Results 21 to 30 of about 6,486,155 (264)

Resolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution

Cell Reports, 2017
Summary: Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions.
Sethuramasundaram Pitchiaya, Laurie A. Heinicke, Jun I. Park, Elizabeth L. Cameron, Nils G. Walter   +4 more
doaj   +1 more source

Visualizing helicases unwinding DNA at the single molecule level. [PDF]

, 2010
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes ...
Fili, Natali, Mashanov, Gregory I, Batters, C, Dillingham, MS, Yeeles, Joseph T P, Toseland, Christopher P, Mashanov, GI, Toseland, CP, Wallace, Mark I, Webb, Martin R, Molloy, JE, Batters, Christopher, Webb, MR, Wallace, Mark, Fili, N, Dillingham, Mark S, Yeeles, JT, Molloy, Justin E   +17 more
core   +1 more source

An intermolecular FRET sensor detects the dynamics of T cell receptor clustering

Nature Communications, 2017
Cellular signalling is often facilitated by membrane protein clustering, but detection of protein clustering at high spatiotemporal resolution is challenging.
Yuanqing Ma, Elvis Pandzic, Philip R. Nicovich, Yui Yamamoto, Joanna Kwiatek, Sophie V. Pageon, Aleš Benda, Jérémie Rossy, Katharina Gaus   +8 more
doaj   +1 more source

CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

iScience, 2021
Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting.
Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann   +8 more
doaj   +1 more source

Single-molecule detection on a portable 3D-printed microscope

Nature Communications, 2019
Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting ...
James W. P. Brown, Arnaud Bauer, Mark E Polinkovsky, Akshay Bhumkar, Dominic J. B. Hunter, Katharina Gaus, Emma Sierecki, Yann Gambin   +7 more
doaj   +1 more source

Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

STAR Protocols, 2023
Summary: Multiplexed high-density label super-resolution microscopy image reconstruction by integrating exchangeable single-molecule localization (IRIS) enables elucidating fine structures and molecular distribution in cells and tissues.
Qianli Zhang, Akitoshi Miyamoto, Naoki Watanabe   +2 more
doaj   +1 more source

Decoding the Structural Dynamics and Conformational Alternations of DNA Secondary Structures by Single-Molecule FRET Microspectroscopy

Frontiers in Molecular Biosciences, 2021
In addition to the canonical double helix form, DNA is known to be extrapolated into several other secondary structural patterns involving themselves in inter- and intramolecular type hydrogen bonding. The secondary structures of nucleic acids go through
Debolina Bandyopadhyay, Debolina Bandyopadhyay, Padmaja P. Mishra, Padmaja P. Mishra   +3 more
doaj   +1 more source

A Memristive Element Based on an Electrically Controlled Single‐Molecule Reaction [PDF]

, 2020
A single-molecule memory element is electrically-controlled using two distinct reaction mechanisms as reported by K. Moth-Poulsen, J. Hihath, and co-workers (DOI:10.1002/anie.202002300).
Haipeng B. Li, Joshua Hihath, Behabitu E. Tebikachew, Li, Haipeng B., Tebikachew, Behabitu Ergette, Kasper Moth‐Poulsen, Tebikachew, Behabitu Ergette,, Hihath, Joshua,, Hihath, Joshua, Wiberg, Cedrik, Cedrik Wiberg, Moth-Poulsen, Kasper,, Li, Haipeng B.,, Wiberg, Cedrik,, Moth-Poulsen, Kasper   +14 more
core   +1 more source

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays

Nature Communications, 2020
Fluorogenic RNA aptamers have been used for RNA imaging, but folding and fluorescence stability often limited their use in high resolution applications.
Adam D. Cawte, Peter J. Unrau, David S. Rueda   +2 more
doaj   +1 more source

Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis

Cell Structure and Function, 2023
In eukaryotic motile cells, the active Ras (Ras-GTP)-enriched domain is generated in an asymmetric manner on the cell membrane through the excitable dynamics of an intracellular signaling network.
Da Young Shin, Hiroaki Takagi, Michio Hiroshima, Satomi Matsuoka, Masahiro Ueda   +4 more
doaj   +1 more source