Liu, Cole Trapnell
doaj   +1 more source

The lncRNA landscape of breast cancer reveals a role for DSCAM-AS1 in breast cancer progression. [PDF]

, 2016
Molecular classification of cancers into subtypes has resulted in an advance in our understanding of tumour biology and treatment response across multiple tumour types. However, to date, cancer profiling has largely focused on protein-coding genes, which
Backofen, Rolf, Cao, Xuhong, Chinnaiyan, Arul M, Collins, Colin, Feng, Felix Y, Gawronski, Alexander, Han, Sumin, Hayes, Daniel F, Hosono, Yasuyuki, Iyer, Matthew K, Kregel, Steven, Ma, Teng, Malik, Rohit, Niknafs, Yashar S, Pierce, Lori J, Pitchiaya, Sethuramasundaram, Poliakov, Anton, Prensner, John R, Rae, James M, Sahinalp, Cenk S, Siebenaler, Ronald F, Singhal, Udit, Speers, Corey, Uhl, Michael, Wilder-Romans, Kari, Xiao, Lanbo, Zhang, Chao, Zhao, Shuang G   +27 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer

Nature Communications
Single-cell RNA sequencing predominantly employs short-read sequencing to characterize cell types, states and dynamics; however, it is inadequate for comprehensive characterization of RNA isoforms. Long-read sequencing technologies enable single-cell RNA
Ashley Byrne, Daniel Le, Kostianna Sereti, Hari Menon, Samir Vaidya, Neha Patel, Jessica Lund, Ana Xavier-Magalhães, Minyi Shi, Yuxin Liang, Timothy Sterne-Weiler, Zora Modrusan, William Stephenson   +12 more
doaj   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source