Results 21 to 30 of about 5,030,666 (315)

The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts

Nature Communications, 2016
In response to intracellular signals, bacterial translational riboswitches embedded in mRNAs can regulate gene expression through inhibition of translation initiation.
Arlie J. Rinaldi, Paul E. Lund, Mario R. Blanco, Nils G. Walter   +3 more
doaj   +1 more source

Verification of Single-Molecule Imaging and Single-Molecule Measurements

Journal of Robotics and Mechatronics, 2010
Single-molecule imaging and single-molecule measurements constitute an integral part of life science researches. The research method, which allows direct measurements of individual motions and events of biomolecules, has contributed much to the development of life science research by providing us with numerous findings. It is left up to the judgment of
Akihiko Ishijima, Hajime Fukuoka, Yuichi Inoue   +2 more
openaire   +1 more source

Future of biomedical sciences: Single molecule microscopy [PDF]

, 2007
The behavior of single molecule defines whether a cell lives, dies, or responds to a specific drug treatment. Single molecule microscopies have begun to reveal the number, location, and functionalities of molecules outside and inside living cells.
Walter, Nils G.
core   +1 more source

Single-molecule-Biophysics-UOW/rapid_singleTether: Rapid single-molecule characterisation of enzymes involved in nucleic-acid metabolism. Zenodo Upload

, 2022
All supplementary code for "Rapid single-molecule characterisation of DNA ...
StefanHMueller
core   +1 more source

Single molecule detection for in vitro diagnostics

, 2022
Paper 68620HIn this paper we present a novel highly sensitive detection system for diagnostic applications. The system is designed to meet the needs of medical diagnostics for reliable measurements of pathogens and biomarkers in the low concentration ...
Greiner, B., Mathis, H.P., Giehring, S., Ackermann, J., Tonn, T., Kirner, T., Kukoc-Zivojnov, N., Tschachojan, D.   +7 more
core   +1 more source

Resolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution

Cell Reports, 2017
Summary: Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions.
Sethuramasundaram Pitchiaya, Laurie A. Heinicke, Jun I. Park, Elizabeth L. Cameron, Nils G. Walter   +4 more
doaj   +1 more source

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

An intermolecular FRET sensor detects the dynamics of T cell receptor clustering

Nature Communications, 2017
Cellular signalling is often facilitated by membrane protein clustering, but detection of protein clustering at high spatiotemporal resolution is challenging.
Yuanqing Ma, Elvis Pandzic, Philip R. Nicovich, Yui Yamamoto, Joanna Kwiatek, Sophie V. Pageon, Aleš Benda, Jérémie Rossy, Katharina Gaus   +8 more
doaj   +1 more source

CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

iScience, 2021
Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann   +8 more
doaj   +1 more source

Pulling on single molecules [PDF]

Nature Methods, 2012
Single-molecule methods to apply and measure force and displacement are expanding mechanobiology.
openaire   +2 more sources